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Nanotechnologies for Single-Molecule Manipulation and Measurement
Nanotechnologies for Single-Molecule Manipulation and Measurement
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105308
- ISBN
- 9798273307186
- DDC
- 574.191
- 저자명
- Hong, Yifeng.
- 서명/저자
- Nanotechnologies for Single-Molecule Manipulation and Measurement
- 발행사항
- [Sl] : Cornell University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 156 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-07, Section: B.
- 주기사항
- Advisor: Wang, Michelle.
- 학위논문주기
- Thesis (Ph.D.)--Cornell University, 2025.
- 초록/해제
- 요약Optical tweezer is a cornerstone technique for biophysical research, enabling direct investigation of DNA-based processes at the single-molecule level. Advances in single-molecule optical tweezer assays have largely facilitated understanding of the mechanical properties of DNA and its interactions with proteins, which is essential to elucidate fundamental biological processes such as transcription and replication. This dissertation presents the development of advanced nanotechnologies along with their applications that extend the capabilities of single-molecule manipulation and measurement.First, a resonator-enhanced nanophotonic standing-wave array trap (nSWAT) is engineered to achieve parallel, high-force optical trapping on a photonic chip. This device enables simultaneous measurements of DNA elastic properties and protein-DNA interactions with base-pair resolution. Second, a comprehensive optical torque simulation framework is established for the angular optical trap (AOT). This framework enables systematic modeling and designing of an AOT trapping particle. Third, torsional measurements on braided DNA using the quartz cylinder on the AOT reveal how geometrical constraints dictated supercoiling partitioning during replication. Finally, a new class of biocompatible metamaterial elliptical cylinders is designed and fabricated as high-rotation-speed and high-torque-resolution probes for the AOT. This new class of AOT trapping particle opens opportunities for previously inaccessible rotational studies of DNA-based processes.Together, these works advance the frontier of single-molecule manipulation and measurement technologies, providing versatile and high-performance tools for quantitative investigations of roadblocks and topological challenges in DNA-based processes.
- 일반주제명
- Biophysics
- 일반주제명
- Optics
- 일반주제명
- Nanotechnology
- 일반주제명
- Molecular biology
- 키워드
- Optical tweezer
- 기타저자
- Cornell University Electrical and Computer Engineering
- 기본자료저록
- Dissertations Abstracts International. 87-07B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520260202105308
■006m o d
■007cr#unu||||||||
■020 ▼a9798273307186
■035 ▼a(MiAaPQ)AAI32283834
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574.191
■1001 ▼aHong, Yifeng.
■24510▼aNanotechnologies for Single-Molecule Manipulation and Measurement
■260 ▼a[Sl]▼bCornell University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a156 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-07, Section: B.
■500 ▼aAdvisor: Wang, Michelle.
■5021 ▼aThesis (Ph.D.)--Cornell University, 2025.
■520 ▼aOptical tweezer is a cornerstone technique for biophysical research, enabling direct investigation of DNA-based processes at the single-molecule level. Advances in single-molecule optical tweezer assays have largely facilitated understanding of the mechanical properties of DNA and its interactions with proteins, which is essential to elucidate fundamental biological processes such as transcription and replication. This dissertation presents the development of advanced nanotechnologies along with their applications that extend the capabilities of single-molecule manipulation and measurement.First, a resonator-enhanced nanophotonic standing-wave array trap (nSWAT) is engineered to achieve parallel, high-force optical trapping on a photonic chip. This device enables simultaneous measurements of DNA elastic properties and protein-DNA interactions with base-pair resolution. Second, a comprehensive optical torque simulation framework is established for the angular optical trap (AOT). This framework enables systematic modeling and designing of an AOT trapping particle. Third, torsional measurements on braided DNA using the quartz cylinder on the AOT reveal how geometrical constraints dictated supercoiling partitioning during replication. Finally, a new class of biocompatible metamaterial elliptical cylinders is designed and fabricated as high-rotation-speed and high-torque-resolution probes for the AOT. This new class of AOT trapping particle opens opportunities for previously inaccessible rotational studies of DNA-based processes.Together, these works advance the frontier of single-molecule manipulation and measurement technologies, providing versatile and high-performance tools for quantitative investigations of roadblocks and topological challenges in DNA-based processes.
■590 ▼aSchool code: 0058.
■650 4▼aBiophysics
■650 4▼aOptics
■650 4▼aNanotechnology
■650 4▼aMolecular biology
■653 ▼aOptical tweezer
■653 ▼aSingle-molecule manipulation
■653 ▼aAngular optical trap
■653 ▼aNanophotonic standing-wave array trap
■653 ▼aSupercoiling partitioning
■690 ▼a0786
■690 ▼a0752
■690 ▼a0652
■690 ▼a0307
■71020▼aCornell University▼bElectrical and Computer Engineering.
■7730 ▼tDissertations Abstracts International▼g87-07B.
■790 ▼a0058
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17360127▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
