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Cooperative Functions of the Microtubule and Actin Cytoskeletons for Cell Division in the Flowering Plant A. thaliana
Cooperative Functions of the Microtubule and Actin Cytoskeletons for Cell Division in the Flowering Plant A. thaliana
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103618
- ISBN
- 9798290614298
- DDC
- 580
- 서명/저자
- Cooperative Functions of the Microtubule and Actin Cytoskeletons for Cell Division in the Flowering Plant A. thaliana
- 발행사항
- [Sl] : University of California, Davis, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 112 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-01, Section: B.
- 주기사항
- Advisor: Liu, Bo.
- 학위논문주기
- Thesis (Ph.D.)--University of California, Davis, 2025.
- 초록/해제
- 요약Cell division is a process that ensures faithful segregation of replicated genetic materials followed by partitioning of the cytoplasm. In all eukaryotes, the process of cell division is dependent on the dynamic cytoskeleton. In plants, the two major protein polymers that constitute the cytoskeleton are microtubules and F-actin. During cell division, the cytoskeleton is reorganized into different arrays of different physiological functions. These arrays include the preprophase band, spindle and phragmoplast, which function to predict the future division plane, segregate chromosomes, and form the cell plate, respectively. In flowering plants, these microtubule-based structures are nucleated without a structurally defined microtubule-organizing center like the centrosome. So, an intriguing question has been how these arrays, especially those of the spindle, are organized in the absence of centrosomes. Furthermore, a network of F-actin is also detected by being incorporated into the microtubule-based apparatuses and disruption of either the F-actin or microtubules can lead to defects in cell division. Earlier studies raise a question of how different cytoskeleton filaments can interact or crosstalk to execute faithful cell division in acentrosomal cells. Here, we reveal novel functions of microtubule motors, kinesins, and F-actin motors, myosins, in regulating cell division and detected their physical interaction for such functions.This dissertation uncovers new roles for cytoskeletal motor proteins, microtubule-based kinesins and F-actin-based myosins, in coordinating plant cell division. In Chapter 1, I identify a previously uncharacterized subclass of Kinesin-14 motors (class D) in Arabidopsis thaliana that localizes to the spindle midzone and is essential for organizing kinetochore fibers during spindle formation. Chapter 2 explores the function of Myosin XI motors in cytokinesis, revealing that the Myosin XI motor protein, MYA1, localizes to the cortical division site (CDS) in a preprophase band-dependent manner. MYA1 interacts with other CDS proteins, including the microtubule-associated protein TANGLED1 and the Kinesin-12 motor Phragmoplast Orienting Kinesin 1 (POK1). Together, these proteins form a higher-order complex or assemblies, which we term Cytoskeleton-Associated Motor assemblies at the PPB site (CAMP), that coordinates phragmoplast expansion and ensures precise cell plate positioning.Together, these findings highlight how coordinated actions of microtubule and actin motors ensure faithful cell division in acentrosomal plant cells.
- 일반주제명
- Botany
- 일반주제명
- Cellular biology
- 일반주제명
- Plant sciences
- 키워드
- Actin
- 키워드
- Kinesin-14
- 키워드
- Myosin XI
- 기타저자
- University of California, Davis Plant Biology
- 기본자료저록
- Dissertations Abstracts International. 87-01B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520260202103618
■006m o d
■007cr#unu||||||||
■020 ▼a9798290614298
■035 ▼a(MiAaPQ)AAI32045081
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a580
■1001 ▼aHuang, Calvin Haoyuan.
■24510▼aCooperative Functions of the Microtubule and Actin Cytoskeletons for Cell Division in the Flowering Plant A. thaliana
■260 ▼a[Sl]▼bUniversity of California, Davis▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a112 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-01, Section: B.
■500 ▼aAdvisor: Liu, Bo.
■5021 ▼aThesis (Ph.D.)--University of California, Davis, 2025.
■520 ▼aCell division is a process that ensures faithful segregation of replicated genetic materials followed by partitioning of the cytoplasm. In all eukaryotes, the process of cell division is dependent on the dynamic cytoskeleton. In plants, the two major protein polymers that constitute the cytoskeleton are microtubules and F-actin. During cell division, the cytoskeleton is reorganized into different arrays of different physiological functions. These arrays include the preprophase band, spindle and phragmoplast, which function to predict the future division plane, segregate chromosomes, and form the cell plate, respectively. In flowering plants, these microtubule-based structures are nucleated without a structurally defined microtubule-organizing center like the centrosome. So, an intriguing question has been how these arrays, especially those of the spindle, are organized in the absence of centrosomes. Furthermore, a network of F-actin is also detected by being incorporated into the microtubule-based apparatuses and disruption of either the F-actin or microtubules can lead to defects in cell division. Earlier studies raise a question of how different cytoskeleton filaments can interact or crosstalk to execute faithful cell division in acentrosomal cells. Here, we reveal novel functions of microtubule motors, kinesins, and F-actin motors, myosins, in regulating cell division and detected their physical interaction for such functions.This dissertation uncovers new roles for cytoskeletal motor proteins, microtubule-based kinesins and F-actin-based myosins, in coordinating plant cell division. In Chapter 1, I identify a previously uncharacterized subclass of Kinesin-14 motors (class D) in Arabidopsis thaliana that localizes to the spindle midzone and is essential for organizing kinetochore fibers during spindle formation. Chapter 2 explores the function of Myosin XI motors in cytokinesis, revealing that the Myosin XI motor protein, MYA1, localizes to the cortical division site (CDS) in a preprophase band-dependent manner. MYA1 interacts with other CDS proteins, including the microtubule-associated protein TANGLED1 and the Kinesin-12 motor Phragmoplast Orienting Kinesin 1 (POK1). Together, these proteins form a higher-order complex or assemblies, which we term Cytoskeleton-Associated Motor assemblies at the PPB site (CAMP), that coordinates phragmoplast expansion and ensures precise cell plate positioning.Together, these findings highlight how coordinated actions of microtubule and actin motors ensure faithful cell division in acentrosomal plant cells.
■590 ▼aSchool code: 0029.
■650 4▼aBotany
■650 4▼aCellular biology
■650 4▼aPlant sciences
■653 ▼aAcentrosomal spindle
■653 ▼aActin
■653 ▼aDivision site determination
■653 ▼aInterpolar microtubules
■653 ▼aKinesin-14
■653 ▼aMyosin XI
■690 ▼a0309
■690 ▼a0379
■690 ▼a0479
■71020▼aUniversity of California, Davis▼bPlant Biology.
■7730 ▼tDissertations Abstracts International▼g87-01B.
■790 ▼a0029
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357925▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
