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Evaluating the Influence of Endophytic Microorganisms on the Success of Two Vegetatively Propagated Crops: Potato and Cranberry
Evaluating the Influence of Endophytic Microorganisms on the Success of Two Vegetatively Propagated Crops: Potato and Cranberry
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202104647
- ISBN
- 9798280755024
- DDC
- 581
- 서명/저자
- Evaluating the Influence of Endophytic Microorganisms on the Success of Two Vegetatively Propagated Crops: Potato and Cranberry
- 발행사항
- [Sl] : The University of Wisconsin - Madison, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 182 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
- 주기사항
- Advisor: Holland, Leslie A.
- 학위논문주기
- Thesis (Ph.D.)--The University of Wisconsin - Madison, 2025.
- 초록/해제
- 요약Vegetative propagation methods are essential for producing high-quality planting material, though they present varying risks for microbial transmission. Certification programs aim to ensure disease-free stock, yet economic and regulatory disparities lead to inconsistent implementation across crops. Much remains unknown about how propagation systems and on-farm practices influence microbial communities. This dissertation identifies factors shaping endophytic microorganisms in two vegetatively propagated crops, potato and cranberry, through a series of integrated studies. In seed potato systems, a 2:1 perlite and calcined clay substrate produced high-quality tubers comparable to peat-based media, with no significant differences in endophytic bacterial diversity, supporting its use as a viable alternative. In cranberry, a grower survey revealed widespread concerns about disease transmission through vegetative cuttings, with regional differences in disease prevalence underscoring the need for standardized risk assessment protocols. Follow-up culture- and sequence-based analyses identified seven fungal genera associated with asymptomatic planting material, including key cranberry fruit rot (CFR) pathogens such as Phyllosticta, Phomopsis, and Godronia. These pathogens varied by tissue type and season, with notable year-to-year shifts, suggesting that planting material may contribute to CFR establishment in new beds. Finally, phylogenetic analysis of Phyllosticta spp. revealed that most isolates clustered with P. elongata, while a distinct lineage of P. vaccinii, was also detected. Fungicide sensitivity assays showed strong inhibition by commonly used active ingredients, though slightly reduced sensitivity to prothioconazole at low concentrations was observed. Together, these findings offer critical insights into microbial dynamics during early propagation and highlight practical areas for disease risk mitigation, substrate optimization, and resistance monitoring in potato and cranberry production systems.
- 일반주제명
- Plant pathology
- 일반주제명
- Agriculture
- 일반주제명
- Microbiology
- 일반주제명
- Food science
- 키워드
- Cranberry
- 키워드
- Endophytes
- 키워드
- Potato
- 기타저자
- The University of Wisconsin - Madison Plant Pathology
- 기본자료저록
- Dissertations Abstracts International. 86-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520260202104647
■006m o d
■007cr#unu||||||||
■020 ▼a9798280755024
■035 ▼a(MiAaPQ)AAI32114728
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a581
■1001 ▼aVazquez-Catoni, Ana M.
■24510▼aEvaluating the Influence of Endophytic Microorganisms on the Success of Two Vegetatively Propagated Crops: Potato and Cranberry
■260 ▼a[Sl]▼bThe University of Wisconsin - Madison▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a182 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-12, Section: B.
■500 ▼aAdvisor: Holland, Leslie A.
■5021 ▼aThesis (Ph.D.)--The University of Wisconsin - Madison, 2025.
■520 ▼aVegetative propagation methods are essential for producing high-quality planting material, though they present varying risks for microbial transmission. Certification programs aim to ensure disease-free stock, yet economic and regulatory disparities lead to inconsistent implementation across crops. Much remains unknown about how propagation systems and on-farm practices influence microbial communities. This dissertation identifies factors shaping endophytic microorganisms in two vegetatively propagated crops, potato and cranberry, through a series of integrated studies. In seed potato systems, a 2:1 perlite and calcined clay substrate produced high-quality tubers comparable to peat-based media, with no significant differences in endophytic bacterial diversity, supporting its use as a viable alternative. In cranberry, a grower survey revealed widespread concerns about disease transmission through vegetative cuttings, with regional differences in disease prevalence underscoring the need for standardized risk assessment protocols. Follow-up culture- and sequence-based analyses identified seven fungal genera associated with asymptomatic planting material, including key cranberry fruit rot (CFR) pathogens such as Phyllosticta, Phomopsis, and Godronia. These pathogens varied by tissue type and season, with notable year-to-year shifts, suggesting that planting material may contribute to CFR establishment in new beds. Finally, phylogenetic analysis of Phyllosticta spp. revealed that most isolates clustered with P. elongata, while a distinct lineage of P. vaccinii, was also detected. Fungicide sensitivity assays showed strong inhibition by commonly used active ingredients, though slightly reduced sensitivity to prothioconazole at low concentrations was observed. Together, these findings offer critical insights into microbial dynamics during early propagation and highlight practical areas for disease risk mitigation, substrate optimization, and resistance monitoring in potato and cranberry production systems.
■590 ▼aSchool code: 0262.
■650 4▼aPlant pathology
■650 4▼aAgriculture
■650 4▼aMicrobiology
■650 4▼aFood science
■653 ▼aCranberry
■653 ▼aEndophytes
■653 ▼aMicrobial transmission
■653 ▼aPhylogenetic analysis
■653 ▼aPotato
■653 ▼aVegetative propagation
■690 ▼a0480
■690 ▼a0473
■690 ▼a0410
■690 ▼a0359
■71020▼aThe University of Wisconsin - Madison▼bPlant Pathology.
■7730 ▼tDissertations Abstracts International▼g86-12B.
■790 ▼a0262
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17358345▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.


