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Mapping Lineage Dynamics of Progenitors in the Human Cerebral Cortex
Mapping Lineage Dynamics of Progenitors in the Human Cerebral Cortex
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151332
- ISBN
- 9798382814810
- DDC
- 574
- 서명/저자
- Mapping Lineage Dynamics of Progenitors in the Human Cerebral Cortex
- 발행사항
- [Sl] : University of California, San Francisco, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 156 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
- 주기사항
- Advisor: Wagner, Daniel.
- 학위논문주기
- Thesis (Ph.D.)--University of California, San Francisco, 2024.
- 초록/해제
- 요약During development of the cerebral cortex, progenitors give rise to diverse cell types in a spatially and temporally defined fashion, but the fate competence of individual progenitors and the lineage relationships between mature cell types has been difficult to ascertain. In this dissertation, I apply a novel prospective lineage tracing tool utilizing synthetic DNA barcodes to label human cortical progenitors and their resulting daughter cells in order to profile their output throughout neurogenesis. First, I show that cortical progenitors are capable to produce inhibitory interneurons, a cell type that had previously been widely believed to derive only from the ventral ganglionic eminences. In an in vitro model as well as in a xenotransplantation model, individual progenitors give rise to both excitatory and inhibitory neurons. Next, I extend this approach to more thoroughly analyze the temporal dynamics of cortical neurogenesis. Lineage tracing across the late second trimester reveals that inhibitory neurogenesis from dorsal progenitors is not a constant feature of cortical development, but arises prominently after midgestation. Furthermore, I show that excitatory neurogenesis proceeds at the ventricular zone throughout development, particularly by a subset of radial glia known as truncated radial glia. These excitatory neurons include neurons of deep layer identity, which are born throughout the second trimester, not just at early timepoints as had been previously believed. Together, these results shed light on previously unappreciated aspects of human cortical neurogenesis and highlight the diverse lineage potential of individual cortical progenitors.
- 일반주제명
- Developmental biology
- 일반주제명
- Neurosciences
- 일반주제명
- Cellular biology
- 키워드
- Cerebral cortex
- 키워드
- Lineage tracing
- 키워드
- Progenitors
- 키워드
- Radial glia
- 기타저자
- University of California, San Francisco Developmental and Stem Cell Biology
- 기본자료저록
- Dissertations Abstracts International. 85-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211151332
■006m o d
■007cr#unu||||||||
■020 ▼a9798382814810
■035 ▼a(MiAaPQ)AAI31241346
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aKeefe, Matthew George.▼0(orcid)0000-0002-2477-2975
■24510▼aMapping Lineage Dynamics of Progenitors in the Human Cerebral Cortex
■260 ▼a[Sl]▼bUniversity of California, San Francisco▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a156 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-12, Section: B.
■500 ▼aAdvisor: Wagner, Daniel.
■5021 ▼aThesis (Ph.D.)--University of California, San Francisco, 2024.
■520 ▼aDuring development of the cerebral cortex, progenitors give rise to diverse cell types in a spatially and temporally defined fashion, but the fate competence of individual progenitors and the lineage relationships between mature cell types has been difficult to ascertain. In this dissertation, I apply a novel prospective lineage tracing tool utilizing synthetic DNA barcodes to label human cortical progenitors and their resulting daughter cells in order to profile their output throughout neurogenesis. First, I show that cortical progenitors are capable to produce inhibitory interneurons, a cell type that had previously been widely believed to derive only from the ventral ganglionic eminences. In an in vitro model as well as in a xenotransplantation model, individual progenitors give rise to both excitatory and inhibitory neurons. Next, I extend this approach to more thoroughly analyze the temporal dynamics of cortical neurogenesis. Lineage tracing across the late second trimester reveals that inhibitory neurogenesis from dorsal progenitors is not a constant feature of cortical development, but arises prominently after midgestation. Furthermore, I show that excitatory neurogenesis proceeds at the ventricular zone throughout development, particularly by a subset of radial glia known as truncated radial glia. These excitatory neurons include neurons of deep layer identity, which are born throughout the second trimester, not just at early timepoints as had been previously believed. Together, these results shed light on previously unappreciated aspects of human cortical neurogenesis and highlight the diverse lineage potential of individual cortical progenitors.
■590 ▼aSchool code: 0034.
■650 4▼aDevelopmental biology
■650 4▼aNeurosciences
■650 4▼aCellular biology
■653 ▼aCerebral cortex
■653 ▼aCortical development
■653 ▼aLineage tracing
■653 ▼aProgenitors
■653 ▼aRadial glia
■690 ▼a0758
■690 ▼a0317
■690 ▼a0379
■71020▼aUniversity of California, San Francisco▼bDevelopmental and Stem Cell Biology.
■7730 ▼tDissertations Abstracts International▼g85-12B.
■790 ▼a0034
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17161271▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
