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Collective Behaviors in Exogenously Controlled Epithelia
Collective Behaviors in Exogenously Controlled Epithelia
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103514
- ISBN
- 9798280748415
- DDC
- 574.191
- 서명/저자
- Collective Behaviors in Exogenously Controlled Epithelia
- 발행사항
- [Sl] : Princeton University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 116 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
- 주기사항
- Advisor: Cohen, Daniel J.
- 학위논문주기
- Thesis (Ph.D.)--Princeton University, 2025.
- 초록/해제
- 요약Mammalian cells leverage collective behaviors to coordinate movement, force production, and fluid pumping during a range of crucial biological processes. These collective behaviors are essential for establishing and maintaining homeostasis and require constitutive cells to accurately sense and respond to their environment. Whereas these collective behaviors are relatively well-studied in the context of confined and freely migrating mature epithelia, it remains poorly understood how they are modulated by exogenous cues. The work presented in this dissertation addresses this gap in knowledge by directly measuring collective behaviors in exogenously controlled epithelial tissues. First, we present data showing that short-term bioelectric stimulation of an epithelium has longlasting effects on collective migration in the system. We explore the spatiotemporal dynamics of migrational speed, alignment, and correlation in distinct regions of the tissue before, during, and after bioelectric stimulation. These data show that epithelia exhibit an inhomogeneous response to a homogeneous cue. We then show that bioelectric stimulation can be used in a 3D context to control fluid pumping and migration in a lab-grown kidney model via a process we call 'electro-inflation'. We performed inhibition assays and developed a continuum model to show that electro-inflation is driven by ion crowding and mediated by a balance between ion channel activity and cytoskeletal mechanics. We then generalize our study of collective behavior to exogenous cues outside of bioelectricity by presenting a bioengineered system wherein cells are forced to adhere to their substrate via cadherins - a family of proteins used exclusively in cell-cell junctions. We show that this bio-inspired functionalized system directly modulates force propagation, migrational state, and cell-cycling in the epithelium. Finally, we present ongoing work on mapping the spatiotemporal expenditure of mechanical energy in epithelia using a combination of traction force microscopy and modeling. We show that energy expenditure in epithelia is spatiotemporally patterned, scales with tissue size, and actively regulates migration. Broadly, this dissertation presents a suite of analytical and experimental methods for studying epithelial collective behavior in perturbed environments and data that may prove useful for translating bioengineering techniques such as bioelectric stimulation and surface functionalization to medical instrumentation.
- 일반주제명
- Biophysics
- 일반주제명
- Biomechanics
- 일반주제명
- Bioengineering
- 일반주제명
- Cellular biology
- 키워드
- Mammalian cells
- 키워드
- Fluid pumping
- 기타저자
- Princeton University Quantitative Computational Biology
- 기본자료저록
- Dissertations Abstracts International. 86-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■007cr#unu||||||||
■020 ▼a9798280748415
■035 ▼a(MiAaPQ)AAI32037961
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574.191
■1001 ▼aBreinyn, Isaac B.▼0(orcid)0000-0002-4831-8398
■24510▼aCollective Behaviors in Exogenously Controlled Epithelia
■260 ▼a[Sl]▼bPrinceton University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a116 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-12, Section: B.
■500 ▼aAdvisor: Cohen, Daniel J.
■5021 ▼aThesis (Ph.D.)--Princeton University, 2025.
■520 ▼aMammalian cells leverage collective behaviors to coordinate movement, force production, and fluid pumping during a range of crucial biological processes. These collective behaviors are essential for establishing and maintaining homeostasis and require constitutive cells to accurately sense and respond to their environment. Whereas these collective behaviors are relatively well-studied in the context of confined and freely migrating mature epithelia, it remains poorly understood how they are modulated by exogenous cues. The work presented in this dissertation addresses this gap in knowledge by directly measuring collective behaviors in exogenously controlled epithelial tissues. First, we present data showing that short-term bioelectric stimulation of an epithelium has longlasting effects on collective migration in the system. We explore the spatiotemporal dynamics of migrational speed, alignment, and correlation in distinct regions of the tissue before, during, and after bioelectric stimulation. These data show that epithelia exhibit an inhomogeneous response to a homogeneous cue. We then show that bioelectric stimulation can be used in a 3D context to control fluid pumping and migration in a lab-grown kidney model via a process we call 'electro-inflation'. We performed inhibition assays and developed a continuum model to show that electro-inflation is driven by ion crowding and mediated by a balance between ion channel activity and cytoskeletal mechanics. We then generalize our study of collective behavior to exogenous cues outside of bioelectricity by presenting a bioengineered system wherein cells are forced to adhere to their substrate via cadherins - a family of proteins used exclusively in cell-cell junctions. We show that this bio-inspired functionalized system directly modulates force propagation, migrational state, and cell-cycling in the epithelium. Finally, we present ongoing work on mapping the spatiotemporal expenditure of mechanical energy in epithelia using a combination of traction force microscopy and modeling. We show that energy expenditure in epithelia is spatiotemporally patterned, scales with tissue size, and actively regulates migration. Broadly, this dissertation presents a suite of analytical and experimental methods for studying epithelial collective behavior in perturbed environments and data that may prove useful for translating bioengineering techniques such as bioelectric stimulation and surface functionalization to medical instrumentation.
■590 ▼aSchool code: 0181.
■650 4▼aBiophysics
■650 4▼aBiomechanics
■650 4▼aBioengineering
■650 4▼aCellular biology
■653 ▼aMammalian cells
■653 ▼aCollective behaviors
■653 ▼aExogenously controlled epithelial tissues
■653 ▼aFluid pumping
■690 ▼a0786
■690 ▼a0648
■690 ▼a0202
■690 ▼a0379
■71020▼aPrinceton University▼bQuantitative Computational Biology.
■7730 ▼tDissertations Abstracts International▼g86-12B.
■790 ▼a0181
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357454▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
