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Interrogation of Intermolecular Interactions in Liquid-Liquid Phase Separation
Interrogation of Intermolecular Interactions in Liquid-Liquid Phase Separation
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202104824
- ISBN
- 9798290910611
- DDC
- 540
- 저자명
- Xu, Ruiwen.
- 서명/저자
- Interrogation of Intermolecular Interactions in Liquid-Liquid Phase Separation
- 발행사항
- [Sl] : The University of Wisconsin - Madison, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 264 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-01, Section: B.
- 주기사항
- Advisor: Gellman, Samuel H.
- 학위논문주기
- Thesis (Ph.D.)--The University of Wisconsin - Madison, 2025.
- 초록/해제
- 요약Liquid-liquid phase separation (LLPS) plays an important role in many cellular processes. The RNA-binding protein Fused in Sarcoma (FUS) has been shown to mediate phase separation through attractive interactions between the tyrosine-rich N-terminal domain and the arginine-rich C-terminal domain. Cation-π interactions between Arg and Tyr side chains have been proposed as a driving force for condensed phase formation involving FUS and related proteins. To explore this hypothesis, we developed a two-component system comprising a long Arg-rich FUS fragment, generated via heterologous expression, and a short Tyr-rich peptide, generated via chemical synthesis. Phase separation occurs when these components are mixed at low concentrations (5 μM each). Global replacement of Tyr with noncanonical residues allowed us to interrogate the role of the phenolic side chain in driving the polypeptide assembly that underlies condensed phase formation. The results suggest that cation-π interactions do not make a major contribution to inter- peptide attractions in our model system. Instead, the H-bond donor properties of the Tyr side chain hydroxyl appear to play a central role in driving phase separation.
- 일반주제명
- Chemistry
- 일반주제명
- Cellular biology
- 일반주제명
- Biochemistry
- 일반주제명
- Genetics
- 키워드
- Fused in Sarcoma
- 키워드
- Phase separation
- 기타저자
- The University of Wisconsin - Madison Chemistry
- 기본자료저록
- Dissertations Abstracts International. 87-01B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■006m o d
■007cr#unu||||||||
■020 ▼a9798290910611
■035 ▼a(MiAaPQ)AAI32169675
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a540
■1001 ▼aXu, Ruiwen.
■24510▼aInterrogation of Intermolecular Interactions in Liquid-Liquid Phase Separation
■260 ▼a[Sl]▼bThe University of Wisconsin - Madison▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a264 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-01, Section: B.
■500 ▼aAdvisor: Gellman, Samuel H.
■5021 ▼aThesis (Ph.D.)--The University of Wisconsin - Madison, 2025.
■520 ▼aLiquid-liquid phase separation (LLPS) plays an important role in many cellular processes. The RNA-binding protein Fused in Sarcoma (FUS) has been shown to mediate phase separation through attractive interactions between the tyrosine-rich N-terminal domain and the arginine-rich C-terminal domain. Cation-π interactions between Arg and Tyr side chains have been proposed as a driving force for condensed phase formation involving FUS and related proteins. To explore this hypothesis, we developed a two-component system comprising a long Arg-rich FUS fragment, generated via heterologous expression, and a short Tyr-rich peptide, generated via chemical synthesis. Phase separation occurs when these components are mixed at low concentrations (5 μM each). Global replacement of Tyr with noncanonical residues allowed us to interrogate the role of the phenolic side chain in driving the polypeptide assembly that underlies condensed phase formation. The results suggest that cation-π interactions do not make a major contribution to inter- peptide attractions in our model system. Instead, the H-bond donor properties of the Tyr side chain hydroxyl appear to play a central role in driving phase separation.
■590 ▼aSchool code: 0262.
■650 4▼aChemistry
■650 4▼aCellular biology
■650 4▼aBiochemistry
■650 4▼aGenetics
■653 ▼aLiquid-liquid phase separation
■653 ▼aFused in Sarcoma
■653 ▼aCation-π interactions
■653 ▼aPhase separation
■653 ▼aHeterologous expression
■690 ▼a0485
■690 ▼a0379
■690 ▼a0487
■690 ▼a0369
■71020▼aThe University of Wisconsin - Madison▼bChemistry.
■7730 ▼tDissertations Abstracts International▼g87-01B.
■790 ▼a0262
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17359032▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
