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Untangling Spatial, Environmental, and Genetic Drivers of Foliar Endophyte Community Assembly
Untangling Spatial, Environmental, and Genetic Drivers of Foliar Endophyte Community Assem...
Untangling Spatial, Environmental, and Genetic Drivers of Foliar Endophyte Community Assembly

상세정보

자료유형  
 학위논문 서양
최종처리일시  
20260202105255
ISBN  
9798263319304
DDC  
574.5
저자명  
Mueller, Taz.
서명/저자  
Untangling Spatial, Environmental, and Genetic Drivers of Foliar Endophyte Community Assembly
발행사항  
[Sl] : University of Minnesota, 2025
발행사항  
Ann Arbor : ProQuest Dissertations & Theses, 2025
형태사항  
133 p
주기사항  
Source: Dissertations Abstracts International, Volume: 87-05, Section: B.
주기사항  
Advisor: Moeller, David A.
학위논문주기  
Thesis (Ph.D.)--University of Minnesota, 2025.
초록/해제  
요약Plants and their microbiomes form integrated systems where the boundaries between host and microbe are often blurred. Foliar fungal endophytes-fungi living asymptomatically inside healthy leaves-colonize virtually all terrestrial plants and can profoundly influence host performance through effects on stress tolerance, pathogen resistance, and resource acquisition. Despite their ubiquity and ecological importance, we lack fundamental understanding of what determines which fungi colonize which plants. The central challenge is that multiple processes simultaneously shape communities-dispersal moves microbes across landscapes, environmental conditions filter which taxa can persist, and host plants selectively permit or exclude colonizers based on their traits-but these processes operate at different spatial scales and interact in complex ways that are rarely studied together, particularly in natural field environments.Understanding microbial community assembly requires disentangling processes that operate hierarchically across biological scales. Regional dispersal patterns determine which taxa are present in a landscape, local environmental conditions filter this regional species pool, and host plants impose a final filter through their genetic makeup and expressed traits. However, testing this framework is challenging because spatial and environmental variation are often confounded, environmental conditions can alter host trait expression, and the specific plant traits mediating host genetic effects remain largely mysterious. While agricultural systems have provided valuable mechanistic insights, wild plant populations remain critically understudied, particularly through experimental approaches that can establish causation rather than correlation. I address these challenges through a novel ecological genetics approach that combines observational surveys with experimental manipulations of host origin and genotype in wild populations. Working with Clarkia xantiana, a wild annual wildflower native to California's Sierra Nevada foothills, I progressively narrow spatial scale while increasing experimental control. In Chapter I, I surveyed endophyte communities across 50 sites spanning 100 kilometers to quantify how community structure is associated with geographic distance, environmental factors, and host identity. I separated out these often-confounded factors by repeatedly co-sampling three herbaceous plant species across a topographically complex landscape where space and environment are largely decoupled. Our study reveals that community composition shows strong associations with spatial distance, while alpha diversity varies in relation to environmental gradients differently across host species. Within sites, host species harbor largely distinct communities, yet the specific taxa associated with each host vary inconsistently across locations. This chapter establishes that different assembly processes may affect different axes of community variation and that the strength of host associations is scale-dependent.In Chapter II, I implement a fully reciprocal transplant experiment across four sites, growing plants from four genetically differentiated source populations at all four sites in a factorial design. Despite clear fitness variation among populations, I find that site effects overwhelm source population influences on endophyte communities. Local environmental filtering and the available microbial species pool appear far more important than host population genetics, even though populations differ in traits potentially relevant to microbe interactions. This reveals that while host species identity matters, naturally occurring intraspecific genetic variation has minimal detectable effects under realistic field conditions.In Chapter III, I take an experimental ecological genetic approach, manipulating host genotype within a single site using recombinant inbred lines that segregate for leaf anthocyanin content. By creating genetic variation in this target trait that exceeds what is found in natural populations, I test mechanistic hypotheses about trait-mediated assembly. Growing 48 genetic lines under factorial water and fungicide treatments, I find that anthocyanin content strongly predicts both community diversity and composition, with high-anthocyanin plants supporting greater diversity while excluding potentially pathogenic taxa that dominate low-anthocyanin plants. This work demonstrates that host chemical traits can mechanistically filter fungal colonization, revealing that while naturally occurring population-level variation may be insufficient to structure communities (Chapter II), experimental manipulation of genetic variation exposes underlying trait-based mechanisms. This establishes that secondary metabolite variation represents a fundamental organizing principle in foliar microbiome assembly, with implications for understanding how plant chemical diversity shapes microbial communities and how these interactions might evolve or be manipulated. Taken together, this work establishes a hierarchical framework where regional dispersal patterns, local environmental filtering, and host chemical traits interact across scales to shape microbial community assembly in natural ecosystems. By revealing which processes operate at which scales and identifying specific mechanisms of host filtering through experimental manipulation in wild plant populations-an approach rarely applied to research on foliar endophytes-this dissertation provides a conceptual foundation for understanding the community assembly frameworks that shape plant microbiomes.
일반주제명  
Ecology
일반주제명  
Microbiology
일반주제명  
Botany
일반주제명  
Genetics
키워드  
Anthocyanins
키워드  
Community assembly
키워드  
Environmental filters
키워드  
Fungal endophytes
키워드  
Genetic filters
키워드  
Plant microbiome
기타저자  
University of Minnesota Ecology Evolution and Behavior
기본자료저록  
Dissertations Abstracts International. 87-05B.
전자적 위치 및 접속  
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MARC

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■1001  ▼aMueller,  Taz.
■24510▼aUntangling  Spatial,  Environmental,  and  Genetic  Drivers  of  Foliar  Endophyte  Community  Assembly
■260    ▼a[Sl]▼bUniversity  of  Minnesota▼c2025
■260  1▼aAnn  Arbor▼bProQuest  Dissertations  &  Theses▼c2025
■300    ▼a133  p
■500    ▼aSource:  Dissertations  Abstracts  International,  Volume:  87-05,  Section:  B.
■500    ▼aAdvisor:  Moeller,  David  A.
■5021  ▼aThesis  (Ph.D.)--University  of  Minnesota,  2025.
■520    ▼aPlants  and  their  microbiomes  form  integrated  systems  where  the  boundaries  between  host  and  microbe  are  often  blurred.  Foliar  fungal  endophytes-fungi  living  asymptomatically  inside  healthy  leaves-colonize  virtually  all  terrestrial  plants  and  can  profoundly  influence  host  performance  through  effects  on  stress  tolerance,  pathogen  resistance,  and  resource  acquisition.  Despite  their  ubiquity  and  ecological  importance,  we  lack  fundamental  understanding  of  what  determines  which  fungi  colonize  which  plants.  The  central  challenge  is  that  multiple  processes  simultaneously  shape  communities-dispersal  moves  microbes  across  landscapes,  environmental  conditions  filter  which  taxa  can  persist,  and  host  plants  selectively  permit  or  exclude  colonizers  based  on  their  traits-but  these  processes  operate  at  different  spatial  scales  and  interact  in  complex  ways  that  are  rarely  studied  together,  particularly  in  natural  field  environments.Understanding  microbial  community  assembly  requires  disentangling  processes  that  operate  hierarchically  across  biological  scales.  Regional  dispersal  patterns  determine  which  taxa  are  present  in  a  landscape,  local  environmental  conditions  filter  this  regional  species  pool,  and  host  plants  impose  a  final  filter  through  their  genetic  makeup  and  expressed  traits.  However,  testing  this  framework  is  challenging  because  spatial  and  environmental  variation  are  often  confounded,  environmental  conditions  can  alter  host  trait  expression,  and  the  specific  plant  traits  mediating  host  genetic  effects  remain  largely  mysterious.  While  agricultural  systems  have  provided  valuable  mechanistic  insights,  wild  plant  populations  remain  critically  understudied,  particularly  through  experimental  approaches  that  can  establish  causation  rather  than  correlation.  I  address  these  challenges  through  a  novel  ecological  genetics  approach  that  combines  observational  surveys  with  experimental  manipulations  of  host  origin  and  genotype  in  wild  populations.  Working  with  Clarkia  xantiana,  a  wild  annual  wildflower  native  to    California's  Sierra  Nevada  foothills,  I  progressively  narrow  spatial  scale  while  increasing  experimental  control.  In  Chapter  I,  I  surveyed  endophyte  communities  across  50  sites  spanning  100  kilometers  to  quantify  how  community  structure  is  associated  with  geographic  distance,  environmental  factors,  and  host  identity.  I  separated  out  these  often-confounded  factors  by  repeatedly  co-sampling  three  herbaceous  plant  species  across  a  topographically  complex  landscape  where  space  and  environment  are  largely  decoupled.  Our  study  reveals  that  community  composition  shows  strong  associations  with  spatial  distance,  while  alpha  diversity  varies  in  relation  to  environmental  gradients  differently  across  host  species.  Within  sites,  host  species  harbor  largely  distinct  communities,  yet  the  specific  taxa  associated  with  each  host  vary  inconsistently  across  locations.  This  chapter  establishes  that  different  assembly  processes  may  affect  different  axes  of  community  variation  and  that  the  strength  of  host  associations  is  scale-dependent.In  Chapter  II,  I  implement  a  fully  reciprocal  transplant  experiment  across  four  sites,  growing  plants  from  four  genetically  differentiated  source  populations  at  all  four  sites  in  a  factorial  design.  Despite  clear  fitness  variation  among  populations,  I  find  that  site  effects  overwhelm  source  population  influences  on  endophyte  communities.  Local  environmental  filtering  and  the  available  microbial  species  pool  appear  far  more  important  than  host  population  genetics,  even  though  populations  differ  in  traits  potentially  relevant  to  microbe  interactions.  This  reveals  that  while  host  species  identity  matters,  naturally  occurring  intraspecific  genetic  variation  has  minimal  detectable  effects  under  realistic  field  conditions.In  Chapter  III,  I  take  an  experimental  ecological  genetic  approach,  manipulating  host  genotype  within  a  single  site  using  recombinant  inbred  lines  that  segregate  for  leaf  anthocyanin  content.  By  creating  genetic  variation  in  this  target  trait  that  exceeds  what  is  found  in  natural  populations,  I  test  mechanistic  hypotheses  about  trait-mediated  assembly.  Growing  48  genetic  lines  under  factorial  water  and  fungicide  treatments,  I  find  that  anthocyanin  content  strongly  predicts  both  community  diversity  and  composition,  with  high-anthocyanin  plants  supporting  greater  diversity  while  excluding  potentially  pathogenic  taxa  that  dominate  low-anthocyanin  plants.  This  work  demonstrates  that  host  chemical  traits  can  mechanistically  filter  fungal  colonization,  revealing  that  while  naturally  occurring  population-level  variation  may  be  insufficient  to  structure  communities  (Chapter  II),  experimental  manipulation  of  genetic  variation  exposes  underlying  trait-based  mechanisms.  This  establishes  that  secondary  metabolite  variation  represents  a  fundamental  organizing  principle  in  foliar  microbiome  assembly,  with  implications  for  understanding  how  plant  chemical  diversity  shapes  microbial  communities  and  how  these  interactions  might  evolve  or  be  manipulated.  Taken  together,  this  work  establishes  a  hierarchical  framework  where  regional  dispersal  patterns,  local  environmental  filtering,  and  host  chemical  traits  interact  across  scales  to  shape  microbial  community  assembly  in  natural  ecosystems.  By  revealing  which  processes  operate  at  which  scales  and  identifying  specific  mechanisms  of  host  filtering  through  experimental  manipulation  in  wild  plant  populations-an  approach  rarely  applied  to  research  on  foliar  endophytes-this  dissertation  provides  a  conceptual  foundation  for  understanding  the  community  assembly  frameworks  that  shape  plant  microbiomes.
■590    ▼aSchool  code:  0130.
■650  4▼aEcology
■650  4▼aMicrobiology
■650  4▼aBotany
■650  4▼aGenetics
■653    ▼aAnthocyanins
■653    ▼aCommunity  assembly
■653    ▼aEnvironmental  filters
■653    ▼aFungal  endophytes
■653    ▼aGenetic  filters
■653    ▼aPlant  microbiome
■690    ▼a0329
■690    ▼a0410
■690    ▼a0309
■690    ▼a0369
■71020▼aUniversity  of  Minnesota▼bEcology,  Evolution  and  Behavior.
■7730  ▼tDissertations  Abstracts  International▼g87-05B.
■790    ▼a0130
■791    ▼aPh.D.
■792    ▼a2025
■793    ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17360040▼nKERIS▼z이  자료의  원문은  한국교육학술정보원에서  제공합니다.

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