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Small RNAs, Big Influence: Microbial sRNAs as Modulators of Host Behavior and Microbial Fitness
Small RNAs, Big Influence: Microbial sRNAs as Modulators of Host Behavior and Microbial Fitness
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202104837
- ISBN
- 9798293894734
- DDC
- 576
- 저자명
- Marogi, Jacob.
- 서명/저자
- Small RNAs, Big Influence: Microbial sRNAs as Modulators of Host Behavior and Microbial Fitness
- 발행사항
- [Sl] : Princeton University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 97 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-04, Section: B.
- 주기사항
- Advisor: Gitai, Zemer;Myhrvold, Cameron.
- 학위논문주기
- Thesis (Ph.D.)--Princeton University, 2025.
- 초록/해제
- 요약Trans-kingdom signaling enables microbes to influence host behavior and physiology through the secretion of molecular cues. Here, we investigate the role of bacterial small RNAs (sRNAs) as key mediators of trans-kingdom communication, focusing on the interactions between the pathogen Pseudomonas aeruginosa and model host organism Caenorhabditis elegans. While sRNAs are well-established regulators of bacterial gene expression, emerging evidence suggests they are also implicated in affecting host behavioral and cellular responses, making them potent molecular messengers across biological kingdoms.First, we investigated the P. aeruginosa sRNA, P11 and highlighted its regulation of nitrogen assimilation in P. aeruginosa. Metabolic profiling and behavioral assays revealed that P11 regulation of nitrogen assimilation produces a volatile metabolite, ammonia, that influences C. elegans attraction to P. aeruginosa over non-harmful food sources, such as Escherichia coli. Additional to its function as an indirect chemoattractant, P11-mediated regulation of nitrogen assimilation is important for P. aeruginosa fitness during infections. Together, these findings highlight multiple functions of a single sRNA and how it can influence both C. elegans attraction and pathogen fitness during host infections.Next, we described an innovative sRNA detection strategy to identify P11 in C. elegans exposed to P. aeruginosa. Previous work demonstrated that prolonged exposure and feeding of P. aeruginosa by C. elegans results in host acquisition of P11. Driven by neural and epigenetic mechanisms, C. elegans processing of P11 results in aversive behavioral responses to P. aeruginosa that is transmitted across generations. Due to their small size, rapid degradation, and secondary structures, sRNA detection is limited by standard RNA-sequencing or hybridization-based methods. To overcome these obstacles, we utilized a CRISPR-Cas13 based platform tailored to microbial sRNA detection. This system enables highly specific, amplification-free detection of P. aeruginosa sRNAs allowing for temporal resolution of sRNA presence during P. aeruginosa infection and C. elegans behavioral memory formation.Together, this work establishes a mechanistic link between bacterial sRNA regulation of metabolism and host behavioral decision-making and introduces an diagnostic application of Cas13 for detecting trans-kingdom sRNA signals. These findings contribute to our understanding of microbe-induced host behavioral plasticity and the potential of RNA-based tools to study host-microbe communication.
- 일반주제명
- Microbiology
- 일반주제명
- Pathology
- 키워드
- Small RNAs
- 기타저자
- Princeton University Molecular Biology
- 기본자료저록
- Dissertations Abstracts International. 87-04B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798293894734
■035 ▼a(MiAaPQ)AAI32171765
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a576
■1001 ▼aMarogi, Jacob.
■24510▼aSmall RNAs, Big Influence: Microbial sRNAs as Modulators of Host Behavior and Microbial Fitness
■260 ▼a[Sl]▼bPrinceton University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a97 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-04, Section: B.
■500 ▼aAdvisor: Gitai, Zemer;Myhrvold, Cameron.
■5021 ▼aThesis (Ph.D.)--Princeton University, 2025.
■520 ▼aTrans-kingdom signaling enables microbes to influence host behavior and physiology through the secretion of molecular cues. Here, we investigate the role of bacterial small RNAs (sRNAs) as key mediators of trans-kingdom communication, focusing on the interactions between the pathogen Pseudomonas aeruginosa and model host organism Caenorhabditis elegans. While sRNAs are well-established regulators of bacterial gene expression, emerging evidence suggests they are also implicated in affecting host behavioral and cellular responses, making them potent molecular messengers across biological kingdoms.First, we investigated the P. aeruginosa sRNA, P11 and highlighted its regulation of nitrogen assimilation in P. aeruginosa. Metabolic profiling and behavioral assays revealed that P11 regulation of nitrogen assimilation produces a volatile metabolite, ammonia, that influences C. elegans attraction to P. aeruginosa over non-harmful food sources, such as Escherichia coli. Additional to its function as an indirect chemoattractant, P11-mediated regulation of nitrogen assimilation is important for P. aeruginosa fitness during infections. Together, these findings highlight multiple functions of a single sRNA and how it can influence both C. elegans attraction and pathogen fitness during host infections.Next, we described an innovative sRNA detection strategy to identify P11 in C. elegans exposed to P. aeruginosa. Previous work demonstrated that prolonged exposure and feeding of P. aeruginosa by C. elegans results in host acquisition of P11. Driven by neural and epigenetic mechanisms, C. elegans processing of P11 results in aversive behavioral responses to P. aeruginosa that is transmitted across generations. Due to their small size, rapid degradation, and secondary structures, sRNA detection is limited by standard RNA-sequencing or hybridization-based methods. To overcome these obstacles, we utilized a CRISPR-Cas13 based platform tailored to microbial sRNA detection. This system enables highly specific, amplification-free detection of P. aeruginosa sRNAs allowing for temporal resolution of sRNA presence during P. aeruginosa infection and C. elegans behavioral memory formation.Together, this work establishes a mechanistic link between bacterial sRNA regulation of metabolism and host behavioral decision-making and introduces an diagnostic application of Cas13 for detecting trans-kingdom sRNA signals. These findings contribute to our understanding of microbe-induced host behavioral plasticity and the potential of RNA-based tools to study host-microbe communication.
■590 ▼aSchool code: 0181.
■650 4▼aMicrobiology
■650 4▼aPathology
■653 ▼aSmall RNAs
■653 ▼aPseudomonas aeruginosa
■653 ▼aCaenorhabditis elegans
■653 ▼aMicrobial fitness
■690 ▼a0410
■690 ▼a0571
■71020▼aPrinceton University▼bMolecular Biology.
■7730 ▼tDissertations Abstracts International▼g87-04B.
■790 ▼a0181
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17359117▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
