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High-Aspect-Ratio Metal-on-Dielectric Nanostructures for Infrared Cellular Spectroscopy
High-Aspect-Ratio Metal-on-Dielectric Nanostructures for Infrared Cellular Spectroscopy
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202102954
- ISBN
- 9798283138169
- DDC
- 530
- 서명/저자
- High-Aspect-Ratio Metal-on-Dielectric Nanostructures for Infrared Cellular Spectroscopy
- 발행사항
- [Sl] : Cornell University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 129 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
- 주기사항
- Advisor: Shvets, Gennady.
- 학위논문주기
- Thesis (Ph.D.)--Cornell University, 2025.
- 초록/해제
- 요약Fourier Transform Infrared spectroscopy (FTIR) provides unique information about the composition and dynamics of biochemical systems by resolving the characteristic absorption fingerprint of the constituent biomolecules. However, for biological objects such as cells transmission-based FTIR is prevented by strong water absorption of mid-infrared (MIR) light. One of the popular measurement methods to get around this issue is growing cells on plasmonic metasurfaces with strong reflection in the spectral range intended to be probed by infrared spectroscopy. While the characteristic nearfields of the plasmonic resonators, which decay over couple hundreds of nanometers in depth, help mitigate water absorption issue this also leads to a shallow probing volume localized to the plasma membrane of the cells. Inspired by the recent introduction of high-aspect-ratio nanostructures as a novel platform for manipulating cellular behavior, we demonstrate the integration of metallic nanostructures with tall dielectric nanopillars to improve the sensing capabilities of FTIR.In the first chapter, we integrated the elevated dielectric pillars with plasmonic nanostructures as a method to improve the limitation of shallow probing depth of plasmonic resonators. Compared to flat 2D nanoantenna metasurfaces these raised 3D nanoantenna metasurfaces achieved about 1.8 times increase in sensitivity in the Amide spectral region. We also demonstrated the ability of these metal-on-dielectric metasurface to transduce intracellular processes such as protein translocation associated with cell endocytosis into interpretable spectral signatures of the reflected light.In the second chapter, we introduce a broadband biosensing platform based on metallic nanograting fabricated atop elevated dielectric pillars. For wavelengths significantly longer than the grating period, reflection-based spectroscopy enables sensing of the analytes inside the trenches separating the dielectric pillars. Because light traverses the analyte length twice this phenomenon is referred to as transflection. Along with the ability to sense chemical fingerprint of analytes over a wide range of 1400 - 3000 cm-1, we demonstrated the ability to characterize cells that naturally wrap themselves around the grating via the cellular process of endocytosis. We also show that with a tall enough (~1.5 μm) dielectric pillar we can achieve sensitivity similar to that of high-index ATR (Attenuated Total Reflection) prisms.In the third chapter, we discuss materials and device design to make the fabrication of these metal-on-dielectric metasurface devices foundry compatible. While optical metasurfaces have been demonstrated as a promising platform for biosensing, especially live-cell IR spectroscopy, and can be integrated into standard cell culture workflows using, practical commercial deployment is currently hindered by the lack of scalable, low-cost, and high throughput fabrication methods. The main limitation being the conventional MIR substrates like fluoride-based materials, which are brittle and thermally unstable. To address this limitation, we developed a novel fabrication process using a thin film dielectric free-standing membrane with high MIR transmission as a substrate. This method should enable mass production of metasurface based devices and facilitate the commercialization of microwell based measurement platform for real-time, label-free cellular infrared spectroscopy.
- 일반주제명
- Applied physics
- 일반주제명
- Cellular biology
- 일반주제명
- Physics
- 일반주제명
- Nanotechnology
- 키워드
- Mid-infrared
- 키워드
- Nanostructures
- 기타저자
- Cornell University Applied Physics
- 기본자료저록
- Dissertations Abstracts International. 86-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■006m o d
■007cr#unu||||||||
■020 ▼a9798283138169
■035 ▼a(MiAaPQ)AAI31768395
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a530
■1001 ▼aMahalanabish, Aditya.▼0(orcid)0000-0002-9489-4863
■24510▼aHigh-Aspect-Ratio Metal-on-Dielectric Nanostructures for Infrared Cellular Spectroscopy
■260 ▼a[Sl]▼bCornell University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a129 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-12, Section: B.
■500 ▼aAdvisor: Shvets, Gennady.
■5021 ▼aThesis (Ph.D.)--Cornell University, 2025.
■520 ▼aFourier Transform Infrared spectroscopy (FTIR) provides unique information about the composition and dynamics of biochemical systems by resolving the characteristic absorption fingerprint of the constituent biomolecules. However, for biological objects such as cells transmission-based FTIR is prevented by strong water absorption of mid-infrared (MIR) light. One of the popular measurement methods to get around this issue is growing cells on plasmonic metasurfaces with strong reflection in the spectral range intended to be probed by infrared spectroscopy. While the characteristic nearfields of the plasmonic resonators, which decay over couple hundreds of nanometers in depth, help mitigate water absorption issue this also leads to a shallow probing volume localized to the plasma membrane of the cells. Inspired by the recent introduction of high-aspect-ratio nanostructures as a novel platform for manipulating cellular behavior, we demonstrate the integration of metallic nanostructures with tall dielectric nanopillars to improve the sensing capabilities of FTIR.In the first chapter, we integrated the elevated dielectric pillars with plasmonic nanostructures as a method to improve the limitation of shallow probing depth of plasmonic resonators. Compared to flat 2D nanoantenna metasurfaces these raised 3D nanoantenna metasurfaces achieved about 1.8 times increase in sensitivity in the Amide spectral region. We also demonstrated the ability of these metal-on-dielectric metasurface to transduce intracellular processes such as protein translocation associated with cell endocytosis into interpretable spectral signatures of the reflected light.In the second chapter, we introduce a broadband biosensing platform based on metallic nanograting fabricated atop elevated dielectric pillars. For wavelengths significantly longer than the grating period, reflection-based spectroscopy enables sensing of the analytes inside the trenches separating the dielectric pillars. Because light traverses the analyte length twice this phenomenon is referred to as transflection. Along with the ability to sense chemical fingerprint of analytes over a wide range of 1400 - 3000 cm-1, we demonstrated the ability to characterize cells that naturally wrap themselves around the grating via the cellular process of endocytosis. We also show that with a tall enough (~1.5 μm) dielectric pillar we can achieve sensitivity similar to that of high-index ATR (Attenuated Total Reflection) prisms.In the third chapter, we discuss materials and device design to make the fabrication of these metal-on-dielectric metasurface devices foundry compatible. While optical metasurfaces have been demonstrated as a promising platform for biosensing, especially live-cell IR spectroscopy, and can be integrated into standard cell culture workflows using, practical commercial deployment is currently hindered by the lack of scalable, low-cost, and high throughput fabrication methods. The main limitation being the conventional MIR substrates like fluoride-based materials, which are brittle and thermally unstable. To address this limitation, we developed a novel fabrication process using a thin film dielectric free-standing membrane with high MIR transmission as a substrate. This method should enable mass production of metasurface based devices and facilitate the commercialization of microwell based measurement platform for real-time, label-free cellular infrared spectroscopy.
■590 ▼aSchool code: 0058.
■650 4▼aApplied physics
■650 4▼aCellular biology
■650 4▼aPhysics
■650 4▼aNanotechnology
■653 ▼aFourier Transform Infrared spectroscopy
■653 ▼aMid-infrared
■653 ▼aMetal-on-dielectric metasurface
■653 ▼aNanostructures
■653 ▼aCellular infrared spectroscopy
■690 ▼a0215
■690 ▼a0379
■690 ▼a0652
■690 ▼a0605
■71020▼aCornell University▼bApplied Physics.
■7730 ▼tDissertations Abstracts International▼g86-12B.
■790 ▼a0058
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17356568▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
