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Glutamine Synthetase: Metals and Filament Coordination
Glutamine Synthetase: Metals and Filament Coordination
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152803
- ISBN
- 9798384094760
- DDC
- 574
- 서명/저자
- Glutamine Synthetase: Metals and Filament Coordination
- 발행사항
- [Sl] : University of Washington, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 73 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-03, Section: B.
- 주기사항
- Advisor: Kollman, Jusitn.
- 학위논문주기
- Thesis (Ph.D.)--University of Washington, 2024.
- 초록/해제
- 요약Glutamine has a central role in cellular metabolism because it is a key donor of carbon and nitrogen atoms to other key metabolic precursors. Nitrogen fixation and mobilization is controlled through the inter-conversion of glutamate and glutamine by glutamine synthetase (GS). GS converts glutamate and ammonium into glutamine in an ATP-dependent reaction. GS assembles into filaments whose formation is conserved among E. coli, yeast, and plants. The higher-order structural organization observed among GS's and the importance of the glutamine pool within metabolism suggest these filaments may serve as a layer of regulation as seen with other filament-forming enzymes. Here we characterized in vitro assembly of E. coli and human glutamine synthetase. We show that the filament formation of E. coli GS is pH and divalent cation-dependent. Additionally, we show metals disrupt active site integrity through secondary structure changes. Finally, we formally show human GS can form filaments, define the residues involved in assembly, and provide structural evidence that these filaments bind and turnover substrates.
- 일반주제명
- Biochemistry
- 일반주제명
- Cellular biology
- 일반주제명
- Molecular biology
- 키워드
- Glutamine
- 키워드
- Ammonium
- 키워드
- Metals
- 기타저자
- University of Washington Molecular and Cellular Biology
- 기본자료저록
- Dissertations Abstracts International. 86-03B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211152803
■006m o d
■007cr#unu||||||||
■020 ▼a9798384094760
■035 ▼a(MiAaPQ)AAI31556853
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aMuniz, Richard Sammy.
■24510▼aGlutamine Synthetase: Metals and Filament Coordination
■260 ▼a[Sl]▼bUniversity of Washington▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a73 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-03, Section: B.
■500 ▼aAdvisor: Kollman, Jusitn.
■5021 ▼aThesis (Ph.D.)--University of Washington, 2024.
■520 ▼aGlutamine has a central role in cellular metabolism because it is a key donor of carbon and nitrogen atoms to other key metabolic precursors. Nitrogen fixation and mobilization is controlled through the inter-conversion of glutamate and glutamine by glutamine synthetase (GS). GS converts glutamate and ammonium into glutamine in an ATP-dependent reaction. GS assembles into filaments whose formation is conserved among E. coli, yeast, and plants. The higher-order structural organization observed among GS's and the importance of the glutamine pool within metabolism suggest these filaments may serve as a layer of regulation as seen with other filament-forming enzymes. Here we characterized in vitro assembly of E. coli and human glutamine synthetase. We show that the filament formation of E. coli GS is pH and divalent cation-dependent. Additionally, we show metals disrupt active site integrity through secondary structure changes. Finally, we formally show human GS can form filaments, define the residues involved in assembly, and provide structural evidence that these filaments bind and turnover substrates.
■590 ▼aSchool code: 0250.
■650 4▼aBiochemistry
■650 4▼aCellular biology
■650 4▼aMolecular biology
■653 ▼aGlutamine
■653 ▼aAmmonium
■653 ▼aGlutamine synthetase
■653 ▼aMetals
■653 ▼aFilament formation
■690 ▼a0487
■690 ▼a0379
■690 ▼a0307
■71020▼aUniversity of Washington▼bMolecular and Cellular Biology.
■7730 ▼tDissertations Abstracts International▼g86-03B.
■790 ▼a0250
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17163867▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
