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From Single Cells to Organs: Cell Type Specific Transcription and Signaling for Robust Patterning and Growth of the Arabidopsis thaliana Sepal
From Single Cells to Organs: Cell Type Specific Transcription and Signaling for Robust Patterning and Growth of the Arabidopsis thaliana Sepal
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202104725
- ISBN
- 9798293823055
- DDC
- 580
- 저자명
- Rusnak, Byron.
- 서명/저자
- From Single Cells to Organs: Cell Type Specific Transcription and Signaling for Robust Patterning and Growth of the Arabidopsis thaliana Sepal
- 발행사항
- [Sl] : Cornell University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 188 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-03, Section: B.
- 주기사항
- Advisor: Roeder, Adrienne.
- 학위논문주기
- Thesis (Ph.D.)--Cornell University, 2025.
- 초록/해제
- 요약Organ formation is a highly dynamic process that involves the coordinated initiation of organogenesis, outgrowth, and differentiation of many cell types. This requires highly regulated gene expression within individual cells, and across entire organs, and must remain robust to external perturbations. In this dissertation, I use the Arabidopsis thaliana sepal as a model to investigate robustness in gene expression and organ growth.In chapter 1, I explore the question of "what is a cell type?" The concept of a cell type has evolved with modern research methods like single cell RNA-sequencing and findings that differentiated cell de-differentiate and re-differentiate under the certain mechanical and/or transcriptional cues. Biological research continues to broaden our appreciation for the richness in diversity of cell types, intermediates, developmental trajectories, and plasticity across multicellular organisms.In chapter 2, I delve deeper into the field of cell type biology by dissecting a 1 kilobase enhancer sequence that confers cell type specific expression to giant cells. This occurs through the combinatorial action of three transcription factor classes - DOFs, class IV HD-ZIPs, and class II TCPs - none of which individually has giant cell specific expression. Rather, TCPs activate expression throughout the entire epidermis, HD-ZIPs enhance expression levels in the giant cells, and DOFs decrease expression enough to suppress expression in the small cells, but not in giant cells.In chapter 3, I use single cell RNA-seq and spatial transcriptomics to identify key cell types in young flower buds in wild-type and in the mutant drmy1, which has lost robust sepal initiation and outgrowth. I detect upregulation in brassinosteroid (BR) signaling in drmy1, particularly in meristematic and epidermal cell types. Using chemical treatments and BR mutants, I demonstrate that increasing or decreasing brassinosteroid signaling has differential effects on different sepals of the same flower bud.My work reveals the importance of cell type specificity in gene expression and signaling for development. Cells must integrate multiple transcription factors in a combinatorial fashion to generate cell type specific gene expression patterns, and in turn, organ development requires specific levels and patterns of various transcriptional networks to achieve proper growth patterns.
- 일반주제명
- Plant sciences
- 일반주제명
- Developmental biology
- 일반주제명
- Genetics
- 키워드
- Brassinosteroid
- 키워드
- Cell type
- 키워드
- Gene expression
- 기타저자
- Cornell University Plant Biology
- 기본자료저록
- Dissertations Abstracts International. 87-03B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■006m o d
■007cr#unu||||||||
■020 ▼a9798293823055
■035 ▼a(MiAaPQ)AAI32122065
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a580
■1001 ▼aRusnak, Byron.▼0(orcid)0000000174139468
■24510▼aFrom Single Cells to Organs: Cell Type Specific Transcription and Signaling for Robust Patterning and Growth of the Arabidopsis thaliana Sepal
■260 ▼a[Sl]▼bCornell University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a188 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-03, Section: B.
■500 ▼aAdvisor: Roeder, Adrienne.
■5021 ▼aThesis (Ph.D.)--Cornell University, 2025.
■520 ▼aOrgan formation is a highly dynamic process that involves the coordinated initiation of organogenesis, outgrowth, and differentiation of many cell types. This requires highly regulated gene expression within individual cells, and across entire organs, and must remain robust to external perturbations. In this dissertation, I use the Arabidopsis thaliana sepal as a model to investigate robustness in gene expression and organ growth.In chapter 1, I explore the question of "what is a cell type?" The concept of a cell type has evolved with modern research methods like single cell RNA-sequencing and findings that differentiated cell de-differentiate and re-differentiate under the certain mechanical and/or transcriptional cues. Biological research continues to broaden our appreciation for the richness in diversity of cell types, intermediates, developmental trajectories, and plasticity across multicellular organisms.In chapter 2, I delve deeper into the field of cell type biology by dissecting a 1 kilobase enhancer sequence that confers cell type specific expression to giant cells. This occurs through the combinatorial action of three transcription factor classes - DOFs, class IV HD-ZIPs, and class II TCPs - none of which individually has giant cell specific expression. Rather, TCPs activate expression throughout the entire epidermis, HD-ZIPs enhance expression levels in the giant cells, and DOFs decrease expression enough to suppress expression in the small cells, but not in giant cells.In chapter 3, I use single cell RNA-seq and spatial transcriptomics to identify key cell types in young flower buds in wild-type and in the mutant drmy1, which has lost robust sepal initiation and outgrowth. I detect upregulation in brassinosteroid (BR) signaling in drmy1, particularly in meristematic and epidermal cell types. Using chemical treatments and BR mutants, I demonstrate that increasing or decreasing brassinosteroid signaling has differential effects on different sepals of the same flower bud.My work reveals the importance of cell type specificity in gene expression and signaling for development. Cells must integrate multiple transcription factors in a combinatorial fashion to generate cell type specific gene expression patterns, and in turn, organ development requires specific levels and patterns of various transcriptional networks to achieve proper growth patterns.
■590 ▼aSchool code: 0058.
■650 4▼aPlant sciences
■650 4▼aDevelopmental biology
■650 4▼aGenetics
■653 ▼aArabidopsis thaliana
■653 ▼aBrassinosteroid
■653 ▼aCell type
■653 ▼aOrgan development
■653 ▼aGene expression
■690 ▼a0479
■690 ▼a0758
■690 ▼a0369
■71020▼aCornell University▼bPlant Biology.
■7730 ▼tDissertations Abstracts International▼g87-03B.
■790 ▼a0058
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17358603▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
