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Evaluating the Influence of Endophytic Microorganisms on the Success of Two Vegetatively Propagated Crops: Potato and Cranberry
Evaluating the Influence of Endophytic Microorganisms on the Success of Two Vegetatively P...
Evaluating the Influence of Endophytic Microorganisms on the Success of Two Vegetatively Propagated Crops: Potato and Cranberry

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자료유형  
 학위논문 서양
최종처리일시  
20260202104647
ISBN  
9798280755024
DDC  
581
저자명  
Vazquez-Catoni, Ana M.
서명/저자  
Evaluating the Influence of Endophytic Microorganisms on the Success of Two Vegetatively Propagated Crops: Potato and Cranberry
발행사항  
[Sl] : The University of Wisconsin - Madison, 2025
발행사항  
Ann Arbor : ProQuest Dissertations & Theses, 2025
형태사항  
182 p
주기사항  
Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
주기사항  
Advisor: Holland, Leslie A.
학위논문주기  
Thesis (Ph.D.)--The University of Wisconsin - Madison, 2025.
초록/해제  
요약Vegetative propagation methods are essential for producing high-quality planting material, though they present varying risks for microbial transmission. Certification programs aim to ensure disease-free stock, yet economic and regulatory disparities lead to inconsistent implementation across crops. Much remains unknown about how propagation systems and on-farm practices influence microbial communities. This dissertation identifies factors shaping endophytic microorganisms in two vegetatively propagated crops, potato and cranberry, through a series of integrated studies. In seed potato systems, a 2:1 perlite and calcined clay substrate produced high-quality tubers comparable to peat-based media, with no significant differences in endophytic bacterial diversity, supporting its use as a viable alternative. In cranberry, a grower survey revealed widespread concerns about disease transmission through vegetative cuttings, with regional differences in disease prevalence underscoring the need for standardized risk assessment protocols. Follow-up culture- and sequence-based analyses identified seven fungal genera associated with asymptomatic planting material, including key cranberry fruit rot (CFR) pathogens such as Phyllosticta, Phomopsis, and Godronia. These pathogens varied by tissue type and season, with notable year-to-year shifts, suggesting that planting material may contribute to CFR establishment in new beds. Finally, phylogenetic analysis of Phyllosticta spp. revealed that most isolates clustered with P. elongata, while a distinct lineage of P. vaccinii, was also detected. Fungicide sensitivity assays showed strong inhibition by commonly used active ingredients, though slightly reduced sensitivity to prothioconazole at low concentrations was observed. Together, these findings offer critical insights into microbial dynamics during early propagation and highlight practical areas for disease risk mitigation, substrate optimization, and resistance monitoring in potato and cranberry production systems.
일반주제명  
Plant pathology
일반주제명  
Agriculture
일반주제명  
Microbiology
일반주제명  
Food science
키워드  
Cranberry
키워드  
Endophytes
키워드  
Microbial transmission
키워드  
Phylogenetic analysis
키워드  
Potato
키워드  
Vegetative propagation
기타저자  
The University of Wisconsin - Madison Plant Pathology
기본자료저록  
Dissertations Abstracts International. 86-12B.
전자적 위치 및 접속  
로그인 후 원문을 볼 수 있습니다.

MARC

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■035    ▼a(MiAaPQ)AAI32114728
■040    ▼aMiAaPQ▼cMiAaPQ
■0820  ▼a581
■1001  ▼aVazquez-Catoni,  Ana  M.
■24510▼aEvaluating  the  Influence  of  Endophytic  Microorganisms  on  the  Success  of  Two  Vegetatively  Propagated  Crops:  Potato  and  Cranberry
■260    ▼a[Sl]▼bThe  University  of  Wisconsin  -  Madison▼c2025
■260  1▼aAnn  Arbor▼bProQuest  Dissertations  &  Theses▼c2025
■300    ▼a182  p
■500    ▼aSource:  Dissertations  Abstracts  International,  Volume:  86-12,  Section:  B.
■500    ▼aAdvisor:  Holland,  Leslie  A.
■5021  ▼aThesis  (Ph.D.)--The  University  of  Wisconsin  -  Madison,  2025.
■520    ▼aVegetative  propagation  methods  are  essential  for  producing  high-quality  planting  material,  though  they  present  varying  risks  for  microbial  transmission.  Certification  programs  aim  to  ensure  disease-free  stock,  yet  economic  and  regulatory  disparities  lead  to  inconsistent  implementation  across  crops.  Much  remains  unknown  about  how  propagation  systems  and  on-farm  practices  influence  microbial  communities.  This  dissertation  identifies  factors  shaping  endophytic  microorganisms  in  two  vegetatively  propagated  crops,  potato  and  cranberry,  through  a  series  of  integrated  studies.  In  seed  potato  systems,  a  2:1  perlite  and  calcined  clay  substrate  produced  high-quality  tubers  comparable  to  peat-based  media,  with  no  significant  differences  in  endophytic  bacterial  diversity,  supporting  its  use  as  a  viable  alternative.  In  cranberry,  a  grower  survey  revealed  widespread  concerns  about  disease  transmission  through  vegetative  cuttings,  with  regional  differences  in  disease  prevalence  underscoring  the  need  for  standardized  risk  assessment  protocols.  Follow-up  culture-  and  sequence-based  analyses  identified  seven  fungal  genera  associated  with  asymptomatic  planting  material,  including  key  cranberry  fruit  rot  (CFR)  pathogens  such  as  Phyllosticta,  Phomopsis,  and  Godronia.  These  pathogens  varied  by  tissue  type  and  season,  with  notable  year-to-year  shifts,  suggesting  that  planting  material  may  contribute  to  CFR  establishment  in  new  beds.  Finally,  phylogenetic  analysis  of  Phyllosticta  spp.  revealed  that  most  isolates  clustered  with  P.  elongata,  while  a  distinct  lineage  of  P.  vaccinii,  was  also  detected.  Fungicide  sensitivity  assays  showed  strong  inhibition  by  commonly  used  active  ingredients,  though  slightly  reduced  sensitivity  to  prothioconazole  at  low  concentrations  was  observed.  Together,  these  findings  offer  critical  insights  into  microbial  dynamics  during  early  propagation  and  highlight  practical  areas  for  disease  risk  mitigation,  substrate  optimization,  and  resistance  monitoring  in  potato  and  cranberry  production  systems.
■590    ▼aSchool  code:  0262.
■650  4▼aPlant  pathology
■650  4▼aAgriculture
■650  4▼aMicrobiology
■650  4▼aFood  science
■653    ▼aCranberry
■653    ▼aEndophytes
■653    ▼aMicrobial  transmission
■653    ▼aPhylogenetic  analysis
■653    ▼aPotato
■653    ▼aVegetative  propagation
■690    ▼a0480
■690    ▼a0473
■690    ▼a0410
■690    ▼a0359
■71020▼aThe  University  of  Wisconsin  -  Madison▼bPlant  Pathology.
■7730  ▼tDissertations  Abstracts  International▼g86-12B.
■790    ▼a0262
■791    ▼aPh.D.
■792    ▼a2025
■793    ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17358345▼nKERIS▼z이  자료의  원문은  한국교육학술정보원에서  제공합니다.

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