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Innovating Feeding Laying Hens with Alternative Feed Proteins and Bioactive Nutrients for Sustainable Egg Production and Global Food and Nutrition Security
Innovating Feeding Laying Hens with Alternative Feed Proteins and Bioactive Nutrients for Sustainable Egg Production and Global Food and Nutrition Security
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260311091503.5
- ISBN
- 9798283139876
- DDC
- 612
- 저자명
- Ou, Keith Jiajie
- 서명/저자
- Innovating Feeding Laying Hens with Alternative Feed Proteins and Bioactive Nutrients for Sustainable Egg Production and Global Food and Nutrition Security / Keith Jiajie Ou
- 발행사항
- [Sl] : Cornell University, 2025
- 형태사항
- 1 electronic resource (202 pages)
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
- 주기사항
- Advisors: Lei, Xingen Committee members: Danko, Charles; O'Brien, Kimberly; Duan, Jingyue.
- 학위논문주기
- - Ph.D. : Cornell University, 2025.
- 초록/해제
- 요약With the continuous growth of the global population and the corresponding increase in demand for animal-sourced foods, achieving sustainable production has become an urgent priority for the egg industry. Alternative feed proteins present promising potential to replace soybean meal, but further research is required to elucidate the full effects on animal health and egg production. Rich in bioactive nutrients such as omega-3 polyunsaturated fatty acids (n-3 PUFA), these ingredients also offer opportunities for egg biofortification with health-promoting nutrients. Therefore, the objectives of this research were to investigate the effects of innovative alternative protein feeding strategies in laying hen diets on animal health, egg production, and nutrient enrichment in eggs, as well as to evaluate the safety and metabolic impacts of consuming such biofortified eggs in laboratory mice.A series of laying hen experiments were conducted to examine the effects of dietary inclusions of hempseed meal alone or in combination with microalgae Nannochloropsis oceanica and black soldier fly larvae. The 10% hempseed meal supplementation, rich in omega-3 alpha-linolenic acid, increased (P 0.001) the egg yolk docosahexaenoic acid (DHA) content by 1.0-fold to 57.8 mg/egg compared with the control. The additions of 1.0% microalgae and 2.5% or 5.0% black soldier fly larvae did not further enhance egg n-3 PUFA levels but modulated (P 0.05) manure ammonia production relative to the hempseed meal only diet. Another laying hen study was conducted using microalgal DHA oil to further improve egg DHA and n-3 PUFA biofortification. Triple enrichment of DHA (16 mg/g yolk), 25-hydroxyvitamin D3 [25(OH)D3] (160 ng/g yolk) and astaxanthin (5.5 µg/yolk) was achieved by feeding laying hens 0.8% the DHA-rich microalgal oil, 200 ppb 25(OH)D3 supplement, and 0.16 ppm microalgal astaxanthin. Supplementing 0.8% microalgal DHA oil resulted in 160 mg DHA/egg, while co-supplementation with microalgal astaxanthin led to a 13% reductio in DHA enrichment. In a follow-up mouse study, consumption of the DHA-enriched yolks at the recommended dose led to hepatic DHA accumulation (up to 61%; P 0.001) without affecting plasma triacylglycerol concentrations compared to the control. However, consuming enriched yolks at 5-fold the recommended dose did not result in significant hepatic DHA deposition across diets. Another mouse study was performed to evaluate the effects of biofortified eggs with substantial 25(OH)D3 enrichment comparing to synthetic supplement under both healthy and obesity-inducing high-fat diet (HFD) conditions. Consuming 700 IU 25(OH)D3 per kg of feed from biofortified yolks were safe, and mice consumed the HFD with biofortified yolk exhibited 33.4% higher (P 0.01) plasma 25(OH)D3 level and upregulated kidney Cyp24a1 (P 0.01) and Vdr (P 0.05) mRNA expression compared to their HFD counterparts receiving synthetic 25(OH)D3 supplement.With comparable production performance, alternative feed proteins such as hempseed meal, microalgae, and insect protein, demonstrated strong potential to replace soybean meal at up to 50% in supporting sustainable egg production while concurrently produce health-promoting biofortified eggs with up to 160 mg of DHA and 140 IU of 25(OH)D3 per egg for public health nutrition.
- 언어주기
- English
- 일반주제명
- Animal sciences
- 일반주제명
- Nutrition
- 일반주제명
- Molecular biology
- 키워드
- Laboratory mouse
- 키워드
- Laying hen
- 키워드
- Vitamin D
- 기타저자
- Cornell University Animal Science
- 기본자료저록
- Dissertations Abstracts International. 86-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■1001 ▼aOu, Keith Jiajie▼eauthor.▼0(orcid)0000-0002-5597-0333
■24510▼aInnovating Feeding Laying Hens with Alternative Feed Proteins and Bioactive Nutrients for Sustainable Egg Production and Global Food and Nutrition Security ▼cKeith Jiajie Ou
■260 ▼a[Sl]▼bCornell University▼c2025
■264 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a1 electronic resource (202 pages)
■336 ▼atext▼btxt▼2rdacontent
■337 ▼acomputer▼bc▼2rdamedia
■338 ▼aonline resource▼bcr▼2rdacarrier
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-12, Section: B.
■500 ▼aAdvisors: Lei, Xingen Committee members: Danko, Charles; O'Brien, Kimberly; Duan, Jingyue.
■5021 ▼bPh.D.▼cCornell University▼d2025.
■520 ▼aWith the continuous growth of the global population and the corresponding increase in demand for animal-sourced foods, achieving sustainable production has become an urgent priority for the egg industry. Alternative feed proteins present promising potential to replace soybean meal, but further research is required to elucidate the full effects on animal health and egg production. Rich in bioactive nutrients such as omega-3 polyunsaturated fatty acids (n-3 PUFA), these ingredients also offer opportunities for egg biofortification with health-promoting nutrients. Therefore, the objectives of this research were to investigate the effects of innovative alternative protein feeding strategies in laying hen diets on animal health, egg production, and nutrient enrichment in eggs, as well as to evaluate the safety and metabolic impacts of consuming such biofortified eggs in laboratory mice.A series of laying hen experiments were conducted to examine the effects of dietary inclusions of hempseed meal alone or in combination with microalgae Nannochloropsis oceanica and black soldier fly larvae. The 10% hempseed meal supplementation, rich in omega-3 alpha-linolenic acid, increased (P 0.001) the egg yolk docosahexaenoic acid (DHA) content by 1.0-fold to 57.8 mg/egg compared with the control. The additions of 1.0% microalgae and 2.5% or 5.0% black soldier fly larvae did not further enhance egg n-3 PUFA levels but modulated (P 0.05) manure ammonia production relative to the hempseed meal only diet. Another laying hen study was conducted using microalgal DHA oil to further improve egg DHA and n-3 PUFA biofortification. Triple enrichment of DHA (16 mg/g yolk), 25-hydroxyvitamin D3 [25(OH)D3] (160 ng/g yolk) and astaxanthin (5.5 µg/yolk) was achieved by feeding laying hens 0.8% the DHA-rich microalgal oil, 200 ppb 25(OH)D3 supplement, and 0.16 ppm microalgal astaxanthin. Supplementing 0.8% microalgal DHA oil resulted in 160 mg DHA/egg, while co-supplementation with microalgal astaxanthin led to a 13% reductio in DHA enrichment. In a follow-up mouse study, consumption of the DHA-enriched yolks at the recommended dose led to hepatic DHA accumulation (up to 61%; P 0.001) without affecting plasma triacylglycerol concentrations compared to the control. However, consuming enriched yolks at 5-fold the recommended dose did not result in significant hepatic DHA deposition across diets. Another mouse study was performed to evaluate the effects of biofortified eggs with substantial 25(OH)D3 enrichment comparing to synthetic supplement under both healthy and obesity-inducing high-fat diet (HFD) conditions. Consuming 700 IU 25(OH)D3 per kg of feed from biofortified yolks were safe, and mice consumed the HFD with biofortified yolk exhibited 33.4% higher (P 0.01) plasma 25(OH)D3 level and upregulated kidney Cyp24a1 (P 0.01) and Vdr (P 0.05) mRNA expression compared to their HFD counterparts receiving synthetic 25(OH)D3 supplement.With comparable production performance, alternative feed proteins such as hempseed meal, microalgae, and insect protein, demonstrated strong potential to replace soybean meal at up to 50% in supporting sustainable egg production while concurrently produce health-promoting biofortified eggs with up to 160 mg of DHA and 140 IU of 25(OH)D3 per egg for public health nutrition.
■546 ▼aEnglish
■590 ▼aSchool code: 0058
■650 4▼aAnimal sciences
■650 4▼aNutrition
■650 4▼aMolecular biology
■653 ▼aAlternative feed protein
■653 ▼aEgg biofortification
■653 ▼aLaboratory mouse
■653 ▼aLaying hen
■653 ▼aOmega-3 polyunsaturated fatty acids
■653 ▼aVitamin D
■7102 ▼aCornell University▼bAnimal Science.▼edegree granting institution.
■7201 ▼aLei, Xingen▼edegree supervisor.
■7730 ▼tDissertations Abstracts International▼g86-12B.
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357201▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.


