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Cellular and Molecular Mechanisms of Blood-Brain and Blood-Spinal Cord Barrier Heterogeneity During Development
Cellular and Molecular Mechanisms of Blood-Brain and Blood-Spinal Cord Barrier Heterogeneity During Development
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105304
- ISBN
- 9798270226480
- DDC
- 574
- 서명/저자
- Cellular and Molecular Mechanisms of Blood-Brain and Blood-Spinal Cord Barrier Heterogeneity During Development
- 발행사항
- [Sl] : University of California, San Francisco, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 163 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-06, Section: B.
- 주기사항
- Advisor: MacKenzie, Tippi C.;Paredes, Mercedes F.
- 학위논문주기
- Thesis (Ph.D.)--University of California, San Francisco, 2025.
- 초록/해제
- 요약The blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) regulate exchange between the peripheral circulation and the central nervous system (CNS). During development, these barriers have a selective permeability that differs from adult states, creating both vulnerabilities and therapeutic opportunities. This dissertation addresses fundamental questions about blood-CNS barrier during development. (1) What endothelial and mural cell subtypes are present during early CNS vascular development? (2) How do these subtypes map to regions of differing barrier permeability? (3) What molecular signatures distinguish these regions? Chapter 2 presents a single-cell atlas of mouse brain and spinal cord vasculature at embryonic day (E)13.5 and E18.5, identifying endothelial and mural subtypes that establish arteriovenous zonation. Trajectory analysis revealed maturation programs progressing from proliferation through angiogenesis to transporter-rich barrier states, with region-specific signatures distinguishing brain from spinal cord. Chapter 3 developed a "BBB scorecard" quantifying CNS-specific signatures. Combined with permeability mapping and RNA in situ hybridization, permeable zones align with specific vascular subtypes. Col15a1 was identified as a potential marker of permeable territories. Chapter 4 investigated immune-vascular interactions, identifying galectin-9 as a potential mediator and CD206+ macrophages in multiple positions relative to vessels, consistent with transmigration. These findings establish underscore vascular subtype composition and regional specialization in blood-CNS barrier function.
- 일반주제명
- Developmental biology
- 일반주제명
- Cellular biology
- 일반주제명
- Neurosciences
- 키워드
- Neurovasculature
- 키워드
- Mouse brain
- 기타저자
- University of California, San Francisco Biomedical Sciences
- 기본자료저록
- Dissertations Abstracts International. 87-06B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520260202105304
■006m o d
■007cr#unu||||||||
■020 ▼a9798270226480
■035 ▼a(MiAaPQ)AAI32282827
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aWedderburn-Pugh, Kaylee.
■24510▼aCellular and Molecular Mechanisms of Blood-Brain and Blood-Spinal Cord Barrier Heterogeneity During Development
■260 ▼a[Sl]▼bUniversity of California, San Francisco▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a163 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-06, Section: B.
■500 ▼aAdvisor: MacKenzie, Tippi C.;Paredes, Mercedes F.
■5021 ▼aThesis (Ph.D.)--University of California, San Francisco, 2025.
■520 ▼aThe blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) regulate exchange between the peripheral circulation and the central nervous system (CNS). During development, these barriers have a selective permeability that differs from adult states, creating both vulnerabilities and therapeutic opportunities. This dissertation addresses fundamental questions about blood-CNS barrier during development. (1) What endothelial and mural cell subtypes are present during early CNS vascular development? (2) How do these subtypes map to regions of differing barrier permeability? (3) What molecular signatures distinguish these regions? Chapter 2 presents a single-cell atlas of mouse brain and spinal cord vasculature at embryonic day (E)13.5 and E18.5, identifying endothelial and mural subtypes that establish arteriovenous zonation. Trajectory analysis revealed maturation programs progressing from proliferation through angiogenesis to transporter-rich barrier states, with region-specific signatures distinguishing brain from spinal cord. Chapter 3 developed a "BBB scorecard" quantifying CNS-specific signatures. Combined with permeability mapping and RNA in situ hybridization, permeable zones align with specific vascular subtypes. Col15a1 was identified as a potential marker of permeable territories. Chapter 4 investigated immune-vascular interactions, identifying galectin-9 as a potential mediator and CD206+ macrophages in multiple positions relative to vessels, consistent with transmigration. These findings establish underscore vascular subtype composition and regional specialization in blood-CNS barrier function.
■590 ▼aSchool code: 0034.
■650 4▼aDevelopmental biology
■650 4▼aCellular biology
■650 4▼aNeurosciences
■653 ▼aBlood-brain barrier
■653 ▼aBlood-spinal cord barrier
■653 ▼aNeurovasculature
■653 ▼aCentral nervous system
■653 ▼aMouse brain
■690 ▼a0758
■690 ▼a0379
■690 ▼a0317
■71020▼aUniversity of California, San Francisco▼bBiomedical Sciences.
■7730 ▼tDissertations Abstracts International▼g87-06B.
■790 ▼a0034
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17360103▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
