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Characterization of Postharvest Diseases, and Their Interaction With In-Season Cercospora Leaf Spot, Impacting Sugarbeet Storage in Michigan
Characterization of Postharvest Diseases, and Their Interaction With In-Season Cercospora Leaf Spot, Impacting Sugarbeet Storage in Michigan
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105305
- ISBN
- 9798263322236
- DDC
- 581
- 서명/저자
- Characterization of Postharvest Diseases, and Their Interaction With In-Season Cercospora Leaf Spot, Impacting Sugarbeet Storage in Michigan
- 발행사항
- [Sl] : Michigan State University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 165 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-05, Section: B.
- 주기사항
- Advisor: Willbur, Jaime.
- 학위논문주기
- Thesis (Ph.D.)--Michigan State University, 2025.
- 초록/해제
- 요약Sugarbeet (Beta vulgaris) is a major agricultural crop in the United States, responsible for over half of domestic sugar production. Following harvest, sugarbeet roots grown in many regions are stored in large outdoor piles for up to 200 days before processing, making them vulnerable to postharvest degradation. Storage losses primarily occur through respiration and microbial rot, both of which reduce sucrose content and quality. Cercospora leaf spot (CLS) has been considered a potential predisposing factor for increased storage rot. In the current study, across three CLS-susceptible commercial varieties, there were no significant differences between storage rot susceptibility to any of the three tested pathogens in hand-harvested sugarbeets, regardless of CLS level, at any storage timepoint in 2020 or 2021 (P 0.05). In studies using CLS-susceptible and -resistant germplasm and varieties, CLS effects were inconsistent and only significant in one parameter at two of six storage timepoints across years (P 0.05). With the tested storage pathogens, prior CLS level also did not impact root respiration or the change in respiration rate from initial to final storage timepoint in either 2021 or 2023 (P 0.05). These results showed that foliar CLS infection alone may not predispose beets to storage rot. Mechanical harvest, on the other hand, substantially increased disease severity across all treatments, confirming that physical damage is a more critical factor in postharvest rot than in-season foliar stress.Commercial storage facilities continue to stress anecdotal evidence of CLS impacting storability of sugar beets. To further investigate the impact of CLS on commercial storage, post-harvest symptom development was evaluated in bruised beets with relatively high or low in-season CLS severity in 2022 and 2023. Bruised and non-bruised tissue of roots of each CLS level were inoculated with known storage pathogens, and while bruising did significantly impact rot development at all timepoints (P 0.05), CLS did not impact rate of rot development in bruised beets.To inform future breeding efforts, twelve commercial sugarbeet varieties were evaluated for their susceptibility to common storage rot pathogens across two storage seasons; Botrytis cinerea, Fusarium graminearum, and Penicillium vulpinum across two storage seasons. Roots were harvested in 2022 and 2023, sliced, and inoculated under controlled storage conditions. All varieties exhibited susceptibility to the pathogens, with B. cinerea consistently inducing the most severe rot. No variety demonstrated consistent resistance across both years and all pathogens. Interestingly, several varieties that showed minimal rot under hand-harvest conditions in 2022 developed significantly more severe symptoms following machine harvest in 2023, suggesting that mechanical damage may have a greater influence on rot development than varietal resistance alone. These findings highlight the need for broader screening efforts that incorporate diverse pathogen populations, harvest methods, and more representative storage conditions. Breeding for postharvest resistance remains a largely untapped avenue with strong potential to reduce losses and enhance the long-term storability of sugarbeet crops.A multi-year pathogen survey to identify the fungal organisms most commonly associated with storage rot in Michigan was conducted from 2020-2022 and in 2024. A focus on Penicillium spp. and Fusarium spp. collected in 2024 were further identified using DNA sequencing. Molecular analysis identified five primary Fusarium species: F. oxysporum, F. fujikuroi, F. solani, F. graminearum, and F. acuminatum, as well as one isolate of F. tricinctum. Penicillium isolates included P. expansum, P. polonicum, P. crustosum, P. paneum, and P. brasilianum. These findings emphasize that sugarbeet storage rot in Michigan is caused by a diverse, changing fungal community and that pathogen levels and type may vary by year and site.Together, this research highlights the importance of both identifying storage pathogens and understanding how field and postharvest factors interact to affect disease severity. This research contributes to ongoing efforts to reduce postharvest losses, enhance sugar yield, and support more sustainable sugarbeet production systems.
- 일반주제명
- Plant pathology
- 일반주제명
- Microbiology
- 일반주제명
- Plant sciences
- 일반주제명
- Pathology
- 키워드
- Pathogens
- 키워드
- Storage rot
- 키워드
- Sugarbeet
- 키워드
- Michigan
- 기타저자
- Michigan State University Plant Pathology - Doctor of Philosophy
- 기본자료저록
- Dissertations Abstracts International. 87-05B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798263322236
■035 ▼a(MiAaPQ)AAI32283179
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a581
■1001 ▼aHendershot, Carly Nicole.
■24510▼aCharacterization of Postharvest Diseases, and Their Interaction With In-Season Cercospora Leaf Spot, Impacting Sugarbeet Storage in Michigan
■260 ▼a[Sl]▼bMichigan State University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a165 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-05, Section: B.
■500 ▼aAdvisor: Willbur, Jaime.
■5021 ▼aThesis (Ph.D.)--Michigan State University, 2025.
■520 ▼aSugarbeet (Beta vulgaris) is a major agricultural crop in the United States, responsible for over half of domestic sugar production. Following harvest, sugarbeet roots grown in many regions are stored in large outdoor piles for up to 200 days before processing, making them vulnerable to postharvest degradation. Storage losses primarily occur through respiration and microbial rot, both of which reduce sucrose content and quality. Cercospora leaf spot (CLS) has been considered a potential predisposing factor for increased storage rot. In the current study, across three CLS-susceptible commercial varieties, there were no significant differences between storage rot susceptibility to any of the three tested pathogens in hand-harvested sugarbeets, regardless of CLS level, at any storage timepoint in 2020 or 2021 (P 0.05). In studies using CLS-susceptible and -resistant germplasm and varieties, CLS effects were inconsistent and only significant in one parameter at two of six storage timepoints across years (P 0.05). With the tested storage pathogens, prior CLS level also did not impact root respiration or the change in respiration rate from initial to final storage timepoint in either 2021 or 2023 (P 0.05). These results showed that foliar CLS infection alone may not predispose beets to storage rot. Mechanical harvest, on the other hand, substantially increased disease severity across all treatments, confirming that physical damage is a more critical factor in postharvest rot than in-season foliar stress.Commercial storage facilities continue to stress anecdotal evidence of CLS impacting storability of sugar beets. To further investigate the impact of CLS on commercial storage, post-harvest symptom development was evaluated in bruised beets with relatively high or low in-season CLS severity in 2022 and 2023. Bruised and non-bruised tissue of roots of each CLS level were inoculated with known storage pathogens, and while bruising did significantly impact rot development at all timepoints (P 0.05), CLS did not impact rate of rot development in bruised beets.To inform future breeding efforts, twelve commercial sugarbeet varieties were evaluated for their susceptibility to common storage rot pathogens across two storage seasons; Botrytis cinerea, Fusarium graminearum, and Penicillium vulpinum across two storage seasons. Roots were harvested in 2022 and 2023, sliced, and inoculated under controlled storage conditions. All varieties exhibited susceptibility to the pathogens, with B. cinerea consistently inducing the most severe rot. No variety demonstrated consistent resistance across both years and all pathogens. Interestingly, several varieties that showed minimal rot under hand-harvest conditions in 2022 developed significantly more severe symptoms following machine harvest in 2023, suggesting that mechanical damage may have a greater influence on rot development than varietal resistance alone. These findings highlight the need for broader screening efforts that incorporate diverse pathogen populations, harvest methods, and more representative storage conditions. Breeding for postharvest resistance remains a largely untapped avenue with strong potential to reduce losses and enhance the long-term storability of sugarbeet crops.A multi-year pathogen survey to identify the fungal organisms most commonly associated with storage rot in Michigan was conducted from 2020-2022 and in 2024. A focus on Penicillium spp. and Fusarium spp. collected in 2024 were further identified using DNA sequencing. Molecular analysis identified five primary Fusarium species: F. oxysporum, F. fujikuroi, F. solani, F. graminearum, and F. acuminatum, as well as one isolate of F. tricinctum. Penicillium isolates included P. expansum, P. polonicum, P. crustosum, P. paneum, and P. brasilianum. These findings emphasize that sugarbeet storage rot in Michigan is caused by a diverse, changing fungal community and that pathogen levels and type may vary by year and site.Together, this research highlights the importance of both identifying storage pathogens and understanding how field and postharvest factors interact to affect disease severity. This research contributes to ongoing efforts to reduce postharvest losses, enhance sugar yield, and support more sustainable sugarbeet production systems.
■590 ▼aSchool code: 0128.
■650 4▼aPlant pathology
■650 4▼aMicrobiology
■650 4▼aPlant sciences
■650 4▼aPathology
■653 ▼aCercospora leaf spot
■653 ▼aPathogens
■653 ▼aStorage rot
■653 ▼aSugarbeet
■653 ▼aMichigan
■690 ▼a0480
■690 ▼a0410
■690 ▼a0479
■690 ▼a0571
■71020▼aMichigan State University▼bPlant Pathology - Doctor of Philosophy.
■7730 ▼tDissertations Abstracts International▼g87-05B.
■790 ▼a0128
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17360112▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.


