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Characterization of Postharvest Diseases, and Their Interaction With In-Season Cercospora Leaf Spot, Impacting Sugarbeet Storage in Michigan
Characterization of Postharvest Diseases, and Their Interaction With In-Season Cercospora ...
Characterization of Postharvest Diseases, and Their Interaction With In-Season Cercospora Leaf Spot, Impacting Sugarbeet Storage in Michigan

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자료유형  
 학위논문 서양
최종처리일시  
20260202105305
ISBN  
9798263322236
DDC  
581
저자명  
Hendershot, Carly Nicole.
서명/저자  
Characterization of Postharvest Diseases, and Their Interaction With In-Season Cercospora Leaf Spot, Impacting Sugarbeet Storage in Michigan
발행사항  
[Sl] : Michigan State University, 2025
발행사항  
Ann Arbor : ProQuest Dissertations & Theses, 2025
형태사항  
165 p
주기사항  
Source: Dissertations Abstracts International, Volume: 87-05, Section: B.
주기사항  
Advisor: Willbur, Jaime.
학위논문주기  
Thesis (Ph.D.)--Michigan State University, 2025.
초록/해제  
요약Sugarbeet (Beta vulgaris) is a major agricultural crop in the United States, responsible for over half of domestic sugar production. Following harvest, sugarbeet roots grown in many regions are stored in large outdoor piles for up to 200 days before processing, making them vulnerable to postharvest degradation. Storage losses primarily occur through respiration and microbial rot, both of which reduce sucrose content and quality. Cercospora leaf spot (CLS) has been considered a potential predisposing factor for increased storage rot. In the current study, across three CLS-susceptible commercial varieties, there were no significant differences between storage rot susceptibility to any of the three tested pathogens in hand-harvested sugarbeets, regardless of CLS level, at any storage timepoint in 2020 or 2021 (P 0.05). In studies using CLS-susceptible and -resistant germplasm and varieties, CLS effects were inconsistent and only significant in one parameter at two of six storage timepoints across years (P 0.05). With the tested storage pathogens, prior CLS level also did not impact root respiration or the change in respiration rate from initial to final storage timepoint in either 2021 or 2023 (P 0.05). These results showed that foliar CLS infection alone may not predispose beets to storage rot. Mechanical harvest, on the other hand, substantially increased disease severity across all treatments, confirming that physical damage is a more critical factor in postharvest rot than in-season foliar stress.Commercial storage facilities continue to stress anecdotal evidence of CLS impacting storability of sugar beets. To further investigate the impact of CLS on commercial storage, post-harvest symptom development was evaluated in bruised beets with relatively high or low in-season CLS severity in 2022 and 2023. Bruised and non-bruised tissue of roots of each CLS level were inoculated with known storage pathogens, and while bruising did significantly impact rot development at all timepoints (P 0.05), CLS did not impact rate of rot development in bruised beets.To inform future breeding efforts, twelve commercial sugarbeet varieties were evaluated for their susceptibility to common storage rot pathogens across two storage seasons; Botrytis cinerea, Fusarium graminearum, and Penicillium vulpinum across two storage seasons. Roots were harvested in 2022 and 2023, sliced, and inoculated under controlled storage conditions. All varieties exhibited susceptibility to the pathogens, with B. cinerea consistently inducing the most severe rot. No variety demonstrated consistent resistance across both years and all pathogens. Interestingly, several varieties that showed minimal rot under hand-harvest conditions in 2022 developed significantly more severe symptoms following machine harvest in 2023, suggesting that mechanical damage may have a greater influence on rot development than varietal resistance alone. These findings highlight the need for broader screening efforts that incorporate diverse pathogen populations, harvest methods, and more representative storage conditions. Breeding for postharvest resistance remains a largely untapped avenue with strong potential to reduce losses and enhance the long-term storability of sugarbeet crops.A multi-year pathogen survey to identify the fungal organisms most commonly associated with storage rot in Michigan was conducted from 2020-2022 and in 2024. A focus on Penicillium spp. and Fusarium spp. collected in 2024 were further identified using DNA sequencing. Molecular analysis identified five primary Fusarium species: F. oxysporum, F. fujikuroi, F. solani, F. graminearum, and F. acuminatum, as well as one isolate of F. tricinctum. Penicillium isolates included P. expansum, P. polonicum, P. crustosum, P. paneum, and P. brasilianum. These findings emphasize that sugarbeet storage rot in Michigan is caused by a diverse, changing fungal community and that pathogen levels and type may vary by year and site.Together, this research highlights the importance of both identifying storage pathogens and understanding how field and postharvest factors interact to affect disease severity. This research contributes to ongoing efforts to reduce postharvest losses, enhance sugar yield, and support more sustainable sugarbeet production systems.
일반주제명  
Plant pathology
일반주제명  
Microbiology
일반주제명  
Plant sciences
일반주제명  
Pathology
키워드  
Cercospora leaf spot
키워드  
Pathogens
키워드  
Storage rot
키워드  
Sugarbeet
키워드  
Michigan
기타저자  
Michigan State University Plant Pathology - Doctor of Philosophy
기본자료저록  
Dissertations Abstracts International. 87-05B.
전자적 위치 및 접속  
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MARC

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■1001  ▼aHendershot,  Carly  Nicole.
■24510▼aCharacterization  of  Postharvest  Diseases,  and  Their  Interaction  With  In-Season  Cercospora  Leaf  Spot,  Impacting  Sugarbeet  Storage  in  Michigan
■260    ▼a[Sl]▼bMichigan  State  University▼c2025
■260  1▼aAnn  Arbor▼bProQuest  Dissertations  &  Theses▼c2025
■300    ▼a165  p
■500    ▼aSource:  Dissertations  Abstracts  International,  Volume:  87-05,  Section:  B.
■500    ▼aAdvisor:  Willbur,  Jaime.
■5021  ▼aThesis  (Ph.D.)--Michigan  State  University,  2025.
■520    ▼aSugarbeet  (Beta  vulgaris)  is  a  major  agricultural  crop  in  the  United  States,  responsible  for  over  half  of  domestic  sugar  production.  Following  harvest,  sugarbeet  roots  grown  in  many  regions  are  stored  in  large  outdoor  piles  for  up  to  200  days  before  processing,  making  them  vulnerable  to  postharvest  degradation.  Storage  losses  primarily  occur  through  respiration  and  microbial  rot,  both  of  which  reduce  sucrose  content  and  quality.  Cercospora  leaf  spot  (CLS)  has  been  considered  a  potential  predisposing  factor  for  increased  storage  rot.  In  the  current  study,  across  three  CLS-susceptible  commercial  varieties,  there  were  no  significant  differences  between  storage  rot  susceptibility  to  any  of  the  three  tested  pathogens  in  hand-harvested  sugarbeets,  regardless  of  CLS  level,  at  any  storage  timepoint  in  2020  or  2021  (P    0.05).  In  studies  using  CLS-susceptible  and  -resistant  germplasm  and  varieties,  CLS  effects  were  inconsistent  and  only  significant  in  one  parameter  at  two  of  six  storage  timepoints  across  years  (P    0.05).  With  the  tested  storage  pathogens,  prior  CLS  level  also  did  not  impact  root  respiration  or  the  change  in  respiration  rate  from  initial  to  final  storage  timepoint  in  either  2021  or  2023  (P    0.05).  These  results  showed  that  foliar  CLS  infection  alone  may  not  predispose  beets  to  storage  rot.  Mechanical  harvest,  on  the  other  hand,  substantially  increased  disease  severity  across  all  treatments,  confirming  that  physical  damage  is  a  more  critical  factor  in  postharvest  rot  than  in-season  foliar  stress.Commercial  storage  facilities  continue  to  stress  anecdotal  evidence  of  CLS  impacting  storability  of  sugar  beets.  To  further  investigate  the  impact  of  CLS  on  commercial  storage,  post-harvest  symptom  development  was  evaluated  in  bruised  beets  with  relatively  high  or  low  in-season  CLS  severity  in  2022  and  2023.  Bruised  and  non-bruised  tissue  of  roots  of  each  CLS  level  were  inoculated  with  known  storage  pathogens,  and  while  bruising  did  significantly  impact  rot  development  at  all  timepoints  (P    0.05),  CLS  did  not  impact  rate  of  rot  development  in  bruised  beets.To  inform  future  breeding  efforts,  twelve  commercial  sugarbeet  varieties  were  evaluated  for  their  susceptibility  to  common  storage  rot  pathogens  across  two  storage  seasons;  Botrytis  cinerea,  Fusarium  graminearum,  and  Penicillium  vulpinum  across  two  storage  seasons.  Roots  were  harvested  in  2022  and  2023,  sliced,  and  inoculated  under  controlled  storage  conditions.  All  varieties  exhibited  susceptibility  to  the  pathogens,  with  B.  cinerea  consistently  inducing  the  most  severe  rot.  No  variety  demonstrated  consistent  resistance  across  both  years  and  all  pathogens.  Interestingly,  several  varieties  that  showed  minimal  rot  under  hand-harvest  conditions  in  2022  developed  significantly  more  severe  symptoms  following  machine  harvest  in  2023,  suggesting  that  mechanical  damage  may  have  a  greater  influence  on  rot  development  than  varietal  resistance  alone.  These  findings  highlight  the  need  for  broader  screening  efforts  that  incorporate  diverse  pathogen  populations,  harvest  methods,  and  more  representative  storage  conditions.  Breeding  for  postharvest  resistance  remains  a  largely  untapped  avenue  with  strong  potential  to  reduce  losses  and  enhance  the  long-term  storability  of  sugarbeet  crops.A  multi-year  pathogen  survey  to  identify  the  fungal  organisms  most  commonly  associated  with  storage  rot  in  Michigan  was  conducted  from  2020-2022  and  in  2024.  A  focus  on  Penicillium  spp.  and  Fusarium  spp.  collected  in  2024  were  further  identified  using  DNA  sequencing.  Molecular  analysis  identified  five  primary  Fusarium  species:  F.  oxysporum,  F.  fujikuroi,  F.  solani,  F.  graminearum,  and  F.  acuminatum,  as  well  as  one  isolate  of  F.  tricinctum.  Penicillium  isolates  included  P.  expansum,  P.  polonicum,  P.  crustosum,  P.  paneum,  and  P.  brasilianum.  These  findings  emphasize  that  sugarbeet  storage  rot  in  Michigan  is  caused  by  a  diverse,  changing  fungal  community  and  that  pathogen  levels  and  type  may  vary  by  year  and  site.Together,  this  research  highlights  the  importance  of  both  identifying  storage  pathogens  and  understanding  how  field  and  postharvest  factors  interact  to  affect  disease  severity.  This  research  contributes  to  ongoing  efforts  to  reduce  postharvest  losses,  enhance  sugar  yield,  and  support  more  sustainable  sugarbeet  production  systems.
■590    ▼aSchool  code:  0128.
■650  4▼aPlant  pathology
■650  4▼aMicrobiology
■650  4▼aPlant  sciences
■650  4▼aPathology
■653    ▼aCercospora  leaf  spot
■653    ▼aPathogens
■653    ▼aStorage  rot
■653    ▼aSugarbeet
■653    ▼aMichigan
■690    ▼a0480
■690    ▼a0410
■690    ▼a0479
■690    ▼a0571
■71020▼aMichigan  State  University▼bPlant  Pathology  -  Doctor  of  Philosophy.
■7730  ▼tDissertations  Abstracts  International▼g87-05B.
■790    ▼a0128
■791    ▼aPh.D.
■792    ▼a2025
■793    ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17360112▼nKERIS▼z이  자료의  원문은  한국교육학술정보원에서  제공합니다.

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