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The Chlamydia trachomatis Inc Tri1 Interacts With the Host Protein TRAF7 to Modulate TRAF7-Dependent Interactions
The Chlamydia trachomatis Inc Tri1 Interacts With the Host Protein TRAF7 to Modulate TRAF7-Dependent Interactions
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151001
- ISBN
- 9798381971675
- DDC
- 574
- 서명/저자
- The Chlamydia trachomatis Inc Tri1 Interacts With the Host Protein TRAF7 to Modulate TRAF7-Dependent Interactions
- 발행사항
- [Sl] : University of California, San Francisco, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 89 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-09, Section: B.
- 주기사항
- Advisor: Mukherjee, Shaeri.
- 학위논문주기
- Thesis (Ph.D.)--University of California, San Francisco, 2024.
- 초록/해제
- 요약Like any successful intracellular pathogen, Chlamydia trachomatis must overcome several host defenses to complete its developmental cycle. Chlamydia is thought to accomplish this through the employment of effector proteins, particularly a unique class of effectors which are inserted in the Chlamydial inclusion membrane. To test the hypothesis that these inclusion membrane proteins (Incs) target host cell proteins, our lab conducted an affinity purification-mass spectrometry screen (AP-MS) in which cells were transfected with individually tagged Incs. The predicted interaction between the Inc Tri1 and the host ubiquitin ligase TRAF7 is the subject of this work.In this dissertation, we characterize the Tri:TRAF7 complex during infection and reveal that Tri1 can disrupt native TRAF7 protein-protein interactions (PPIs, Chapter 2). Through additional analysis of this TRAF7 interactome dataset, we revealed that Tri1 can also promote novel TRAF7 PPIs (Chapter 3). Finally, we conducted RNAseq analysis to determine the role of Tri1 during infection and determined that it may play a modest role in altering signaling pathways. Overall, this work contributes to the growing literature on C. trachomatis Incs and highlights the important roles they may play during infection.
- 일반주제명
- Biology
- 일반주제명
- Microbiology
- 일반주제명
- Cellular biology
- 일반주제명
- Molecular biology
- 일반주제명
- Biochemistry
- 키워드
- Infection
- 기타저자
- University of California, San Francisco Biochemistry and Molecular Biology
- 기본자료저록
- Dissertations Abstracts International. 85-09B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798381971675
■035 ▼a(MiAaPQ)AAI30994050
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aHerrera, Clara M.▼0(orcid)0000-0002-1791-084X
■24510▼aThe Chlamydia trachomatis Inc Tri1 Interacts With the Host Protein TRAF7 to Modulate TRAF7-Dependent Interactions
■260 ▼a[Sl]▼bUniversity of California, San Francisco▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a89 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-09, Section: B.
■500 ▼aAdvisor: Mukherjee, Shaeri.
■5021 ▼aThesis (Ph.D.)--University of California, San Francisco, 2024.
■520 ▼aLike any successful intracellular pathogen, Chlamydia trachomatis must overcome several host defenses to complete its developmental cycle. Chlamydia is thought to accomplish this through the employment of effector proteins, particularly a unique class of effectors which are inserted in the Chlamydial inclusion membrane. To test the hypothesis that these inclusion membrane proteins (Incs) target host cell proteins, our lab conducted an affinity purification-mass spectrometry screen (AP-MS) in which cells were transfected with individually tagged Incs. The predicted interaction between the Inc Tri1 and the host ubiquitin ligase TRAF7 is the subject of this work.In this dissertation, we characterize the Tri:TRAF7 complex during infection and reveal that Tri1 can disrupt native TRAF7 protein-protein interactions (PPIs, Chapter 2). Through additional analysis of this TRAF7 interactome dataset, we revealed that Tri1 can also promote novel TRAF7 PPIs (Chapter 3). Finally, we conducted RNAseq analysis to determine the role of Tri1 during infection and determined that it may play a modest role in altering signaling pathways. Overall, this work contributes to the growing literature on C. trachomatis Incs and highlights the important roles they may play during infection.
■590 ▼aSchool code: 0034.
■650 4▼aBiology
■650 4▼aMicrobiology
■650 4▼aCellular biology
■650 4▼aMolecular biology
■650 4▼aBiochemistry
■653 ▼aChlamydia trachomatis
■653 ▼aInclusion membrane proteins
■653 ▼aInfection
■653 ▼aProtein-protein interactions
■653 ▼aSignaling pathways
■690 ▼a0306
■690 ▼a0379
■690 ▼a0487
■690 ▼a0410
■690 ▼a0307
■71020▼aUniversity of California, San Francisco▼bBiochemistry and Molecular Biology.
■7730 ▼tDissertations Abstracts International▼g85-09B.
■790 ▼a0034
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160347▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
