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Regulation of Somatic Cell Fate by Escargot in Drosophila Testis
Regulation of Somatic Cell Fate by Escargot in Drosophila Testis
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152000
- ISBN
- 9798382829777
- DDC
- 574
- 서명/저자
- Regulation of Somatic Cell Fate by Escargot in Drosophila Testis
- 발행사항
- [Sl] : University of California, Los Angeles, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 59 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
- 주기사항
- Advisor: Long, Jeffrey Aaron;Jones, D. Leanne.
- 학위논문주기
- Thesis (Ph.D.)--University of California, Los Angeles, 2024.
- 초록/해제
- 요약Adult stem cells are crucial for tissue homeostasis and repair. Their fate is tightly regulated by intrinsic genetic factors and extrinsic cues within the stem cell niche. These niches are adaptable, responding to stimuli to maintain homeostasis, but the molecular details of this regulation are not fully understood. This study explores a mechanism that regulates the fate of somatic support cells in the Drosophila testis to maintain tissue homeostasis.The hub in the Drosophila testis niche secretes factors that regulate germline stem cells (GSCs) and cyst stem cells (CySCs). Our lab previously reported that the Snail family transcriptional regulator Escargot (Esg) is necessary for maintaining the identity of both CySCs and hub cells. Depleting esg from hub cells leads to their conversion into CySCs, resulting in the loss of both GSC and CySC populations. Similarly, esg depletion from early somatic cells causes CySCs to differentiate prematurely into daughter cyst cells. These findings indicate that Esg is central to determining somatic cell fates, although the mechanisms are not fully understood.In the adult testis, Egfr activity is low in hub cells under homeostatic conditions but active in CySCs and early cyst cells, regulating their differentiation. Interestingly, Esg protein levels are significantly higher in hub cells than in cyst cells. We hypothesized that Esg influences somatic cell fate by repressing the Egfr signaling cascade. Our results show that repressing Egfr signaling suppresses hub-to-CySC conversion upon esg depletion. Conversely, overexpressing esg in CySCs increases the number of early cyst cells and induces CySC-to-hub cell conversion, which depends on Egfr activity. Constitutive Egfr activation in CySCs prevents this conversion upon esg overexpression. These findings support the hypothesis that Escargot acts upstream of Egfr to regulate somatic cell identity and maintain tissue homeostasis in the testis.
- 일반주제명
- Cellular biology
- 일반주제명
- Genetics
- 일반주제명
- Molecular biology
- 키워드
- Drosophila
- 키워드
- Escargot
- 키워드
- Somatic cells
- 키워드
- Stem cell
- 키워드
- Genetic factors
- 기타저자
- University of California, Los Angeles Molecular Biology 0573
- 기본자료저록
- Dissertations Abstracts International. 85-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
008250123s2024 us c eng d■001000017162329
■00520250211152000
■006m o d
■007cr#unu||||||||
■020 ▼a9798382829777
■035 ▼a(MiAaPQ)AAI31329701
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aKryza, Jordan Ryan.
■24510▼aRegulation of Somatic Cell Fate by Escargot in Drosophila Testis
■260 ▼a[Sl]▼bUniversity of California, Los Angeles▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a59 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-12, Section: B.
■500 ▼aAdvisor: Long, Jeffrey Aaron;Jones, D. Leanne.
■5021 ▼aThesis (Ph.D.)--University of California, Los Angeles, 2024.
■520 ▼aAdult stem cells are crucial for tissue homeostasis and repair. Their fate is tightly regulated by intrinsic genetic factors and extrinsic cues within the stem cell niche. These niches are adaptable, responding to stimuli to maintain homeostasis, but the molecular details of this regulation are not fully understood. This study explores a mechanism that regulates the fate of somatic support cells in the Drosophila testis to maintain tissue homeostasis.The hub in the Drosophila testis niche secretes factors that regulate germline stem cells (GSCs) and cyst stem cells (CySCs). Our lab previously reported that the Snail family transcriptional regulator Escargot (Esg) is necessary for maintaining the identity of both CySCs and hub cells. Depleting esg from hub cells leads to their conversion into CySCs, resulting in the loss of both GSC and CySC populations. Similarly, esg depletion from early somatic cells causes CySCs to differentiate prematurely into daughter cyst cells. These findings indicate that Esg is central to determining somatic cell fates, although the mechanisms are not fully understood.In the adult testis, Egfr activity is low in hub cells under homeostatic conditions but active in CySCs and early cyst cells, regulating their differentiation. Interestingly, Esg protein levels are significantly higher in hub cells than in cyst cells. We hypothesized that Esg influences somatic cell fate by repressing the Egfr signaling cascade. Our results show that repressing Egfr signaling suppresses hub-to-CySC conversion upon esg depletion. Conversely, overexpressing esg in CySCs increases the number of early cyst cells and induces CySC-to-hub cell conversion, which depends on Egfr activity. Constitutive Egfr activation in CySCs prevents this conversion upon esg overexpression. These findings support the hypothesis that Escargot acts upstream of Egfr to regulate somatic cell identity and maintain tissue homeostasis in the testis.
■590 ▼aSchool code: 0031.
■650 4▼aCellular biology
■650 4▼aGenetics
■650 4▼aMolecular biology
■653 ▼aDrosophila
■653 ▼aEscargot
■653 ▼aSomatic cells
■653 ▼aStem cell
■653 ▼aGenetic factors
■690 ▼a0379
■690 ▼a0369
■690 ▼a0307
■71020▼aUniversity of California, Los Angeles▼bMolecular Biology 0573.
■7730 ▼tDissertations Abstracts International▼g85-12B.
■790 ▼a0031
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17162329▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
