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New Platforms to Expand the Detection Repertoire of Cell-Free Biosensors
New Platforms to Expand the Detection Repertoire of Cell-Free Biosensors
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105521
- ISBN
- 9798263342524
- DDC
- 000
- 서명/저자
- New Platforms to Expand the Detection Repertoire of Cell-Free Biosensors
- 발행사항
- [Sl] : Georgia Institute of Technology, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 189 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-05, Section: B.
- 주기사항
- Advisor: Styczynski, Mark P.
- 학위논문주기
- Thesis (Ph.D.)--Georgia Institute of Technology, 2024.
- 초록/해제
- 요약Accurate measurement of biomarkers is critical for properly assessing and managing disease, but standard methods for biomarker quantification involve laborintensive protocols and expensive equipment that make disease diagnosis difficult in resource-limited settings. The development of rapid, sensitive, and specific sensors that can be used at the point of care could increase testing accessibility in low-resource areas and have an immense impact on global health. While cell-free synthetic biology allows us to design powerful yet accessible biosensors, the range of detectable analytes is limited due to constraints in existing sensor frameworks.This thesis describes my work in developing new platforms to broaden the range of detectable analytes for cell-free biosensors. Specifically, I engineered the first highly adaptable biosensing platform for low-cost protein detection. I also broadened the application space of RNA toehold switch activators and repressors to include shorter RNA inputs. These findings have a strong potential impact in microRNA sensing applications, a space where cell-free expression systems previously had little footprint. Lastly, I describe progress towards a revolutionary new biosensing platform that couples structure-switching DNA aptamers with RNA toehold switches. This technology would enable biosensor construction for virtually any biomarker. Taken together, this work presents several advances in cell-free biosensors that make it possible to detect previously undetectable analytes.
- 일반주제명
- MicroRNAs
- 일반주제명
- Plasmids
- 일반주제명
- Biomarkers
- 일반주제명
- Design
- 일반주제명
- Biochemistry
- 기본자료저록
- Dissertations Abstracts International. 87-05B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798263342524
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■035 ▼a(MiAaPQ)GeorgiaTech77672
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a000
■1001 ▼aMcSweeney, Megan Ashley.
■24510▼aNew Platforms to Expand the Detection Repertoire of Cell-Free Biosensors
■260 ▼a[Sl]▼bGeorgia Institute of Technology▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a189 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-05, Section: B.
■500 ▼aAdvisor: Styczynski, Mark P.
■5021 ▼aThesis (Ph.D.)--Georgia Institute of Technology, 2024.
■520 ▼aAccurate measurement of biomarkers is critical for properly assessing and managing disease, but standard methods for biomarker quantification involve laborintensive protocols and expensive equipment that make disease diagnosis difficult in resource-limited settings. The development of rapid, sensitive, and specific sensors that can be used at the point of care could increase testing accessibility in low-resource areas and have an immense impact on global health. While cell-free synthetic biology allows us to design powerful yet accessible biosensors, the range of detectable analytes is limited due to constraints in existing sensor frameworks.This thesis describes my work in developing new platforms to broaden the range of detectable analytes for cell-free biosensors. Specifically, I engineered the first highly adaptable biosensing platform for low-cost protein detection. I also broadened the application space of RNA toehold switch activators and repressors to include shorter RNA inputs. These findings have a strong potential impact in microRNA sensing applications, a space where cell-free expression systems previously had little footprint. Lastly, I describe progress towards a revolutionary new biosensing platform that couples structure-switching DNA aptamers with RNA toehold switches. This technology would enable biosensor construction for virtually any biomarker. Taken together, this work presents several advances in cell-free biosensors that make it possible to detect previously undetectable analytes.
■590 ▼aSchool code: 0078.
■650 4▼aMicroRNAs
■650 4▼aPlasmids
■650 4▼aBiomarkers
■650 4▼aDesign
■650 4▼aBiochemistry
■690 ▼a0389
■690 ▼a0487
■71020▼aGeorgia Institute of Technology.
■7730 ▼tDissertations Abstracts International▼g87-05B.
■790 ▼a0078
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17360413▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
