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Understanding the Role of DNA Discontinuities in Promoting Meiotic Crossing Over
Understanding the Role of DNA Discontinuities in Promoting Meiotic Crossing Over
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152644
- ISBN
- 9798384051053
- DDC
- 574
- 저자명
- Payero, Lisette.
- 서명/저자
- Understanding the Role of DNA Discontinuities in Promoting Meiotic Crossing Over
- 발행사항
- [Sl] : Cornell University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 179 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-03, Section: B.
- 주기사항
- Advisor: Alani, Eric.
- 학위논문주기
- Thesis (Ph.D.)--Cornell University, 2024.
- 초록/해제
- 요약Meiosis is a specialized form of cell division that results in the formation of gametes. In most sexually reproducing organisms, meiotic recombination is a crucial step in meiosis during which homologous chromosomes physically interact and exchange genetic information. The final DNA recombination intermediate in this process is the double Holliday Junction (dHJ). A major question in the meiosis field is the mechanism through which dHJs are cleaved in a biased manner to create crossover products. Mlh1-Mlh3, the nuclease responsible for resolving the majority of dHJs in budding yeast, is not intrinsically capable of recognizing and cleaving dHJs in a biased manner. This observation, combined with genetic screens revealing a wide variety of crossover promoting factors, indicates that other proteins may interact at the dHJ to promote biased resolution. Here, I describe work demonstrating genetic evidence in baker's yeast that the Rad2/XPG family nuclease Exo1 promotes meiotic crossing over by protecting DNA nicks from ligation. I describe findings that structural elements in Exo1 that interact with DNA, such as those required for the bending of DNA during nick/flap recognition, are critical for its role in crossing over. In addition, meiotic overexpression of Cdc9 ligase reduces the crossover levels of exo1 DNA-binding mutants to levels that approach the exo1 null. I synthesize these observations to propose a model in which temporally protected, dHJ-associated nicks are key features of biased resolution. Finally, I describe a novel technique for mapping meiotic DNA nicks genome-wide in wild-type, exo1Δ, and other mutant yeast strains. Using these data, I will interrogate existing models for crossover resolution and provide valuable insight into the structure of meiotic dHJs.
- 일반주제명
- Biochemistry
- 일반주제명
- Cellular biology
- 일반주제명
- Molecular biology
- 일반주제명
- Genetics
- 키워드
- Meiosis
- 키워드
- Chromosomes
- 기타저자
- Cornell University Biochemistry Molecular and Cell Biology
- 기본자료저록
- Dissertations Abstracts International. 86-03B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211152644
■006m o d
■007cr#unu||||||||
■020 ▼a9798384051053
■035 ▼a(MiAaPQ)AAI31485585
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aPayero, Lisette.▼0(orcid)0000-0002-5919-5928
■24510▼aUnderstanding the Role of DNA Discontinuities in Promoting Meiotic Crossing Over
■260 ▼a[Sl]▼bCornell University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a179 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-03, Section: B.
■500 ▼aAdvisor: Alani, Eric.
■5021 ▼aThesis (Ph.D.)--Cornell University, 2024.
■520 ▼aMeiosis is a specialized form of cell division that results in the formation of gametes. In most sexually reproducing organisms, meiotic recombination is a crucial step in meiosis during which homologous chromosomes physically interact and exchange genetic information. The final DNA recombination intermediate in this process is the double Holliday Junction (dHJ). A major question in the meiosis field is the mechanism through which dHJs are cleaved in a biased manner to create crossover products. Mlh1-Mlh3, the nuclease responsible for resolving the majority of dHJs in budding yeast, is not intrinsically capable of recognizing and cleaving dHJs in a biased manner. This observation, combined with genetic screens revealing a wide variety of crossover promoting factors, indicates that other proteins may interact at the dHJ to promote biased resolution. Here, I describe work demonstrating genetic evidence in baker's yeast that the Rad2/XPG family nuclease Exo1 promotes meiotic crossing over by protecting DNA nicks from ligation. I describe findings that structural elements in Exo1 that interact with DNA, such as those required for the bending of DNA during nick/flap recognition, are critical for its role in crossing over. In addition, meiotic overexpression of Cdc9 ligase reduces the crossover levels of exo1 DNA-binding mutants to levels that approach the exo1 null. I synthesize these observations to propose a model in which temporally protected, dHJ-associated nicks are key features of biased resolution. Finally, I describe a novel technique for mapping meiotic DNA nicks genome-wide in wild-type, exo1Δ, and other mutant yeast strains. Using these data, I will interrogate existing models for crossover resolution and provide valuable insight into the structure of meiotic dHJs.
■590 ▼aSchool code: 0058.
■650 4▼aBiochemistry
■650 4▼aCellular biology
■650 4▼aMolecular biology
■650 4▼aGenetics
■653 ▼aMeiosis
■653 ▼aDouble Holliday Junction
■653 ▼aMeiotic crossing over
■653 ▼aChromosomes
■653 ▼aMeiotic recombination
■690 ▼a0487
■690 ▼a0379
■690 ▼a0369
■690 ▼a0307
■71020▼aCornell University▼bBiochemistry, Molecular and Cell Biology.
■7730 ▼tDissertations Abstracts International▼g86-03B.
■790 ▼a0058
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17163251▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
