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Electro-Optic Techniques for Nanosecond Imaging and Applications to Fluorescence Lifetime Microscopy
Electro-Optic Techniques for Nanosecond Imaging and Applications to Fluorescence Lifetime Microscopy
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105617
- ISBN
- 9798265429308
- DDC
- 620
- 저자명
- Bowman, Adam J.
- 서명/저자
- Electro-Optic Techniques for Nanosecond Imaging and Applications to Fluorescence Lifetime Microscopy
- 발행사항
- [Sl] : Stanford University, 2023
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2023
- 형태사항
- 192 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-06, Section: B.
- 주기사항
- Includes supplementary digital materials.
- 주기사항
- Advisor: Kasevich, Mark.
- 학위논문주기
- Thesis (Ph.D.)--Stanford University, 2023.
- 초록/해제
- 요약Fluorescence microscopy allows targeted visualization of biological matter across spatial and temporal scales. Most methods rely on measurements of image intensity that are taken with a standard camera sensor at low frame rates. The nanosecond excited state lifetime of a fluorescent probe also carries valuable information about the local environment which can be used to improve optical measurements, but it cannot be captured with common cameras. Current lifetime detectors are either too slow or too noisy for many applications. This thesis presents the development of nanosecond imaging optics and the electro-optic fluorescence lifetime imaging microscopy (EO-FLIM) method. Fast optical gating of a wide-field image is achieved using electro-optic crystals, allowing lifetimes to be measured on scientific camera sensors with high sensitivity. EO-FLIM improves photon throughput by several orders of magnitude over standard time-resolved detectors. Lifetime is estimated from a ratio of optical intensities, which increases the information content of the captured image while also rejecting intensity noise and motion artifacts. Several optical systems and technological improvements are presented which have enabled wide-field lifetime imaging of single fluorescent molecules, combination of lifetime imaging with super-resolution localization microscopy, and lifetime recording of neuron action potentials and sub-threshold voltage activity in vivo at kilohertz frame rates. Applications to light-sheet microscopy and time-of-flight imaging are also shown.
- 일반주제명
- Engineering
- 일반주제명
- Optics
- 일반주제명
- Nanoscience
- 기타저자
- Stanford University.
- 기본자료저록
- Dissertations Abstracts International. 87-06B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■006m o d
■007cr#unu||||||||
■020 ▼a9798265429308
■035 ▼a(MiAaPQ)AAI32316469
■035 ▼a(MiAaPQ)Stanfordvm973kq1178
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a620
■1001 ▼aBowman, Adam J.
■24510▼aElectro-Optic Techniques for Nanosecond Imaging and Applications to Fluorescence Lifetime Microscopy
■260 ▼a[Sl]▼bStanford University▼c2023
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2023
■300 ▼a192 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-06, Section: B.
■500 ▼aIncludes supplementary digital materials.
■500 ▼aAdvisor: Kasevich, Mark.
■5021 ▼aThesis (Ph.D.)--Stanford University, 2023.
■520 ▼aFluorescence microscopy allows targeted visualization of biological matter across spatial and temporal scales. Most methods rely on measurements of image intensity that are taken with a standard camera sensor at low frame rates. The nanosecond excited state lifetime of a fluorescent probe also carries valuable information about the local environment which can be used to improve optical measurements, but it cannot be captured with common cameras. Current lifetime detectors are either too slow or too noisy for many applications. This thesis presents the development of nanosecond imaging optics and the electro-optic fluorescence lifetime imaging microscopy (EO-FLIM) method. Fast optical gating of a wide-field image is achieved using electro-optic crystals, allowing lifetimes to be measured on scientific camera sensors with high sensitivity. EO-FLIM improves photon throughput by several orders of magnitude over standard time-resolved detectors. Lifetime is estimated from a ratio of optical intensities, which increases the information content of the captured image while also rejecting intensity noise and motion artifacts. Several optical systems and technological improvements are presented which have enabled wide-field lifetime imaging of single fluorescent molecules, combination of lifetime imaging with super-resolution localization microscopy, and lifetime recording of neuron action potentials and sub-threshold voltage activity in vivo at kilohertz frame rates. Applications to light-sheet microscopy and time-of-flight imaging are also shown.
■590 ▼aSchool code: 0212.
■650 4▼aEngineering
■650 4▼aOptics
■650 4▼aNanoscience
■653 ▼aFluorescence microscopy
■690 ▼a0752
■690 ▼a0565
■690 ▼a0537
■71020▼aStanford University.
■7730 ▼tDissertations Abstracts International▼g87-06B.
■790 ▼a0212
■791 ▼aPh.D.
■792 ▼a2023
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17360772▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
