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An Actomyosin Network Organizes Niche Morphology and Responds to Feedback From Recruited Stem Cells
An Actomyosin Network Organizes Niche Morphology and Responds to Feedback From Recruited Stem Cells
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152701
- ISBN
- 9798384023265
- DDC
- 574
- 서명/저자
- An Actomyosin Network Organizes Niche Morphology and Responds to Feedback From Recruited Stem Cells
- 발행사항
- [Sl] : University of Pennsylvania, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 85 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-02, Section: B.
- 주기사항
- Advisor: DiNardo, Stephen.
- 학위논문주기
- Thesis (Ph.D.)--University of Pennsylvania, 2024.
- 초록/해제
- 요약Stem cells often rely on signals from a niche, which in many tissues adopts a precise morphology. What remains elusive is how niches are formed, and how niche morphology impacts its function. To address this, we leverage the Drosophila gonadal niche, which affords genetic tractability and live-imaging. We have previously shown mechanisms dictating niche cell migration to their appropriate position within the gonad, and the resultant consequences on niche function. Here, utilizing a combination of fixed and live-imaging approaches, we show that once positioned, niche cells robustly polarize filamentous actin (F-actin) and Non-muscle Myosin II (MyoII) towards neighboring germ cells. We utilize laser severing of the actomyosin network to reveal polarized actomyosin tension along the niche periphery. By manipulating actomyosin contractility (AMC) with a combination of pharmacological and transgenic approaches, we find that this tension generates a highly reproducible smoothened niche contour. Without contractility, niches are misshapen and exhibit functional defects in their ability to limit self-renewal signals and regulate germline stem cell divisions. We additionally examined the upstream mechanisms that polarize MyoII along the niche periphery. By ablating germ cells and inhibiting their division, we show that germ cells aid in polarizing MyoII within niche cells, and that extrinsic input is required for niche morphogenesis and function. Our work reveals a feedback mechanism where stem cells shape the niche that guides their behavior.
- 일반주제명
- Cellular biology
- 일반주제명
- Genetics
- 일반주제명
- Developmental biology
- 일반주제명
- Molecular biology
- 키워드
- Drosophila
- 키워드
- Morphogenesis
- 키워드
- Niche
- 키워드
- Stem cell
- 기타저자
- University of Pennsylvania Cell and Molecular Biology
- 기본자료저록
- Dissertations Abstracts International. 86-02B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■007cr#unu||||||||
■020 ▼a9798384023265
■035 ▼a(MiAaPQ)AAI31488015
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aWarder, Bailey Nicole.
■24513▼aAn Actomyosin Network Organizes Niche Morphology and Responds to Feedback From Recruited Stem Cells
■260 ▼a[Sl]▼bUniversity of Pennsylvania▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a85 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-02, Section: B.
■500 ▼aAdvisor: DiNardo, Stephen.
■5021 ▼aThesis (Ph.D.)--University of Pennsylvania, 2024.
■520 ▼aStem cells often rely on signals from a niche, which in many tissues adopts a precise morphology. What remains elusive is how niches are formed, and how niche morphology impacts its function. To address this, we leverage the Drosophila gonadal niche, which affords genetic tractability and live-imaging. We have previously shown mechanisms dictating niche cell migration to their appropriate position within the gonad, and the resultant consequences on niche function. Here, utilizing a combination of fixed and live-imaging approaches, we show that once positioned, niche cells robustly polarize filamentous actin (F-actin) and Non-muscle Myosin II (MyoII) towards neighboring germ cells. We utilize laser severing of the actomyosin network to reveal polarized actomyosin tension along the niche periphery. By manipulating actomyosin contractility (AMC) with a combination of pharmacological and transgenic approaches, we find that this tension generates a highly reproducible smoothened niche contour. Without contractility, niches are misshapen and exhibit functional defects in their ability to limit self-renewal signals and regulate germline stem cell divisions. We additionally examined the upstream mechanisms that polarize MyoII along the niche periphery. By ablating germ cells and inhibiting their division, we show that germ cells aid in polarizing MyoII within niche cells, and that extrinsic input is required for niche morphogenesis and function. Our work reveals a feedback mechanism where stem cells shape the niche that guides their behavior.
■590 ▼aSchool code: 0175.
■650 4▼aCellular biology
■650 4▼aGenetics
■650 4▼aDevelopmental biology
■650 4▼aMolecular biology
■653 ▼aActomyosin contractility
■653 ▼aDrosophila
■653 ▼aMorphogenesis
■653 ▼aNiche
■653 ▼aStem cell
■690 ▼a0379
■690 ▼a0369
■690 ▼a0758
■690 ▼a0307
■71020▼aUniversity of Pennsylvania▼bCell and Molecular Biology.
■7730 ▼tDissertations Abstracts International▼g86-02B.
■790 ▼a0175
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17163386▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
