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Exploration and Development of Xylopyrinde Fluorescent Tools for Spatiotemporal Imaging
Exploration and Development of Xylopyrinde Fluorescent Tools for Spatiotemporal Imaging
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152030
- ISBN
- 9798384024569
- DDC
- 540
- 서명/저자
- Exploration and Development of Xylopyrinde Fluorescent Tools for Spatiotemporal Imaging
- 발행사항
- [Sl] : University of Pennsylvania, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 239 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-02, Section: B.
- 주기사항
- Advisor: Chenoweth, David M.
- 학위논문주기
- Thesis (Ph.D.)--University of Pennsylvania, 2024.
- 초록/해제
- 요약Xylopyridine (affectionately referred to as Xylo), represents a class of small molecule fluorescent dyes known for their ability to irreversibly convert their spectral properties upon irradiation with UV light. This quality, known as photoconvertibility, belongs to a series of mechanisms we describe as photorespsonsive (PR) fluorescence. Photoactivatability and photoswitchability represent two other possible mechanisms of photoresponsive fluorescence. Photoresponsive fluorescent proteins (PRFPs) have enabled a variety of dynamic imaging applications including spatiotemporal tracking of cells, organelles, and proteins of interest. However, they are limited by their large size, poor photostability, and propensity to oligomerize. Photoresponsive small molecules (PRSMs) hold the promise of greatly improving the future of fluorescence microscopy by addressing these issues and have already enabled markedly improved performance for spatiotemporal and sub diffraction limit imaging of cells, cellular structures, and biomolecules. This thesis will describe the development and exploration of the Xylopyridine scaffold as a PRSM platform capable of improving the spatiotemporal imaging of crowded biological systems. An improved synthetic strategy for accessing the Xylo core will be described followed by rapid diversification facilitated by its ease of access. Novel photoconvertible molecules based on the Xylopyridine scaffold will then be evaluated for their spatiotemporal imaging performance. Finally, spatiotemporal imaging of cholesterol within living cells will be discussed to showcase the paradigm shifting capability of Xylopyridine fluorescent tools.
- 일반주제명
- Chemistry
- 일반주제명
- Cellular biology
- 일반주제명
- Biochemistry
- 일반주제명
- Biophysics
- 키워드
- Xylopyridine
- 키워드
- Dyes
- 기타저자
- University of Pennsylvania Chemistry
- 기본자료저록
- Dissertations Abstracts International. 86-02B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798384024569
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■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a540
■1001 ▼aJohnny, Christopher L.
■24510▼aExploration and Development of Xylopyrinde Fluorescent Tools for Spatiotemporal Imaging
■260 ▼a[Sl]▼bUniversity of Pennsylvania▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a239 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-02, Section: B.
■500 ▼aAdvisor: Chenoweth, David M.
■5021 ▼aThesis (Ph.D.)--University of Pennsylvania, 2024.
■520 ▼aXylopyridine (affectionately referred to as Xylo), represents a class of small molecule fluorescent dyes known for their ability to irreversibly convert their spectral properties upon irradiation with UV light. This quality, known as photoconvertibility, belongs to a series of mechanisms we describe as photorespsonsive (PR) fluorescence. Photoactivatability and photoswitchability represent two other possible mechanisms of photoresponsive fluorescence. Photoresponsive fluorescent proteins (PRFPs) have enabled a variety of dynamic imaging applications including spatiotemporal tracking of cells, organelles, and proteins of interest. However, they are limited by their large size, poor photostability, and propensity to oligomerize. Photoresponsive small molecules (PRSMs) hold the promise of greatly improving the future of fluorescence microscopy by addressing these issues and have already enabled markedly improved performance for spatiotemporal and sub diffraction limit imaging of cells, cellular structures, and biomolecules. This thesis will describe the development and exploration of the Xylopyridine scaffold as a PRSM platform capable of improving the spatiotemporal imaging of crowded biological systems. An improved synthetic strategy for accessing the Xylo core will be described followed by rapid diversification facilitated by its ease of access. Novel photoconvertible molecules based on the Xylopyridine scaffold will then be evaluated for their spatiotemporal imaging performance. Finally, spatiotemporal imaging of cholesterol within living cells will be discussed to showcase the paradigm shifting capability of Xylopyridine fluorescent tools.
■590 ▼aSchool code: 0175.
■650 4▼aChemistry
■650 4▼aCellular biology
■650 4▼aBiochemistry
■650 4▼aBiophysics
■653 ▼aSpatiotemporal imaging
■653 ▼aXylopyridine
■653 ▼aPhotorespsonsive fluorescence
■653 ▼aPhotoresponsive fluorescent proteins
■653 ▼aDyes
■690 ▼a0485
■690 ▼a0786
■690 ▼a0379
■690 ▼a0487
■71020▼aUniversity of Pennsylvania▼bChemistry.
■7730 ▼tDissertations Abstracts International▼g86-02B.
■790 ▼a0175
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17162589▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
