Optimizing Retron-Based Genome Engineering Across the Kingdoms of Life
Optimizing Retron-Based Genome Engineering Across the Kingdoms of Life
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211153035
- ISBN
- 9798346876755
- DDC
- 575
- 저자명
- Crawford, Kate.
- 서명/저자
- Optimizing Retron-Based Genome Engineering Across the Kingdoms of Life
- 발행사항
- [Sl] : University of California, San Francisco, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 176 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-06, Section: B.
- 주기사항
- Includes supplementary digital materials.
- 주기사항
- Advisor: Shipman, Seth.
- 학위논문주기
- Thesis (Ph.D.)--University of California, San Francisco, 2024.
- 초록/해제
- 요약Since the discovery that CRISPR-Cas9 is an RNA-guided DNA-endonuclease and can perform programmable cutting, the genome engineering field has moved from making a simple double-stranded break towards performing a precise edit, where you change the identity of one or many nucleotides to another. However, making a precise repair requires a template for that repair, often made out of single-stranded DNA. In this dissertation, I will detail optimization of one such tool for intracellular DNA production, the bacterial retron, and its utilization as a template for precise repair in: eukaryotes (Chapter 2), bacteriophage (Chapter 3), and then high-throughput libraries for higher rates of precise editing in human cells (Chapter 4).
- 일반주제명
- Genetics
- 일반주제명
- Bioengineering
- 키워드
- Bacteriophage
- 키워드
- Editrons
- 키워드
- Retrons
- 기타저자
- University of California, San Francisco Bioengineering
- 기본자료저록
- Dissertations Abstracts International. 86-06B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798346876755
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■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a575
■1001 ▼aCrawford, Kate.▼0(orcid)0009-0002-8915-0648
■24510▼aOptimizing Retron-Based Genome Engineering Across the Kingdoms of Life
■260 ▼a[Sl]▼bUniversity of California, San Francisco▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a176 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-06, Section: B.
■500 ▼aIncludes supplementary digital materials.
■500 ▼aAdvisor: Shipman, Seth.
■5021 ▼aThesis (Ph.D.)--University of California, San Francisco, 2024.
■520 ▼aSince the discovery that CRISPR-Cas9 is an RNA-guided DNA-endonuclease and can perform programmable cutting, the genome engineering field has moved from making a simple double-stranded break towards performing a precise edit, where you change the identity of one or many nucleotides to another. However, making a precise repair requires a template for that repair, often made out of single-stranded DNA. In this dissertation, I will detail optimization of one such tool for intracellular DNA production, the bacterial retron, and its utilization as a template for precise repair in: eukaryotes (Chapter 2), bacteriophage (Chapter 3), and then high-throughput libraries for higher rates of precise editing in human cells (Chapter 4).
■590 ▼aSchool code: 0034.
■650 4▼aGenetics
■650 4▼aBioengineering
■653 ▼aBacteriophage
■653 ▼aEditrons
■653 ▼aGenome engineering
■653 ▼aHigh-throughput libraries
■653 ▼aRetrons
■690 ▼a0369
■690 ▼a0202
■71020▼aUniversity of California, San Francisco▼bBioengineering.
■7730 ▼tDissertations Abstracts International▼g86-06B.
■790 ▼a0034
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17164717▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
