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Toward Systems Miniature Optical Microscopy: Instrumentation and Biomedical Applications
Toward Systems Miniature Optical Microscopy: Instrumentation and Biomedical Applications
Toward Systems Miniature Optical Microscopy: Instrumentation and Biomedical Applications

상세정보

자료유형  
 학위논문 서양
최종처리일시  
20260202105545
ISBN  
9798263395568
DDC  
001
저자명  
Son, Jeonghwan.
서명/저자  
Toward Systems Miniature Optical Microscopy: Instrumentation and Biomedical Applications
발행사항  
[Sl] : Georgia Institute of Technology, 2023
발행사항  
Ann Arbor : ProQuest Dissertations & Theses, 2023
형태사항  
176 p
주기사항  
Source: Dissertations Abstracts International, Volume: 87-05, Section: A.
주기사항  
Advisor: Jia, Shu.
학위논문주기  
Thesis (Ph.D.)--Georgia Institute of Technology, 2023.
초록/해제  
요약This thesis focuses on providing instrumentation and applications of optical microscopy with high portability, accessibility, and adaptable solution via miniature and open-source approaches. The thesis emphasizes the current challenges due to the limited adoption and utilization of recent fluorescence imaging systems, restricting the potential applications of advanced optical microscopy. We highlight the development of miniaturized microscopy (miniscopy) and its potential for broader applications. We introduce the background suppression and signal enhancement (BSSE) algorithm for enhancing imaging resolution of miniscopy and demonstrated the versatility of miniscopy in various ex vivo tissue imaging and in vivo neural activity processing. Next, we focus on developing systems for simultaneous in situ cell imaging using array miniscopy on multiwell plates. Miniaturized modular-array fluorescence microscopy (MAM) is a portable and flexible device that enables high-resolution fluorescence live-cell imaging. The system offers benefits in fluorescence imaging, flexibility, and scalability, making it suitable for various applications in bioimaging, clinical cell screening, and drug discovery. Lastly, we advance high-throughput imaging flow cytometry (IFC) through the implementation of portable light-sheet microscopy. A portable light-sheet optofluidic microscopy (PLSOM) is a miniature, high-resolution, multicolor 3D-IFC platform compatible with various microfluidic chips. It demonstrates its potential for high-throughput single-cell investigation and offers opportunities for broader flow cytometric studies. The work presented in this thesis lays the foundation for future advancement in fluorescence imaging, with potential impacts on various biomedical research endeavors.
일반주제명  
Software
일반주제명  
Investigations
일반주제명  
Brain cancer
일반주제명  
Semiconductors
일반주제명  
Cytotoxicity
일반주제명  
Brain research
일반주제명  
Systems design
일반주제명  
Labeling
일반주제명  
Light emitting diodes
일반주제명  
Biomedical research
일반주제명  
Neurosciences
일반주제명  
Flow cytometry
일반주제명  
Visualization
일반주제명  
Signal to noise ratio
일반주제명  
Neural networks
일반주제명  
Microscopy
일반주제명  
Flexibility
일반주제명  
Design
일반주제명  
Three dimensional imaging
일반주제명  
Tissues
일반주제명  
Cellular biology
일반주제명  
Electrical engineering
일반주제명  
Oncology
일반주제명  
Optics
기타저자  
Georgia Institute of Technology.
기본자료저록  
Dissertations Abstracts International. 87-05A.
전자적 위치 및 접속  
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MARC

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■1001  ▼aSon,  Jeonghwan.
■24510▼aToward  Systems  Miniature  Optical  Microscopy:  Instrumentation  and  Biomedical  Applications
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■260  1▼aAnn  Arbor▼bProQuest  Dissertations  &  Theses▼c2023
■300    ▼a176  p
■500    ▼aSource:  Dissertations  Abstracts  International,  Volume:  87-05,  Section:  A.
■500    ▼aAdvisor:  Jia,  Shu.
■5021  ▼aThesis  (Ph.D.)--Georgia  Institute  of  Technology,  2023.
■520    ▼aThis  thesis  focuses  on  providing  instrumentation  and  applications  of  optical  microscopy  with  high  portability,  accessibility,  and  adaptable  solution  via  miniature  and  open-source  approaches.  The  thesis  emphasizes  the  current  challenges  due  to  the  limited  adoption  and  utilization  of  recent  fluorescence  imaging  systems,  restricting  the  potential  applications  of  advanced  optical  microscopy.  We  highlight  the  development  of  miniaturized  microscopy  (miniscopy)  and  its  potential  for  broader  applications.  We  introduce  the  background  suppression  and  signal  enhancement  (BSSE)  algorithm  for  enhancing  imaging  resolution  of  miniscopy  and  demonstrated  the  versatility  of  miniscopy  in  various  ex  vivo  tissue  imaging  and  in  vivo  neural  activity  processing.  Next,  we  focus  on  developing  systems  for  simultaneous  in  situ  cell  imaging  using  array  miniscopy  on  multiwell  plates.  Miniaturized  modular-array  fluorescence  microscopy  (MAM)  is  a  portable  and  flexible  device  that  enables  high-resolution  fluorescence  live-cell  imaging.  The  system  offers  benefits  in  fluorescence  imaging,  flexibility,  and  scalability,  making  it  suitable  for  various  applications  in  bioimaging,  clinical  cell  screening,  and  drug  discovery.  Lastly,  we  advance  high-throughput  imaging  flow  cytometry  (IFC)  through  the  implementation  of  portable  light-sheet  microscopy.  A  portable  light-sheet  optofluidic  microscopy  (PLSOM)  is  a  miniature,  high-resolution,  multicolor  3D-IFC  platform  compatible  with  various  microfluidic  chips.  It  demonstrates  its  potential  for  high-throughput  single-cell  investigation  and  offers  opportunities  for  broader  flow  cytometric  studies.  The  work  presented  in  this  thesis  lays  the  foundation  for  future  advancement  in  fluorescence  imaging,  with  potential  impacts  on  various  biomedical  research  endeavors.
■590    ▼aSchool  code:  0078.
■650  4▼aSoftware
■650  4▼aInvestigations
■650  4▼aBrain  cancer
■650  4▼aSemiconductors
■650  4▼aCytotoxicity
■650  4▼aBrain  research
■650  4▼aSystems  design
■650  4▼aLabeling
■650  4▼aLight  emitting  diodes
■650  4▼aBiomedical  research
■650  4▼aNeurosciences
■650  4▼aFlow  cytometry
■650  4▼aVisualization
■650  4▼aSignal  to  noise  ratio
■650  4▼aNeural  networks
■650  4▼aMicroscopy
■650  4▼aFlexibility
■650  4▼aDesign
■650  4▼aThree  dimensional  imaging
■650  4▼aTissues
■650  4▼aCellular  biology
■650  4▼aElectrical  engineering
■650  4▼aOncology
■650  4▼aOptics
■690    ▼a0389
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■690    ▼a0800
■690    ▼a0379
■690    ▼a0544
■690    ▼a0992
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■71020▼aGeorgia  Institute  of  Technology.
■7730  ▼tDissertations  Abstracts  International▼g87-05A.
■790    ▼a0078
■791    ▼aPh.D.
■792    ▼a2023
■793    ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17360547▼nKERIS▼z이  자료의  원문은  한국교육학술정보원에서  제공합니다.

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