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Characterization of a CYP81A in Triticum aestivum L. That Endows Natural Herbicide Tolerance
Characterization of a CYP81A in Triticum aestivum L. That Endows Natural Herbicide Tolerance
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105209
- ISBN
- 9798291563809
- DDC
- 630
- 서명/저자
- Characterization of a CYP81A in Triticum aestivum L. That Endows Natural Herbicide Tolerance
- 발행사항
- [Sl] : University of Illinois at Urbana-Champaign, 2023
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2023
- 형태사항
- 163 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-02, Section: B.
- 주기사항
- Advisor: Riechers, Dean E.
- 학위논문주기
- Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2023.
- 초록/해제
- 요약Allohexaploid bread wheat (Triticum aestivum L.) is one of the most widely cultivated crops globally and possesses natural tolerance to certain herbicides through rapid detoxification. Herbicide safeners, such as cloquintocet-mexyl (CM), are often applied in wheat to increase the expression of the genes encoding herbicide-detoxifying enzymes, which results in enhanced herbicide tolerance. One common wheat herbicide utilized with CM is the synthetic auxin, halauxifen-methyl (HM). Wheat esterases de-esterify HM to the biologically active form, halauxifen acid (HA), then cytochrome P450 monooxygenases (CYPs) rapidly detoxify HA to non-phytotoxic, polar metabolites. While the HA detoxification pathway in wheat has been established, the genes encoding the enzymes that catalyze these reactions have not been identified. Previous research utilizing wheat alien substitution and nullisomic-tetrasomic (NT) lines identified plants lacking chromosome 5A exhibited increased sensitivity to HM, indicating genes necessary for HA tolerance are located on this chromosome. To further characterize these lines, LC-MS is necessary to confirm the increased injury from HM is due to reduced rates or different pathways of HA detoxification. Furthermore, candidate genes can be identified and validated through molecular and phenotypic experimentation.Chapter 1 includes a literature review of CYP81A genes in wheat and other grass species, a description of clustered regularly interspaced short palindromic repeats (CRISPR)-editing, and the recent advances of CRISPR-editing in wheat. Chapter 2 describes LC-MS experiments to measure HA levels in the alien substitution and NT lines that previously displayed variable HM tolerance in greenhouse experimentation. These results indicate wheat lines lacking chromosome 5A have a reduced ability to detoxify HA, while lines possessing wheat chromosome 5A maintain the ability to detoxify HA. Experiments in Chapter 3 allowed for identification of a candidate CYP81A gene located on chromosome 5A (denoted as CYP81A-5A) and characterized CYP81A-5A as CM inducible but not HM inducible. Chapter 4 describes CRISPR-editing of CYP81A-5A to create progeny with a mutation in the promoter region. This mutant allele was inherited in a Mendelian fashion and had no effect on several developmental traits. However, progeny homozygous for the mutant allele displayed an increase in expression following CM treatment and increased tolerance to herbicide treatment relative to unedited wheat.
- 일반주제명
- Agronomy
- 일반주제명
- Molecular biology
- 일반주제명
- Agriculture
- 일반주제명
- Agricultural chemistry
- 일반주제명
- Genetics
- 키워드
- Cytochrome p450
- 키워드
- Synthetic auxin
- 기타저자
- University of Illinois at Urbana-Champaign Crop Sciences
- 기본자료저록
- Dissertations Abstracts International. 87-02B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■1001 ▼aLandau, Olivia Augusta.
■24510▼aCharacterization of a CYP81A in Triticum aestivum L. That Endows Natural Herbicide Tolerance
■260 ▼a[Sl]▼bUniversity of Illinois at Urbana-Champaign▼c2023
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2023
■300 ▼a163 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-02, Section: B.
■500 ▼aAdvisor: Riechers, Dean E.
■5021 ▼aThesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2023.
■520 ▼aAllohexaploid bread wheat (Triticum aestivum L.) is one of the most widely cultivated crops globally and possesses natural tolerance to certain herbicides through rapid detoxification. Herbicide safeners, such as cloquintocet-mexyl (CM), are often applied in wheat to increase the expression of the genes encoding herbicide-detoxifying enzymes, which results in enhanced herbicide tolerance. One common wheat herbicide utilized with CM is the synthetic auxin, halauxifen-methyl (HM). Wheat esterases de-esterify HM to the biologically active form, halauxifen acid (HA), then cytochrome P450 monooxygenases (CYPs) rapidly detoxify HA to non-phytotoxic, polar metabolites. While the HA detoxification pathway in wheat has been established, the genes encoding the enzymes that catalyze these reactions have not been identified. Previous research utilizing wheat alien substitution and nullisomic-tetrasomic (NT) lines identified plants lacking chromosome 5A exhibited increased sensitivity to HM, indicating genes necessary for HA tolerance are located on this chromosome. To further characterize these lines, LC-MS is necessary to confirm the increased injury from HM is due to reduced rates or different pathways of HA detoxification. Furthermore, candidate genes can be identified and validated through molecular and phenotypic experimentation.Chapter 1 includes a literature review of CYP81A genes in wheat and other grass species, a description of clustered regularly interspaced short palindromic repeats (CRISPR)-editing, and the recent advances of CRISPR-editing in wheat. Chapter 2 describes LC-MS experiments to measure HA levels in the alien substitution and NT lines that previously displayed variable HM tolerance in greenhouse experimentation. These results indicate wheat lines lacking chromosome 5A have a reduced ability to detoxify HA, while lines possessing wheat chromosome 5A maintain the ability to detoxify HA. Experiments in Chapter 3 allowed for identification of a candidate CYP81A gene located on chromosome 5A (denoted as CYP81A-5A) and characterized CYP81A-5A as CM inducible but not HM inducible. Chapter 4 describes CRISPR-editing of CYP81A-5A to create progeny with a mutation in the promoter region. This mutant allele was inherited in a Mendelian fashion and had no effect on several developmental traits. However, progeny homozygous for the mutant allele displayed an increase in expression following CM treatment and increased tolerance to herbicide treatment relative to unedited wheat.
■590 ▼aSchool code: 0090.
■650 4▼aAgronomy
■650 4▼aMolecular biology
■650 4▼aAgriculture
■650 4▼aAgricultural chemistry
■650 4▼aGenetics
■653 ▼aTriticum aestivum
■653 ▼aCytochrome p450
■653 ▼aSynthetic auxin
■653 ▼aHerbicide metabolism
■653 ▼aHerbicide tolerance
■690 ▼a0285
■690 ▼a0307
■690 ▼a0369
■690 ▼a0749
■690 ▼a0473
■71020▼aUniversity of Illinois at Urbana-Champaign▼bCrop Sciences.
■7730 ▼tDissertations Abstracts International▼g87-02B.
■790 ▼a0090
■791 ▼aPh.D.
■792 ▼a2023
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17359763▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
