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Toward Systems Miniature Optical Microscopy: Instrumentation and Biomedical Applications
Toward Systems Miniature Optical Microscopy: Instrumentation and Biomedical Applications
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105545
- ISBN
- 9798263395568
- DDC
- 001
- 저자명
- Son, Jeonghwan.
- 서명/저자
- Toward Systems Miniature Optical Microscopy: Instrumentation and Biomedical Applications
- 발행사항
- [Sl] : Georgia Institute of Technology, 2023
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2023
- 형태사항
- 176 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-05, Section: A.
- 주기사항
- Advisor: Jia, Shu.
- 학위논문주기
- Thesis (Ph.D.)--Georgia Institute of Technology, 2023.
- 초록/해제
- 요약This thesis focuses on providing instrumentation and applications of optical microscopy with high portability, accessibility, and adaptable solution via miniature and open-source approaches. The thesis emphasizes the current challenges due to the limited adoption and utilization of recent fluorescence imaging systems, restricting the potential applications of advanced optical microscopy. We highlight the development of miniaturized microscopy (miniscopy) and its potential for broader applications. We introduce the background suppression and signal enhancement (BSSE) algorithm for enhancing imaging resolution of miniscopy and demonstrated the versatility of miniscopy in various ex vivo tissue imaging and in vivo neural activity processing. Next, we focus on developing systems for simultaneous in situ cell imaging using array miniscopy on multiwell plates. Miniaturized modular-array fluorescence microscopy (MAM) is a portable and flexible device that enables high-resolution fluorescence live-cell imaging. The system offers benefits in fluorescence imaging, flexibility, and scalability, making it suitable for various applications in bioimaging, clinical cell screening, and drug discovery. Lastly, we advance high-throughput imaging flow cytometry (IFC) through the implementation of portable light-sheet microscopy. A portable light-sheet optofluidic microscopy (PLSOM) is a miniature, high-resolution, multicolor 3D-IFC platform compatible with various microfluidic chips. It demonstrates its potential for high-throughput single-cell investigation and offers opportunities for broader flow cytometric studies. The work presented in this thesis lays the foundation for future advancement in fluorescence imaging, with potential impacts on various biomedical research endeavors.
- 일반주제명
- Software
- 일반주제명
- Investigations
- 일반주제명
- Brain cancer
- 일반주제명
- Semiconductors
- 일반주제명
- Cytotoxicity
- 일반주제명
- Brain research
- 일반주제명
- Systems design
- 일반주제명
- Labeling
- 일반주제명
- Light emitting diodes
- 일반주제명
- Biomedical research
- 일반주제명
- Neurosciences
- 일반주제명
- Flow cytometry
- 일반주제명
- Visualization
- 일반주제명
- Signal to noise ratio
- 일반주제명
- Neural networks
- 일반주제명
- Microscopy
- 일반주제명
- Flexibility
- 일반주제명
- Design
- 일반주제명
- Tissues
- 일반주제명
- Cellular biology
- 일반주제명
- Electrical engineering
- 일반주제명
- Oncology
- 일반주제명
- Optics
- 기본자료저록
- Dissertations Abstracts International. 87-05A.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798263395568
■035 ▼a(MiAaPQ)AAI32315522
■035 ▼a(MiAaPQ)GeorgiaTech73088
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a001
■1001 ▼aSon, Jeonghwan.
■24510▼aToward Systems Miniature Optical Microscopy: Instrumentation and Biomedical Applications
■260 ▼a[Sl]▼bGeorgia Institute of Technology▼c2023
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2023
■300 ▼a176 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-05, Section: A.
■500 ▼aAdvisor: Jia, Shu.
■5021 ▼aThesis (Ph.D.)--Georgia Institute of Technology, 2023.
■520 ▼aThis thesis focuses on providing instrumentation and applications of optical microscopy with high portability, accessibility, and adaptable solution via miniature and open-source approaches. The thesis emphasizes the current challenges due to the limited adoption and utilization of recent fluorescence imaging systems, restricting the potential applications of advanced optical microscopy. We highlight the development of miniaturized microscopy (miniscopy) and its potential for broader applications. We introduce the background suppression and signal enhancement (BSSE) algorithm for enhancing imaging resolution of miniscopy and demonstrated the versatility of miniscopy in various ex vivo tissue imaging and in vivo neural activity processing. Next, we focus on developing systems for simultaneous in situ cell imaging using array miniscopy on multiwell plates. Miniaturized modular-array fluorescence microscopy (MAM) is a portable and flexible device that enables high-resolution fluorescence live-cell imaging. The system offers benefits in fluorescence imaging, flexibility, and scalability, making it suitable for various applications in bioimaging, clinical cell screening, and drug discovery. Lastly, we advance high-throughput imaging flow cytometry (IFC) through the implementation of portable light-sheet microscopy. A portable light-sheet optofluidic microscopy (PLSOM) is a miniature, high-resolution, multicolor 3D-IFC platform compatible with various microfluidic chips. It demonstrates its potential for high-throughput single-cell investigation and offers opportunities for broader flow cytometric studies. The work presented in this thesis lays the foundation for future advancement in fluorescence imaging, with potential impacts on various biomedical research endeavors.
■590 ▼aSchool code: 0078.
■650 4▼aSoftware
■650 4▼aInvestigations
■650 4▼aBrain cancer
■650 4▼aSemiconductors
■650 4▼aCytotoxicity
■650 4▼aBrain research
■650 4▼aSystems design
■650 4▼aLabeling
■650 4▼aLight emitting diodes
■650 4▼aBiomedical research
■650 4▼aNeurosciences
■650 4▼aFlow cytometry
■650 4▼aVisualization
■650 4▼aSignal to noise ratio
■650 4▼aNeural networks
■650 4▼aMicroscopy
■650 4▼aFlexibility
■650 4▼aDesign
■650 4▼aThree dimensional imaging
■650 4▼aTissues
■650 4▼aCellular biology
■650 4▼aElectrical engineering
■650 4▼aOncology
■650 4▼aOptics
■690 ▼a0389
■690 ▼a0317
■690 ▼a0800
■690 ▼a0379
■690 ▼a0544
■690 ▼a0992
■690 ▼a0752
■71020▼aGeorgia Institute of Technology.
■7730 ▼tDissertations Abstracts International▼g87-05A.
■790 ▼a0078
■791 ▼aPh.D.
■792 ▼a2023
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17360547▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.


