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Effects of Lateralized Hippocampal Damage and Pituitary Gonadotrope Dysfunction in a Mouse Model of Temporal Lobe Epilepsy
Effects of Lateralized Hippocampal Damage and Pituitary Gonadotrope Dysfunction in a Mouse Model of Temporal Lobe Epilepsy
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105245
- ISBN
- 9798291572818
- DDC
- 616
- 서명/저자
- Effects of Lateralized Hippocampal Damage and Pituitary Gonadotrope Dysfunction in a Mouse Model of Temporal Lobe Epilepsy
- 발행사항
- [Sl] : University of Illinois at Urbana-Champaign, 2023
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2023
- 형태사항
- 172 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-03, Section: B.
- 주기사항
- Advisor: Christian-Hinman, Catherine.
- 학위논문주기
- Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2023.
- 초록/해제
- 요약Reproductive endocrine dysfunction is a common comorbidity of temporal lobe epilepsy (TLE). The specific mechanisms that link temporal lobe seizures to reproductive endocrine dysfunction remain unknown. However, clinical reports suggest that epilepsy-associated hypothalamic-pituitary-gonadal (HPG) axis dysfunction likely underlies the development of reproductive endocrine disorders. The intrahippocampal kainic acid (IHKA) mouse model of TLE also shows reproductive endocrine dysfunction as a majority of female IHKA treated animals display estrous cycle disruption. Furthermore, IHKA mice display alterations in the HPG axis as the activity of, and input to, hypothalamic gonadotropin-releasing hormone (GnRH) neurons is changed in IHKA females that develop estrous cycle elongation. However, further work in this model is needed to identify a specific mechanism that links the epilepsy-associated changes in hypothalamic circuitry to the development of reproductive endocrine dysfunction. The overall goal of this dissertation is to evaluate the progression and robustness of the reproductive endocrine dysfunction phenotype in female IHKA mice and to determine if epilepsy-associated hypothalamic changes in this model impact the pituitary. Chapter 2 investigates the differences in the presentation and progression of pituitary and gonadal phenotypic outcomes in female mice injected in the left vs. right dorsal hippocampus with KA. Chapter 3 continues to investigate the impacts of IHKA injection site by characterizing seizure burden differences in the left vs. right IHKA mice. Chapter 4 investigates the epilepsy-associated changes in pituitary release of gonadotropins and sensitivity to GnRH. The results from Chapter 2 and 3 indicate that the side of IHKA injection does not impact the development of comorbid estrous cycle disruption in females, but that KA injection site can impact pituitary gene expression and seizure burden in a sex- and estrous cycle-dependent manner. The results from Chapter 4 indicate that there are epilepsy-associated elevations in pituitary sensitivity to GnRH on certain estrous cycle stages in the absence of changes to the pattern of gonadotropin release. Overall, the data in this dissertation demonstrate that, within the IHKA model, lateralization of hippocampal damage can have implications on phenotypes present, and that temporal lobe seizures lead to altered pituitary function. This data demonstrates the usefulness of the IHKA model in the study of mechanisms linking temporal lobe seizures to altered reproductive endocrine function and vice versa.
- 일반주제명
- Neurosciences
- 일반주제명
- Physiology
- 일반주제명
- Health sciences
- 일반주제명
- Endocrinology
- 키워드
- Mouse model
- 기타저자
- University of Illinois at Urbana-Champaign Neuroscience Program
- 기본자료저록
- Dissertations Abstracts International. 87-03B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798291572818
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■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a616
■1001 ▼aCutia, Cathryn A.
■24510▼aEffects of Lateralized Hippocampal Damage and Pituitary Gonadotrope Dysfunction in a Mouse Model of Temporal Lobe Epilepsy
■260 ▼a[Sl]▼bUniversity of Illinois at Urbana-Champaign▼c2023
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2023
■300 ▼a172 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-03, Section: B.
■500 ▼aAdvisor: Christian-Hinman, Catherine.
■5021 ▼aThesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2023.
■520 ▼aReproductive endocrine dysfunction is a common comorbidity of temporal lobe epilepsy (TLE). The specific mechanisms that link temporal lobe seizures to reproductive endocrine dysfunction remain unknown. However, clinical reports suggest that epilepsy-associated hypothalamic-pituitary-gonadal (HPG) axis dysfunction likely underlies the development of reproductive endocrine disorders. The intrahippocampal kainic acid (IHKA) mouse model of TLE also shows reproductive endocrine dysfunction as a majority of female IHKA treated animals display estrous cycle disruption. Furthermore, IHKA mice display alterations in the HPG axis as the activity of, and input to, hypothalamic gonadotropin-releasing hormone (GnRH) neurons is changed in IHKA females that develop estrous cycle elongation. However, further work in this model is needed to identify a specific mechanism that links the epilepsy-associated changes in hypothalamic circuitry to the development of reproductive endocrine dysfunction. The overall goal of this dissertation is to evaluate the progression and robustness of the reproductive endocrine dysfunction phenotype in female IHKA mice and to determine if epilepsy-associated hypothalamic changes in this model impact the pituitary. Chapter 2 investigates the differences in the presentation and progression of pituitary and gonadal phenotypic outcomes in female mice injected in the left vs. right dorsal hippocampus with KA. Chapter 3 continues to investigate the impacts of IHKA injection site by characterizing seizure burden differences in the left vs. right IHKA mice. Chapter 4 investigates the epilepsy-associated changes in pituitary release of gonadotropins and sensitivity to GnRH. The results from Chapter 2 and 3 indicate that the side of IHKA injection does not impact the development of comorbid estrous cycle disruption in females, but that KA injection site can impact pituitary gene expression and seizure burden in a sex- and estrous cycle-dependent manner. The results from Chapter 4 indicate that there are epilepsy-associated elevations in pituitary sensitivity to GnRH on certain estrous cycle stages in the absence of changes to the pattern of gonadotropin release. Overall, the data in this dissertation demonstrate that, within the IHKA model, lateralization of hippocampal damage can have implications on phenotypes present, and that temporal lobe seizures lead to altered pituitary function. This data demonstrates the usefulness of the IHKA model in the study of mechanisms linking temporal lobe seizures to altered reproductive endocrine function and vice versa.
■590 ▼aSchool code: 0090.
■650 4▼aNeurosciences
■650 4▼aPhysiology
■650 4▼aHealth sciences
■650 4▼aEndocrinology
■653 ▼aReproductive endocrine dysfunction
■653 ▼aMouse model
■653 ▼aTemporal lobe epilepsy
■653 ▼aIntrahippocampal kainic acid
■653 ▼aGonadotropin-releasing hormone
■690 ▼a0317
■690 ▼a0566
■690 ▼a0409
■690 ▼a0719
■71020▼aUniversity of Illinois at Urbana-Champaign▼bNeuroscience Program.
■7730 ▼tDissertations Abstracts International▼g87-03B.
■790 ▼a0090
■791 ▼aPh.D.
■792 ▼a2023
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17359985▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
