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Photoacoustic Imaging and Sensing in Applications of Angiogenesis Monitoring and Bone Assessment
Photoacoustic Imaging and Sensing in Applications of Angiogenesis Monitoring and Bone Assessment
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105214
- ISBN
- 9798291565421
- DDC
- 610
- 저자명
- Xu, Zhanpeng.
- 서명/저자
- Photoacoustic Imaging and Sensing in Applications of Angiogenesis Monitoring and Bone Assessment
- 발행사항
- [Sl] : University of Michigan, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 110 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-03, Section: B.
- 주기사항
- Advisor: Wang, Xueding.
- 학위논문주기
- Thesis (Ph.D.)--University of Michigan, 2025.
- 초록/해제
- 요약As an emerging technology, photoacoustic (PA) imaging combines the advantages of optical resolution and ultrasound penetration, providing high spatial resolution, moderate imaging depth, excellent imaging contrast and functional imaging capability. This dissertation explores two implementations of the PA technology, i.e., photoacoustic microscopy (PAM) and photoacoustic sensing (PAS), as well as their applications in high-resolution angiogenesis monitoring and deep-penetration bone health assessment, respectively.Two experimental setups, i.e., a multi-modality imaging system based on PAM, and a photoacoustic and quantitative ultrasound (PAQUS) combined device based on PAS, were designed and developed. The multi-modality imaging system, including the imaging modalities of PAM and optical coherence tomography (OCT), provided a comprehensive depiction of the microenvironment in vivo. The PAQUS device, with tunable laser from 690 to 950 nm, provided PA and QUS sensing signals from deep bone tissue in the calcaneus. This thesis conducts four studies to verify the capability of these platform.First, a murine intravital imaging window chamber was developed for longitudinal observation of the tissue and angiogenic microenvironment in vivo. Hydrogels, with and without growth factor loading, were implanted into the bodies of mice and served as the experimental and control groups, respectively. The progression of angiogenesis was observed by using PAM, and vascular morphology analysis was performed to see the difference between two groups.Second, inspired by the resolution and sensitivity of PAM, we longitudinally tracked angiogenesis in a biomaterial scaffold stimulated by cancer cells with different metastatic capabilities. Here, the microporous poly(ε-caprolactone) (PCL) scaffold, with excellent biocompatibility and stability, served as a synthetic metastatic niche for cancer cell migration and the base for imaging observation. Quantitative image analysis of vascular parameters, such as vessel area density (VAD), vessel mean tortuosity (VMT), and total vessel length (TVL) substantiated these observations, with significant differences in vascular metrics emerging as early as 8 days post tumor-inoculation in metastatic models. This study identifies the potential for longitudinal monitoring of vascular remodeling at a subcutaneous site for assessing metastatic progression in triple-negative breast cancer (TNBC).Third, we focused on the development of the first semi-anthropomorphic photoacoustic calcaneus phantom based on nano computed tomography (nano-CT) and stereolithography (SLA) 3D printing, to provide a stable and controllable calibration source for the PAQUS device. The calcaneus phantom, with similar anatomical, optical, and ultrasound properties as the real human calcaneus, was an effective source for development of novel bone assessment technology combining PA and QUS, and offered a comprehensive reference to investigate the PAS signal from the deep bone tissue.Finally, in vivo calcaneus bone assessments on 140 Caucasian women were conducted, covering the subjects aging from 20 to 79 years old. During each individual experiment, multiwavelength PAQUS measurements (690 to 950 nm, with an interval of 10 nm) were performed from lateral and medial directions on left and right foot, i.e., a total of 4 different measurements per subject. The PA signal generated from the calcaneus was extracted based on the time of arrival and was utilized for further analysis. Photoacoustic spectral analysis (PASA) based on single representative absorption wavelength was performed to analyze the microstructure of specific chemical materials, like mineral, blood and lipid. Correlation analysis was also performed among the microstructure-related parameter, ultrasound-related parameters and age.In conclusion, this work presented two implementations of the PA technology, and their respective applications in angiogenesis monitoring and bone health assessment. The experimental results demonstrated the performance and capability of the PA technology and respective system setups, which highlighted the strong potential and promise for biomedical and clinical translation.
- 일반주제명
- Biomedical engineering
- 일반주제명
- Engineering
- 일반주제명
- Optics
- 일반주제명
- Oncology
- 키워드
- Photonics
- 키워드
- Cancer detection
- 기타저자
- University of Michigan Biomedical Engineering
- 기본자료저록
- Dissertations Abstracts International. 87-03B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■006m o d
■007cr#unu||||||||
■020 ▼a9798291565421
■035 ▼a(MiAaPQ)AAI32271743
■035 ▼a(MiAaPQ)umichrackham006526
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a610
■1001 ▼aXu, Zhanpeng.
■24510▼aPhotoacoustic Imaging and Sensing in Applications of Angiogenesis Monitoring and Bone Assessment
■260 ▼a[Sl]▼bUniversity of Michigan▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a110 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-03, Section: B.
■500 ▼aAdvisor: Wang, Xueding.
■5021 ▼aThesis (Ph.D.)--University of Michigan, 2025.
■520 ▼aAs an emerging technology, photoacoustic (PA) imaging combines the advantages of optical resolution and ultrasound penetration, providing high spatial resolution, moderate imaging depth, excellent imaging contrast and functional imaging capability. This dissertation explores two implementations of the PA technology, i.e., photoacoustic microscopy (PAM) and photoacoustic sensing (PAS), as well as their applications in high-resolution angiogenesis monitoring and deep-penetration bone health assessment, respectively.Two experimental setups, i.e., a multi-modality imaging system based on PAM, and a photoacoustic and quantitative ultrasound (PAQUS) combined device based on PAS, were designed and developed. The multi-modality imaging system, including the imaging modalities of PAM and optical coherence tomography (OCT), provided a comprehensive depiction of the microenvironment in vivo. The PAQUS device, with tunable laser from 690 to 950 nm, provided PA and QUS sensing signals from deep bone tissue in the calcaneus. This thesis conducts four studies to verify the capability of these platform.First, a murine intravital imaging window chamber was developed for longitudinal observation of the tissue and angiogenic microenvironment in vivo. Hydrogels, with and without growth factor loading, were implanted into the bodies of mice and served as the experimental and control groups, respectively. The progression of angiogenesis was observed by using PAM, and vascular morphology analysis was performed to see the difference between two groups.Second, inspired by the resolution and sensitivity of PAM, we longitudinally tracked angiogenesis in a biomaterial scaffold stimulated by cancer cells with different metastatic capabilities. Here, the microporous poly(ε-caprolactone) (PCL) scaffold, with excellent biocompatibility and stability, served as a synthetic metastatic niche for cancer cell migration and the base for imaging observation. Quantitative image analysis of vascular parameters, such as vessel area density (VAD), vessel mean tortuosity (VMT), and total vessel length (TVL) substantiated these observations, with significant differences in vascular metrics emerging as early as 8 days post tumor-inoculation in metastatic models. This study identifies the potential for longitudinal monitoring of vascular remodeling at a subcutaneous site for assessing metastatic progression in triple-negative breast cancer (TNBC).Third, we focused on the development of the first semi-anthropomorphic photoacoustic calcaneus phantom based on nano computed tomography (nano-CT) and stereolithography (SLA) 3D printing, to provide a stable and controllable calibration source for the PAQUS device. The calcaneus phantom, with similar anatomical, optical, and ultrasound properties as the real human calcaneus, was an effective source for development of novel bone assessment technology combining PA and QUS, and offered a comprehensive reference to investigate the PAS signal from the deep bone tissue.Finally, in vivo calcaneus bone assessments on 140 Caucasian women were conducted, covering the subjects aging from 20 to 79 years old. During each individual experiment, multiwavelength PAQUS measurements (690 to 950 nm, with an interval of 10 nm) were performed from lateral and medial directions on left and right foot, i.e., a total of 4 different measurements per subject. The PA signal generated from the calcaneus was extracted based on the time of arrival and was utilized for further analysis. Photoacoustic spectral analysis (PASA) based on single representative absorption wavelength was performed to analyze the microstructure of specific chemical materials, like mineral, blood and lipid. Correlation analysis was also performed among the microstructure-related parameter, ultrasound-related parameters and age.In conclusion, this work presented two implementations of the PA technology, and their respective applications in angiogenesis monitoring and bone health assessment. The experimental results demonstrated the performance and capability of the PA technology and respective system setups, which highlighted the strong potential and promise for biomedical and clinical translation.
■590 ▼aSchool code: 0127.
■650 4▼aBiomedical engineering
■650 4▼aEngineering
■650 4▼aOptics
■650 4▼aOncology
■653 ▼aPhotoacoustic imaging
■653 ▼aMultimodality imaging
■653 ▼aPhotonics
■653 ▼aAngiogenesis monitoring
■653 ▼aBone health assessment
■653 ▼aCancer detection
■690 ▼a0541
■690 ▼a0537
■690 ▼a0752
■690 ▼a0992
■71020▼aUniversity of Michigan▼bBiomedical Engineering.
■7730 ▼tDissertations Abstracts International▼g87-03B.
■790 ▼a0127
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17359789▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
