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Mechanics and Energetics of Cytoskeletal Assembly
Mechanics and Energetics of Cytoskeletal Assembly
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103035
- ISBN
- 9798286445080
- DDC
- 530
- 서명/저자
- Mechanics and Energetics of Cytoskeletal Assembly
- 발행사항
- [Sl] : Yale University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 246 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
- 주기사항
- Advisor: Murrell, Michael P.
- 학위논문주기
- Thesis (Ph.D.)--Yale University, 2025.
- 초록/해제
- 요약Active matter is a subfield of soft condensed matter physics that focuses on the emergence of collective phenomena, as components of the system consume energy, driving them out of thermodynamic equilibrium. Living matter, a specific subfield of active matter, lies between physics and biology. In this case, the components are biomolecules, whose interactions are not only constrained by the laws of physics but may exhibit purpose and intentionality. Biophysicists, who study living matter use reductionist approaches and focus on how different components of the system can collectively give rise to novel biological phenomena through continuous consumption of energy.An example of a driven, non-equilibrium thermodynamic system is the cell cytoskeleton, which is an exquisite machinery that is responsible for complex tasks such as migration and division. It is composed of proteins and other biological macromolecules which interact to yield collective behaviors. For example, the protein scaffolding of the cell (F-actin) undergoes dramatic changes in structure, organization, and dynamics through impartation of mechanical stresses by molecular motors (myosin) which consume chemical energy during these morphological changes. However, how the consumption of chemical energy drives the mechanical performance of biological, protein-based machines is unknown. By using cutting edge biophysical experimental techniques and tools such as confocal microscopy, (micro)rheology, and pico-calorimetry, this thesis examines phenomena across scales from molecular level energetic consumption and dissipation to large micrometer-level collective and critical phenomena. We use the bottom-up approach and assemble the biomimetic cytoskeletal structure, the actomyosin network, using minimalistic purified protein components (in vitro).In this thesis, I will summarize several aspects related to the active mechanics and energetics of the cytoskeletal assembly such as how material properties relate to chemical consumption and vice versa. In doing so, I will describe unique aspects of non-equilibrium materials, including active stress propagation, energy consumption and dissipation, self-tuned critical behaviors, novel constitutive relationships, emergence of spatiotemporal instabilities (in vitro actin waves), and the change in heat dissipation and energetic efficiency.From these findings, we offer novel insights on the cytoskeleton as an auto-feedback network system that uses mechanochemical and biomechanical cues to regulate mechanical information propagation as well as the bioenergetic properties of the cytoskeleton as a crucial part of the machinery. We challenge the current understanding of the cytoskeleton field from a more network-focused perspective and motivate the investigation of how a cell regulates its energetic efficiency as an out-of-equilibrium thermodynamical machine.
- 일반주제명
- Physics
- 일반주제명
- Biophysics
- 일반주제명
- Thermodynamics
- 일반주제명
- Molecular biology
- 키워드
- Active matter
- 키워드
- Complex systems
- 키워드
- Living matter
- 키워드
- Rheology
- 기타저자
- Yale University Physics
- 기본자료저록
- Dissertations Abstracts International. 86-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■006m o d
■007cr#unu||||||||
■020 ▼a9798286445080
■035 ▼a(MiAaPQ)AAI31846251
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a530
■1001 ▼aSun, Zachary Gao.
■24510▼aMechanics and Energetics of Cytoskeletal Assembly
■260 ▼a[Sl]▼bYale University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a246 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-12, Section: B.
■500 ▼aAdvisor: Murrell, Michael P.
■5021 ▼aThesis (Ph.D.)--Yale University, 2025.
■520 ▼aActive matter is a subfield of soft condensed matter physics that focuses on the emergence of collective phenomena, as components of the system consume energy, driving them out of thermodynamic equilibrium. Living matter, a specific subfield of active matter, lies between physics and biology. In this case, the components are biomolecules, whose interactions are not only constrained by the laws of physics but may exhibit purpose and intentionality. Biophysicists, who study living matter use reductionist approaches and focus on how different components of the system can collectively give rise to novel biological phenomena through continuous consumption of energy.An example of a driven, non-equilibrium thermodynamic system is the cell cytoskeleton, which is an exquisite machinery that is responsible for complex tasks such as migration and division. It is composed of proteins and other biological macromolecules which interact to yield collective behaviors. For example, the protein scaffolding of the cell (F-actin) undergoes dramatic changes in structure, organization, and dynamics through impartation of mechanical stresses by molecular motors (myosin) which consume chemical energy during these morphological changes. However, how the consumption of chemical energy drives the mechanical performance of biological, protein-based machines is unknown. By using cutting edge biophysical experimental techniques and tools such as confocal microscopy, (micro)rheology, and pico-calorimetry, this thesis examines phenomena across scales from molecular level energetic consumption and dissipation to large micrometer-level collective and critical phenomena. We use the bottom-up approach and assemble the biomimetic cytoskeletal structure, the actomyosin network, using minimalistic purified protein components (in vitro).In this thesis, I will summarize several aspects related to the active mechanics and energetics of the cytoskeletal assembly such as how material properties relate to chemical consumption and vice versa. In doing so, I will describe unique aspects of non-equilibrium materials, including active stress propagation, energy consumption and dissipation, self-tuned critical behaviors, novel constitutive relationships, emergence of spatiotemporal instabilities (in vitro actin waves), and the change in heat dissipation and energetic efficiency.From these findings, we offer novel insights on the cytoskeleton as an auto-feedback network system that uses mechanochemical and biomechanical cues to regulate mechanical information propagation as well as the bioenergetic properties of the cytoskeleton as a crucial part of the machinery. We challenge the current understanding of the cytoskeleton field from a more network-focused perspective and motivate the investigation of how a cell regulates its energetic efficiency as an out-of-equilibrium thermodynamical machine.
■590 ▼aSchool code: 0265.
■650 4▼aPhysics
■650 4▼aBiophysics
■650 4▼aThermodynamics
■650 4▼aMolecular biology
■653 ▼aActive matter
■653 ▼aComplex systems
■653 ▼aLiving matter
■653 ▼aRheology
■653 ▼aSelf-organized criticality
■690 ▼a0605
■690 ▼a0786
■690 ▼a0348
■690 ▼a0307
■71020▼aYale University▼bPhysics.
■7730 ▼tDissertations Abstracts International▼g86-12B.
■790 ▼a0265
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17356789▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
