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Untangling Spatial, Environmental, and Genetic Drivers of Foliar Endophyte Community Assembly
Untangling Spatial, Environmental, and Genetic Drivers of Foliar Endophyte Community Assembly
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105255
- ISBN
- 9798263319304
- DDC
- 574.5
- 저자명
- Mueller, Taz.
- 서명/저자
- Untangling Spatial, Environmental, and Genetic Drivers of Foliar Endophyte Community Assembly
- 발행사항
- [Sl] : University of Minnesota, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 133 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-05, Section: B.
- 주기사항
- Advisor: Moeller, David A.
- 학위논문주기
- Thesis (Ph.D.)--University of Minnesota, 2025.
- 초록/해제
- 요약Plants and their microbiomes form integrated systems where the boundaries between host and microbe are often blurred. Foliar fungal endophytes-fungi living asymptomatically inside healthy leaves-colonize virtually all terrestrial plants and can profoundly influence host performance through effects on stress tolerance, pathogen resistance, and resource acquisition. Despite their ubiquity and ecological importance, we lack fundamental understanding of what determines which fungi colonize which plants. The central challenge is that multiple processes simultaneously shape communities-dispersal moves microbes across landscapes, environmental conditions filter which taxa can persist, and host plants selectively permit or exclude colonizers based on their traits-but these processes operate at different spatial scales and interact in complex ways that are rarely studied together, particularly in natural field environments.Understanding microbial community assembly requires disentangling processes that operate hierarchically across biological scales. Regional dispersal patterns determine which taxa are present in a landscape, local environmental conditions filter this regional species pool, and host plants impose a final filter through their genetic makeup and expressed traits. However, testing this framework is challenging because spatial and environmental variation are often confounded, environmental conditions can alter host trait expression, and the specific plant traits mediating host genetic effects remain largely mysterious. While agricultural systems have provided valuable mechanistic insights, wild plant populations remain critically understudied, particularly through experimental approaches that can establish causation rather than correlation. I address these challenges through a novel ecological genetics approach that combines observational surveys with experimental manipulations of host origin and genotype in wild populations. Working with Clarkia xantiana, a wild annual wildflower native to California's Sierra Nevada foothills, I progressively narrow spatial scale while increasing experimental control. In Chapter I, I surveyed endophyte communities across 50 sites spanning 100 kilometers to quantify how community structure is associated with geographic distance, environmental factors, and host identity. I separated out these often-confounded factors by repeatedly co-sampling three herbaceous plant species across a topographically complex landscape where space and environment are largely decoupled. Our study reveals that community composition shows strong associations with spatial distance, while alpha diversity varies in relation to environmental gradients differently across host species. Within sites, host species harbor largely distinct communities, yet the specific taxa associated with each host vary inconsistently across locations. This chapter establishes that different assembly processes may affect different axes of community variation and that the strength of host associations is scale-dependent.In Chapter II, I implement a fully reciprocal transplant experiment across four sites, growing plants from four genetically differentiated source populations at all four sites in a factorial design. Despite clear fitness variation among populations, I find that site effects overwhelm source population influences on endophyte communities. Local environmental filtering and the available microbial species pool appear far more important than host population genetics, even though populations differ in traits potentially relevant to microbe interactions. This reveals that while host species identity matters, naturally occurring intraspecific genetic variation has minimal detectable effects under realistic field conditions.In Chapter III, I take an experimental ecological genetic approach, manipulating host genotype within a single site using recombinant inbred lines that segregate for leaf anthocyanin content. By creating genetic variation in this target trait that exceeds what is found in natural populations, I test mechanistic hypotheses about trait-mediated assembly. Growing 48 genetic lines under factorial water and fungicide treatments, I find that anthocyanin content strongly predicts both community diversity and composition, with high-anthocyanin plants supporting greater diversity while excluding potentially pathogenic taxa that dominate low-anthocyanin plants. This work demonstrates that host chemical traits can mechanistically filter fungal colonization, revealing that while naturally occurring population-level variation may be insufficient to structure communities (Chapter II), experimental manipulation of genetic variation exposes underlying trait-based mechanisms. This establishes that secondary metabolite variation represents a fundamental organizing principle in foliar microbiome assembly, with implications for understanding how plant chemical diversity shapes microbial communities and how these interactions might evolve or be manipulated. Taken together, this work establishes a hierarchical framework where regional dispersal patterns, local environmental filtering, and host chemical traits interact across scales to shape microbial community assembly in natural ecosystems. By revealing which processes operate at which scales and identifying specific mechanisms of host filtering through experimental manipulation in wild plant populations-an approach rarely applied to research on foliar endophytes-this dissertation provides a conceptual foundation for understanding the community assembly frameworks that shape plant microbiomes.
- 일반주제명
- Ecology
- 일반주제명
- Microbiology
- 일반주제명
- Botany
- 일반주제명
- Genetics
- 키워드
- Anthocyanins
- 키워드
- Genetic filters
- 키워드
- Plant microbiome
- 기타저자
- University of Minnesota Ecology Evolution and Behavior
- 기본자료저록
- Dissertations Abstracts International. 87-05B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■007cr#unu||||||||
■020 ▼a9798263319304
■035 ▼a(MiAaPQ)AAI32278386
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574.5
■1001 ▼aMueller, Taz.
■24510▼aUntangling Spatial, Environmental, and Genetic Drivers of Foliar Endophyte Community Assembly
■260 ▼a[Sl]▼bUniversity of Minnesota▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a133 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-05, Section: B.
■500 ▼aAdvisor: Moeller, David A.
■5021 ▼aThesis (Ph.D.)--University of Minnesota, 2025.
■520 ▼aPlants and their microbiomes form integrated systems where the boundaries between host and microbe are often blurred. Foliar fungal endophytes-fungi living asymptomatically inside healthy leaves-colonize virtually all terrestrial plants and can profoundly influence host performance through effects on stress tolerance, pathogen resistance, and resource acquisition. Despite their ubiquity and ecological importance, we lack fundamental understanding of what determines which fungi colonize which plants. The central challenge is that multiple processes simultaneously shape communities-dispersal moves microbes across landscapes, environmental conditions filter which taxa can persist, and host plants selectively permit or exclude colonizers based on their traits-but these processes operate at different spatial scales and interact in complex ways that are rarely studied together, particularly in natural field environments.Understanding microbial community assembly requires disentangling processes that operate hierarchically across biological scales. Regional dispersal patterns determine which taxa are present in a landscape, local environmental conditions filter this regional species pool, and host plants impose a final filter through their genetic makeup and expressed traits. However, testing this framework is challenging because spatial and environmental variation are often confounded, environmental conditions can alter host trait expression, and the specific plant traits mediating host genetic effects remain largely mysterious. While agricultural systems have provided valuable mechanistic insights, wild plant populations remain critically understudied, particularly through experimental approaches that can establish causation rather than correlation. I address these challenges through a novel ecological genetics approach that combines observational surveys with experimental manipulations of host origin and genotype in wild populations. Working with Clarkia xantiana, a wild annual wildflower native to California's Sierra Nevada foothills, I progressively narrow spatial scale while increasing experimental control. In Chapter I, I surveyed endophyte communities across 50 sites spanning 100 kilometers to quantify how community structure is associated with geographic distance, environmental factors, and host identity. I separated out these often-confounded factors by repeatedly co-sampling three herbaceous plant species across a topographically complex landscape where space and environment are largely decoupled. Our study reveals that community composition shows strong associations with spatial distance, while alpha diversity varies in relation to environmental gradients differently across host species. Within sites, host species harbor largely distinct communities, yet the specific taxa associated with each host vary inconsistently across locations. This chapter establishes that different assembly processes may affect different axes of community variation and that the strength of host associations is scale-dependent.In Chapter II, I implement a fully reciprocal transplant experiment across four sites, growing plants from four genetically differentiated source populations at all four sites in a factorial design. Despite clear fitness variation among populations, I find that site effects overwhelm source population influences on endophyte communities. Local environmental filtering and the available microbial species pool appear far more important than host population genetics, even though populations differ in traits potentially relevant to microbe interactions. This reveals that while host species identity matters, naturally occurring intraspecific genetic variation has minimal detectable effects under realistic field conditions.In Chapter III, I take an experimental ecological genetic approach, manipulating host genotype within a single site using recombinant inbred lines that segregate for leaf anthocyanin content. By creating genetic variation in this target trait that exceeds what is found in natural populations, I test mechanistic hypotheses about trait-mediated assembly. Growing 48 genetic lines under factorial water and fungicide treatments, I find that anthocyanin content strongly predicts both community diversity and composition, with high-anthocyanin plants supporting greater diversity while excluding potentially pathogenic taxa that dominate low-anthocyanin plants. This work demonstrates that host chemical traits can mechanistically filter fungal colonization, revealing that while naturally occurring population-level variation may be insufficient to structure communities (Chapter II), experimental manipulation of genetic variation exposes underlying trait-based mechanisms. This establishes that secondary metabolite variation represents a fundamental organizing principle in foliar microbiome assembly, with implications for understanding how plant chemical diversity shapes microbial communities and how these interactions might evolve or be manipulated. Taken together, this work establishes a hierarchical framework where regional dispersal patterns, local environmental filtering, and host chemical traits interact across scales to shape microbial community assembly in natural ecosystems. By revealing which processes operate at which scales and identifying specific mechanisms of host filtering through experimental manipulation in wild plant populations-an approach rarely applied to research on foliar endophytes-this dissertation provides a conceptual foundation for understanding the community assembly frameworks that shape plant microbiomes.
■590 ▼aSchool code: 0130.
■650 4▼aEcology
■650 4▼aMicrobiology
■650 4▼aBotany
■650 4▼aGenetics
■653 ▼aAnthocyanins
■653 ▼aCommunity assembly
■653 ▼aEnvironmental filters
■653 ▼aFungal endophytes
■653 ▼aGenetic filters
■653 ▼aPlant microbiome
■690 ▼a0329
■690 ▼a0410
■690 ▼a0309
■690 ▼a0369
■71020▼aUniversity of Minnesota▼bEcology, Evolution and Behavior.
■7730 ▼tDissertations Abstracts International▼g87-05B.
■790 ▼a0130
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17360040▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.


