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Spatiotemporal Coordination of Proliferation and Patterning in Early Embryonic Development
Spatiotemporal Coordination of Proliferation and Patterning in Early Embryonic Development
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105237
- ISBN
- 9798291567951
- DDC
- 530
- 저자명
- Kadiyala, Usha.
- 서명/저자
- Spatiotemporal Coordination of Proliferation and Patterning in Early Embryonic Development
- 발행사항
- [Sl] : University of Michigan, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 145 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-02, Section: B.
- 주기사항
- Advisor: Yang, Qiong.
- 학위논문주기
- Thesis (Ph.D.)--University of Michigan, 2025.
- 초록/해제
- 요약A central challenge in developmental biology is understanding how embryos coordinate cellular behaviors across space and time to transform a single cell into a structured organism. This dissertation investigates how zebrafish embryos solve this challenge through context-dependent coupling of proliferation, patterning, and mechanics during two key developmental stages: somitogenesis and early morphogenesis. During somitogenesis, the presomitic mesoderm (PSM) simultaneously proliferates and periodically segments into somites. We show that this coordination arises from spatially patterned, bidirectional coupling between the segmentation clock and the cell cycle, mediated by Notch signaling and cyclin-dependent kinases. This creates distinct functional zones where weak coupling in the posterior progenitor zone enables rapid proliferation, while strong phase-locking in the central PSM coordinates divisions with pattern formation. This oscillator coupling maintains proper somite proportions and creates tissue organization from local molecular interactions. Early morphogenesis presents a parallel coordination challenge: how do proliferation, differentiation, and mechanical forces integrate to drive autonomous tissue shape changes? Here, using zebrafish embryonic explants (pescoids) as a minimal active matter system, we reveal how cellular aggregates undergo fluid-to-solid-like and wetting to dwetting, transitions on a glass substrate. We show that the wetting phase is driven by proliferation-induced extensile forces, while the dewetting phase is mediated by mesendoderm specification and myosin II dependent contractility. This morphogenetic switch is intrinsically programmed, and we show that spatial gradients of proliferation, differentiation, and contractility create feedback loops that drive coordinated tissue-scale shape changes. Together, these studies demonstrate how zebrafish embryos achieve developmental coordination through context-dependent coupling mechanisms operating from molecular interactions to tissue-scale forces.
- 일반주제명
- Physics
- 일반주제명
- Developmental biology
- 일반주제명
- Biophysics
- 일반주제명
- Cellular biology
- 키워드
- Embryos
- 기타저자
- University of Michigan Biophysics
- 기본자료저록
- Dissertations Abstracts International. 87-02B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
008260126s2025 us c eng d■001000017359930
■00520260202105237
■006m o d
■007cr#unu||||||||
■020 ▼a9798291567951
■035 ▼a(MiAaPQ)AAI32271964
■035 ▼a(MiAaPQ)umichrackham006503
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a530
■1001 ▼aKadiyala, Usha.
■24510▼aSpatiotemporal Coordination of Proliferation and Patterning in Early Embryonic Development
■260 ▼a[Sl]▼bUniversity of Michigan▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a145 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-02, Section: B.
■500 ▼aAdvisor: Yang, Qiong.
■5021 ▼aThesis (Ph.D.)--University of Michigan, 2025.
■520 ▼aA central challenge in developmental biology is understanding how embryos coordinate cellular behaviors across space and time to transform a single cell into a structured organism. This dissertation investigates how zebrafish embryos solve this challenge through context-dependent coupling of proliferation, patterning, and mechanics during two key developmental stages: somitogenesis and early morphogenesis. During somitogenesis, the presomitic mesoderm (PSM) simultaneously proliferates and periodically segments into somites. We show that this coordination arises from spatially patterned, bidirectional coupling between the segmentation clock and the cell cycle, mediated by Notch signaling and cyclin-dependent kinases. This creates distinct functional zones where weak coupling in the posterior progenitor zone enables rapid proliferation, while strong phase-locking in the central PSM coordinates divisions with pattern formation. This oscillator coupling maintains proper somite proportions and creates tissue organization from local molecular interactions. Early morphogenesis presents a parallel coordination challenge: how do proliferation, differentiation, and mechanical forces integrate to drive autonomous tissue shape changes? Here, using zebrafish embryonic explants (pescoids) as a minimal active matter system, we reveal how cellular aggregates undergo fluid-to-solid-like and wetting to dwetting, transitions on a glass substrate. We show that the wetting phase is driven by proliferation-induced extensile forces, while the dewetting phase is mediated by mesendoderm specification and myosin II dependent contractility. This morphogenetic switch is intrinsically programmed, and we show that spatial gradients of proliferation, differentiation, and contractility create feedback loops that drive coordinated tissue-scale shape changes. Together, these studies demonstrate how zebrafish embryos achieve developmental coordination through context-dependent coupling mechanisms operating from molecular interactions to tissue-scale forces.
■590 ▼aSchool code: 0127.
■650 4▼aPhysics
■650 4▼aDevelopmental biology
■650 4▼aBiophysics
■650 4▼aCellular biology
■653 ▼aBiological oscillators
■653 ▼aSegmentation clock
■653 ▼aDevelopmental patterning
■653 ▼aEmbryos
■653 ▼aPresomitic mesoderm
■690 ▼a0786
■690 ▼a0605
■690 ▼a0758
■690 ▼a0379
■71020▼aUniversity of Michigan▼bBiophysics.
■7730 ▼tDissertations Abstracts International▼g87-02B.
■790 ▼a0127
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17359930▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
