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NaP-TRAP Reveals 5'UTR-Mediated Translation Regulation During Zebrafish Development
NaP-TRAP Reveals 5'UTR-Mediated Translation Regulation During Zebrafish Development
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103018
- ISBN
- 9798286445516
- DDC
- 574
- 서명/저자
- NaP-TRAP Reveals 5UTR-Mediated Translation Regulation During Zebrafish Development
- 발행사항
- [Sl] : Yale University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 132 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
- 주기사항
- Advisor: Giraldez, Antonio J.
- 학위논문주기
- Thesis (Ph.D.)--Yale University, 2025.
- 초록/해제
- 요약The cis-regulatory elements encoded in an mRNA determine its stability and translational output. While there has been a considerable effort to understand the factors driving mRNA stability, the regulatory frameworks governing translational control remain more elusive. We have developed a novel massively parallel reporter assay (MPRA) to measure mRNA translation, named Nascent Peptide Translating Ribosome Affinity Purification (NaP-TRAP). NaP-TRAP measures translation in a frame-specific manner through the immunocapture of epitope tagged nascent peptides of reporter mRNAs. We benchmark NaP-TRAP to polysome profiling and use it to quantify Kozak strength and the regulatory landscapes of 5' UTRs in the developing zebrafish embryo and in human cells. Through this approach we identified general and developmentally dynamic cis-regulatory elements, as well as potential trans-acting proteins. We find that U-rich motifs are general enhancers, and upstream ORFs and GC-rich motifs are global repressors of translation. We also observe a translational switch during the maternal-to-zygotic transition, where C-rich motifs shift from repressors to prominent activators of translation. Conversely, we show that microRNA sites in the 5' UTR repress translation following the zygotic expression of miR-430. Together these results demonstrate that NaP-TRAP is a versatile, accessible, and powerful method to decode the regulatory functions of UTRs across different systems.
- 일반주제명
- Molecular biology
- 일반주제명
- Developmental biology
- 일반주제명
- Genetics
- 키워드
- 5' UTR
- 키워드
- RNA
- 키워드
- Translation
- 키워드
- Zebrafish
- 기타저자
- Yale University Genetics
- 기본자료저록
- Dissertations Abstracts International. 86-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■006m o d
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■020 ▼a9798286445516
■035 ▼a(MiAaPQ)AAI31844128
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aStrayer, Ethan C.
■24510▼aNaP-TRAP Reveals 5'UTR-Mediated Translation Regulation During Zebrafish Development
■260 ▼a[Sl]▼bYale University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a132 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-12, Section: B.
■500 ▼aAdvisor: Giraldez, Antonio J.
■5021 ▼aThesis (Ph.D.)--Yale University, 2025.
■520 ▼aThe cis-regulatory elements encoded in an mRNA determine its stability and translational output. While there has been a considerable effort to understand the factors driving mRNA stability, the regulatory frameworks governing translational control remain more elusive. We have developed a novel massively parallel reporter assay (MPRA) to measure mRNA translation, named Nascent Peptide Translating Ribosome Affinity Purification (NaP-TRAP). NaP-TRAP measures translation in a frame-specific manner through the immunocapture of epitope tagged nascent peptides of reporter mRNAs. We benchmark NaP-TRAP to polysome profiling and use it to quantify Kozak strength and the regulatory landscapes of 5' UTRs in the developing zebrafish embryo and in human cells. Through this approach we identified general and developmentally dynamic cis-regulatory elements, as well as potential trans-acting proteins. We find that U-rich motifs are general enhancers, and upstream ORFs and GC-rich motifs are global repressors of translation. We also observe a translational switch during the maternal-to-zygotic transition, where C-rich motifs shift from repressors to prominent activators of translation. Conversely, we show that microRNA sites in the 5' UTR repress translation following the zygotic expression of miR-430. Together these results demonstrate that NaP-TRAP is a versatile, accessible, and powerful method to decode the regulatory functions of UTRs across different systems.
■590 ▼aSchool code: 0265.
■650 4▼aMolecular biology
■650 4▼aDevelopmental biology
■650 4▼aGenetics
■653 ▼a5' UTR
■653 ▼aMassively parallel reporter assay
■653 ▼aRNA
■653 ▼aTranslation
■653 ▼aZebrafish
■690 ▼a0307
■690 ▼a0369
■690 ▼a0758
■71020▼aYale University▼bGenetics.
■7730 ▼tDissertations Abstracts International▼g86-12B.
■790 ▼a0265
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17356693▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
