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Innovating Feeding Laying Hens with Alternative Feed Proteins and Bioactive Nutrients for Sustainable Egg Production and Global Food and Nutrition Security
Innovating Feeding Laying Hens with Alternative Feed Proteins and Bioactive Nutrients for ...
Innovating Feeding Laying Hens with Alternative Feed Proteins and Bioactive Nutrients for Sustainable Egg Production and Global Food and Nutrition Security

Detailed Information

자료유형  
 학위논문 서양
최종처리일시  
20260311091503.5
ISBN  
9798283139876
DDC  
612
저자명  
Ou, Keith Jiajie
서명/저자  
Innovating Feeding Laying Hens with Alternative Feed Proteins and Bioactive Nutrients for Sustainable Egg Production and Global Food and Nutrition Security / Keith Jiajie Ou
발행사항  
[Sl] : Cornell University, 2025
형태사항  
1 electronic resource (202 pages)
주기사항  
Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
주기사항  
Advisors: Lei, Xingen Committee members: Danko, Charles; O'Brien, Kimberly; Duan, Jingyue.
학위논문주기  
- Ph.D. : Cornell University, 2025.
초록/해제  
요약With the continuous growth of the global population and the corresponding increase in demand for animal-sourced foods, achieving sustainable production has become an urgent priority for the egg industry. Alternative feed proteins present promising potential to replace soybean meal, but further research is required to elucidate the full effects on animal health and egg production. Rich in bioactive nutrients such as omega-3 polyunsaturated fatty acids (n-3 PUFA), these ingredients also offer opportunities for egg biofortification with health-promoting nutrients. Therefore, the objectives of this research were to investigate the effects of innovative alternative protein feeding strategies in laying hen diets on animal health, egg production, and nutrient enrichment in eggs, as well as to evaluate the safety and metabolic impacts of consuming such biofortified eggs in laboratory mice.A series of laying hen experiments were conducted to examine the effects of dietary inclusions of hempseed meal alone or in combination with microalgae Nannochloropsis oceanica and black soldier fly larvae. The 10% hempseed meal supplementation, rich in omega-3 alpha-linolenic acid, increased (P 0.001) the egg yolk docosahexaenoic acid (DHA) content by 1.0-fold to 57.8 mg/egg compared with the control. The additions of 1.0% microalgae and 2.5% or 5.0% black soldier fly larvae did not further enhance egg n-3 PUFA levels but modulated (P 0.05) manure ammonia production relative to the hempseed meal only diet. Another laying hen study was conducted using microalgal DHA oil to further improve egg DHA and n-3 PUFA biofortification. Triple enrichment of DHA (16 mg/g yolk), 25-hydroxyvitamin D3 [25(OH)D3] (160 ng/g yolk) and astaxanthin (5.5 µg/yolk) was achieved by feeding laying hens 0.8% the DHA-rich microalgal oil, 200 ppb 25(OH)D3 supplement, and 0.16 ppm microalgal astaxanthin. Supplementing 0.8% microalgal DHA oil resulted in 160 mg DHA/egg, while co-supplementation with microalgal astaxanthin led to a 13% reductio in DHA enrichment. In a follow-up mouse study, consumption of the DHA-enriched yolks at the recommended dose led to hepatic DHA accumulation (up to 61%; P 0.001) without affecting plasma triacylglycerol concentrations compared to the control. However, consuming enriched yolks at 5-fold the recommended dose did not result in significant hepatic DHA deposition across diets. Another mouse study was performed to evaluate the effects of biofortified eggs with substantial 25(OH)D3 enrichment comparing to synthetic supplement under both healthy and obesity-inducing high-fat diet (HFD) conditions. Consuming 700 IU 25(OH)D3 per kg of feed from biofortified yolks were safe, and mice consumed the HFD with biofortified yolk exhibited 33.4% higher (P 0.01) plasma 25(OH)D3 level and upregulated kidney Cyp24a1 (P 0.01) and Vdr (P 0.05) mRNA expression compared to their HFD counterparts receiving synthetic 25(OH)D3 supplement.With comparable production performance, alternative feed proteins such as hempseed meal, microalgae, and insect protein, demonstrated strong potential to replace soybean meal at up to 50% in supporting sustainable egg production while concurrently produce health-promoting biofortified eggs with up to 160 mg of DHA and 140 IU of 25(OH)D3 per egg for public health nutrition.
언어주기  
English
일반주제명  
Animal sciences
일반주제명  
Nutrition
일반주제명  
Molecular biology
키워드  
Alternative feed protein
키워드  
Egg biofortification
키워드  
Laboratory mouse
키워드  
Laying hen
키워드  
Omega-3 polyunsaturated fatty acids
키워드  
Vitamin D
기타저자  
Cornell University Animal Science
기본자료저록  
Dissertations Abstracts International. 86-12B.
전자적 위치 및 접속  
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MARC

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■1001  ▼aOu,  Keith  Jiajie▼eauthor.▼0(orcid)0000-0002-5597-0333
■24510▼aInnovating  Feeding  Laying  Hens  with  Alternative  Feed  Proteins  and  Bioactive  Nutrients  for  Sustainable  Egg  Production  and  Global  Food  and  Nutrition  Security  ▼cKeith  Jiajie  Ou
■260    ▼a[Sl]▼bCornell  University▼c2025
■264  1▼aAnn  Arbor▼bProQuest  Dissertations  &  Theses▼c2025
■300    ▼a1  electronic  resource  (202  pages)
■336    ▼atext▼btxt▼2rdacontent
■337    ▼acomputer▼bc▼2rdamedia
■338    ▼aonline  resource▼bcr▼2rdacarrier
■500    ▼aSource:  Dissertations  Abstracts  International,  Volume:  86-12,  Section:  B.
■500    ▼aAdvisors:  Lei,  Xingen    Committee  members:  Danko,  Charles;  O'Brien,  Kimberly;  Duan,  Jingyue.
■5021  ▼bPh.D.▼cCornell  University▼d2025.
■520    ▼aWith  the  continuous  growth  of  the  global  population  and  the  corresponding  increase  in  demand  for  animal-sourced  foods,  achieving  sustainable  production  has  become  an  urgent  priority  for  the  egg  industry.  Alternative  feed  proteins  present  promising  potential  to  replace  soybean  meal,  but  further  research  is  required  to  elucidate  the  full  effects  on  animal  health  and  egg  production.  Rich  in  bioactive  nutrients  such  as  omega-3  polyunsaturated  fatty  acids  (n-3  PUFA),  these  ingredients  also  offer  opportunities  for  egg  biofortification  with  health-promoting  nutrients.  Therefore,  the  objectives  of  this  research  were  to  investigate  the  effects  of  innovative  alternative  protein  feeding  strategies  in  laying  hen  diets  on  animal  health,  egg  production,  and  nutrient  enrichment  in  eggs,  as  well  as  to  evaluate  the  safety  and  metabolic  impacts  of  consuming  such  biofortified  eggs  in  laboratory  mice.A  series  of  laying  hen  experiments  were  conducted  to  examine  the  effects  of  dietary  inclusions  of  hempseed  meal  alone  or  in  combination  with  microalgae  Nannochloropsis  oceanica  and  black  soldier  fly  larvae.  The  10%  hempseed  meal  supplementation,  rich  in  omega-3  alpha-linolenic  acid,  increased  (P    0.001)  the  egg  yolk  docosahexaenoic  acid  (DHA)  content  by  1.0-fold  to  57.8  mg/egg  compared  with  the  control.  The  additions  of  1.0%  microalgae  and  2.5%  or  5.0%  black  soldier  fly  larvae  did  not  further  enhance  egg  n-3  PUFA  levels  but  modulated  (P    0.05)  manure  ammonia  production  relative  to  the  hempseed  meal  only  diet.  Another  laying  hen  study  was  conducted  using  microalgal  DHA  oil  to  further  improve  egg  DHA  and  n-3  PUFA  biofortification.  Triple  enrichment  of  DHA  (16  mg/g  yolk),  25-hydroxyvitamin  D3  [25(OH)D3]  (160  ng/g  yolk)  and  astaxanthin  (5.5  µg/yolk)  was  achieved  by  feeding  laying  hens  0.8%  the  DHA-rich  microalgal  oil,  200  ppb  25(OH)D3  supplement,  and  0.16  ppm  microalgal  astaxanthin.  Supplementing  0.8%  microalgal  DHA  oil  resulted  in  160  mg  DHA/egg,  while  co-supplementation  with  microalgal  astaxanthin  led  to  a  13%  reductio  in  DHA  enrichment.  In  a  follow-up  mouse  study,  consumption  of  the  DHA-enriched  yolks  at  the  recommended  dose  led  to  hepatic  DHA  accumulation  (up  to  61%;  P    0.001)  without  affecting  plasma  triacylglycerol  concentrations  compared  to  the  control.  However,  consuming  enriched  yolks  at  5-fold  the  recommended  dose  did  not  result  in  significant  hepatic  DHA  deposition  across  diets.  Another  mouse  study  was  performed  to  evaluate  the  effects  of  biofortified  eggs  with  substantial  25(OH)D3  enrichment  comparing  to  synthetic  supplement  under  both  healthy  and  obesity-inducing  high-fat  diet  (HFD)  conditions.  Consuming  700  IU  25(OH)D3  per  kg  of  feed  from  biofortified  yolks  were  safe,  and  mice  consumed  the  HFD  with  biofortified  yolk  exhibited  33.4%  higher  (P    0.01)  plasma  25(OH)D3  level  and  upregulated  kidney  Cyp24a1  (P    0.01)  and  Vdr  (P    0.05)  mRNA  expression  compared  to  their  HFD  counterparts  receiving  synthetic  25(OH)D3  supplement.With  comparable  production  performance,  alternative  feed  proteins  such  as  hempseed  meal,  microalgae,  and  insect  protein,  demonstrated  strong  potential  to  replace  soybean  meal  at  up  to  50%  in  supporting  sustainable  egg  production  while  concurrently  produce  health-promoting  biofortified  eggs  with  up  to  160  mg  of  DHA  and  140  IU  of  25(OH)D3  per  egg  for  public  health  nutrition.
■546    ▼aEnglish
■590    ▼aSchool  code:  0058
■650  4▼aAnimal  sciences
■650  4▼aNutrition
■650  4▼aMolecular  biology
■653    ▼aAlternative  feed  protein
■653    ▼aEgg  biofortification
■653    ▼aLaboratory  mouse
■653    ▼aLaying  hen
■653    ▼aOmega-3  polyunsaturated  fatty  acids
■653    ▼aVitamin  D
■7102  ▼aCornell  University▼bAnimal  Science.▼edegree  granting  institution.
■7201  ▼aLei,  Xingen▼edegree  supervisor.
■7730  ▼tDissertations  Abstracts  International▼g86-12B.
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357201▼nKERIS▼z이  자료의  원문은  한국교육학술정보원에서  제공합니다.

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