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Investigating the Function and Evolutionary Context of WI12Rhg1 in Soybean Cyst Nematode Resistance
Investigating the Function and Evolutionary Context of WI12Rhg1 in Soybean Cyst Nematode Resistance
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105124
- ISBN
- 9798291547465
- DDC
- 581
- 서명/저자
- Investigating the Function and Evolutionary Context of WI12Rhg1 in Soybean Cyst Nematode Resistance
- 발행사항
- [Sl] : The University of Wisconsin - Madison, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 166 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-02, Section: B.
- 주기사항
- Advisor: Bent, Andrew.
- 학위논문주기
- Thesis (Ph.D.)--The University of Wisconsin - Madison, 2025.
- 초록/해제
- 요약Soybean cyst nematode (SCN, Heterodera glycines) is the most economically damaging pathogen of soybean. Resistance to SCN in commercial soybean cultivars primarily depends on the Rhg1 locus, a multi-gene locus that encodes three proteins that contribute to SCN resistance. The least-characterized Rhg1-encoded protein is the wound-inducible domain-containing protein WI12Rhg1 encoded by Glyma.18G022700. WI12 proteins have no known function but have been implicated in reproductive development and responses to various biotic and abiotic stresses. This thesis research investigated the function and evolutionary context of WI12Rhg1 in SCN resistance. Silencing WI12Rhg1 in whole plants compromised resistance to both HG 0 and HG 2.5.7 SCN populations, demonstrating that WI12Rhg1 expression is necessary for full rhg1-b-mediated resistance. Transcriptomic and metabolomic experiments revealed that silencing WI12Rhg1 resulted in widespread transcriptional changes, dramatically reduced accumulation of salicylic acid, shifted the flux of phenylpropanoid metabolism away from downstream products, inhibited induction of defense signaling pathways, and caused dysregulation of gibberellic acid (GA) signaling. This work also clarified conflicting reports regarding the impact of GA on SCN resistance, as exogenous application of GA increased SCN resistance. RNA-seq data and RT-qPCR results revealed that WI12Rhg1 may impact DELLA stability as well as mir159 expression, which have been implicated in GA-mediated SCN resistance. Exploration of WI12Rhg1 gene structure revealed the presence of a non-canonical CUG translation start site upstream of the annotated ATG start site and found that this WI12 CUG and resulting N-terminal extension are highly conserved across land plant lineages. Analyses of WI12 gene structure, phylogeny, and M. truncatula Ribo-seq data provided multiple lines of evidence for translation initiation occurring at the CUG. Immunoblotting revealed that the observed WI12Rhg1 protein changes in different expression systems and that WI12Rhg1 forms high molecular weight complexes, potentially due to stable protein-protein interactions. Immunoblotting of myc-tagged WI12Rhg1 confirmed that translation from the CUG position can yield a stable protein in soybean and Nicotiana benthamiana, however endogenous use of the CUG start site remains unconfirmed. Together, these findings establish WI12Rhg1 as a broad modulator of plant defense, hormone signaling and metabolic balance, and suggest non-canonical translation initiation in the WI12 gene family.
- 일반주제명
- Plant pathology
- 일반주제명
- Molecular biology
- 일반주제명
- Plant sciences
- 일반주제명
- Immunology
- 키워드
- WI12 proteins
- 키워드
- Immunoblotting
- 기타저자
- The University of Wisconsin - Madison Plant Pathology
- 기본자료저록
- Dissertations Abstracts International. 87-02B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798291547465
■035 ▼a(MiAaPQ)AAI32238613
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a581
■1001 ▼aLowenstein, Aaron.
■24510▼aInvestigating the Function and Evolutionary Context of WI12Rhg1 in Soybean Cyst Nematode Resistance
■260 ▼a[Sl]▼bThe University of Wisconsin - Madison▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a166 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-02, Section: B.
■500 ▼aAdvisor: Bent, Andrew.
■5021 ▼aThesis (Ph.D.)--The University of Wisconsin - Madison, 2025.
■520 ▼aSoybean cyst nematode (SCN, Heterodera glycines) is the most economically damaging pathogen of soybean. Resistance to SCN in commercial soybean cultivars primarily depends on the Rhg1 locus, a multi-gene locus that encodes three proteins that contribute to SCN resistance. The least-characterized Rhg1-encoded protein is the wound-inducible domain-containing protein WI12Rhg1 encoded by Glyma.18G022700. WI12 proteins have no known function but have been implicated in reproductive development and responses to various biotic and abiotic stresses. This thesis research investigated the function and evolutionary context of WI12Rhg1 in SCN resistance. Silencing WI12Rhg1 in whole plants compromised resistance to both HG 0 and HG 2.5.7 SCN populations, demonstrating that WI12Rhg1 expression is necessary for full rhg1-b-mediated resistance. Transcriptomic and metabolomic experiments revealed that silencing WI12Rhg1 resulted in widespread transcriptional changes, dramatically reduced accumulation of salicylic acid, shifted the flux of phenylpropanoid metabolism away from downstream products, inhibited induction of defense signaling pathways, and caused dysregulation of gibberellic acid (GA) signaling. This work also clarified conflicting reports regarding the impact of GA on SCN resistance, as exogenous application of GA increased SCN resistance. RNA-seq data and RT-qPCR results revealed that WI12Rhg1 may impact DELLA stability as well as mir159 expression, which have been implicated in GA-mediated SCN resistance. Exploration of WI12Rhg1 gene structure revealed the presence of a non-canonical CUG translation start site upstream of the annotated ATG start site and found that this WI12 CUG and resulting N-terminal extension are highly conserved across land plant lineages. Analyses of WI12 gene structure, phylogeny, and M. truncatula Ribo-seq data provided multiple lines of evidence for translation initiation occurring at the CUG. Immunoblotting revealed that the observed WI12Rhg1 protein changes in different expression systems and that WI12Rhg1 forms high molecular weight complexes, potentially due to stable protein-protein interactions. Immunoblotting of myc-tagged WI12Rhg1 confirmed that translation from the CUG position can yield a stable protein in soybean and Nicotiana benthamiana, however endogenous use of the CUG start site remains unconfirmed. Together, these findings establish WI12Rhg1 as a broad modulator of plant defense, hormone signaling and metabolic balance, and suggest non-canonical translation initiation in the WI12 gene family.
■590 ▼aSchool code: 0262.
■650 4▼aPlant pathology
■650 4▼aMolecular biology
■650 4▼aPlant sciences
■650 4▼aImmunology
■653 ▼aSoybean cyst nematode
■653 ▼aWI12 proteins
■653 ▼aGibberellic acid signaling
■653 ▼aImmunoblotting
■653 ▼aNicotiana benthamiana
■690 ▼a0480
■690 ▼a0307
■690 ▼a0479
■690 ▼a0982
■71020▼aThe University of Wisconsin - Madison▼bPlant Pathology.
■7730 ▼tDissertations Abstracts International▼g87-02B.
■790 ▼a0262
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17359474▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
