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Mass Spectrometry-Based Proteomic Investigations Into Human Disease
Mass Spectrometry-Based Proteomic Investigations Into Human Disease
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202104658
- ISBN
- 9798280770874
- DDC
- 615
- 서명/저자
- Mass Spectrometry-Based Proteomic Investigations Into Human Disease
- 발행사항
- [Sl] : The University of Wisconsin - Madison, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 227 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
- 주기사항
- Advisor: Li, Lingjun;Ricke, William A.
- 학위논문주기
- Thesis (Ph.D.)--The University of Wisconsin - Madison, 2025.
- 초록/해제
- 요약Mass spectrometry (MS) has become a powerful tool for examining human disease, offering high sensitivity and throughput across a wide range of analyte classes. Global proteomic profiling, achieved by coupling of liquid chromatography (LC) with tandem MS scans, commonly referred to as LC-MS/MS, has become a powerful modality in disease investigation and biomarker discovery. This dissertation focuses on the application of LC-MS/MS proteomic strategies to various human disease states, both benign and malignant. A basic workflow for simultaneous profiling of intracellular and extracellular proteomes using in vitro models was developed to probe cellular communication in the context of disease. When examining changes in a model of prostate cancer with therapeutic intervention, a senescent-like phenotype was observed, indicating a potential therapeutic avenue for pharmaceutical development. Additional studies centered on the development of a processing pipeline that integrates mass spectrometry imaging (MSI) with complementary LC-MS/MS data to improve identification confidence in MSI data and enable untargeted spatial profiling. This platform, named MSIght, was subsequently utilized alongside serum profiling to investigate the effects of estrogen receptor alpha inhibition in mouse models of lower urinary tract dysfunction (LUTD) associated with benign prostatic hyperplasia (BPH). Finally, an analysis of glycosylation in neuronal proteins - and their corresponding alterations - was performed in mouse models of fragile X syndrome (FXS), with a particular focus on the enrichment of synaptic glycoproteome alterations. This dissertation demonstrates the broad utility of MS-based proteomic strategies across diverse disease states and sample types, while also identifying and uncovering potential therapeutic avenues in each application.
- 일반주제명
- Pharmaceutical sciences
- 일반주제명
- Analytical chemistry
- 키워드
- Proteomics
- 기타저자
- The University of Wisconsin - Madison Pharmaceutical Sciences
- 기본자료저록
- Dissertations Abstracts International. 86-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798280770874
■035 ▼a(MiAaPQ)AAI32115954
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a615
■1001 ▼aMiles, Hannah Nicole.
■24510▼aMass Spectrometry-Based Proteomic Investigations Into Human Disease
■260 ▼a[Sl]▼bThe University of Wisconsin - Madison▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a227 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-12, Section: B.
■500 ▼aAdvisor: Li, Lingjun;Ricke, William A.
■5021 ▼aThesis (Ph.D.)--The University of Wisconsin - Madison, 2025.
■520 ▼aMass spectrometry (MS) has become a powerful tool for examining human disease, offering high sensitivity and throughput across a wide range of analyte classes. Global proteomic profiling, achieved by coupling of liquid chromatography (LC) with tandem MS scans, commonly referred to as LC-MS/MS, has become a powerful modality in disease investigation and biomarker discovery. This dissertation focuses on the application of LC-MS/MS proteomic strategies to various human disease states, both benign and malignant. A basic workflow for simultaneous profiling of intracellular and extracellular proteomes using in vitro models was developed to probe cellular communication in the context of disease. When examining changes in a model of prostate cancer with therapeutic intervention, a senescent-like phenotype was observed, indicating a potential therapeutic avenue for pharmaceutical development. Additional studies centered on the development of a processing pipeline that integrates mass spectrometry imaging (MSI) with complementary LC-MS/MS data to improve identification confidence in MSI data and enable untargeted spatial profiling. This platform, named MSIght, was subsequently utilized alongside serum profiling to investigate the effects of estrogen receptor alpha inhibition in mouse models of lower urinary tract dysfunction (LUTD) associated with benign prostatic hyperplasia (BPH). Finally, an analysis of glycosylation in neuronal proteins - and their corresponding alterations - was performed in mouse models of fragile X syndrome (FXS), with a particular focus on the enrichment of synaptic glycoproteome alterations. This dissertation demonstrates the broad utility of MS-based proteomic strategies across diverse disease states and sample types, while also identifying and uncovering potential therapeutic avenues in each application.
■590 ▼aSchool code: 0262.
■650 4▼aPharmaceutical sciences
■650 4▼aAnalytical chemistry
■653 ▼aMass spectrometry
■653 ▼aProteomics
■653 ▼aLiquid chromatography
■653 ▼aExtracellular proteomes
■690 ▼a0572
■690 ▼a0486
■71020▼aThe University of Wisconsin - Madison▼bPharmaceutical Sciences.
■7730 ▼tDissertations Abstracts International▼g86-12B.
■790 ▼a0262
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17358411▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
