서브메뉴
검색
Gene Expression, Regulation, and Implication in the Rat Models of Opioid Use Disorder
Gene Expression, Regulation, and Implication in the Rat Models of Opioid Use Disorder
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103539
- ISBN
- 9798290912530
- DDC
- 150
- 서명/저자
- Gene Expression, Regulation, and Implication in the Rat Models of Opioid Use Disorder
- 발행사항
- [Sl] : University of Minnesota, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 199 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-01, Section: B.
- 주기사항
- Advisor: Gewirtz, Jonathan C.;Tran, Phu V.
- 학위논문주기
- Thesis (Ph.D.)--University of Minnesota, 2025.
- 초록/해제
- 요약Substance use disorders (SUDs), including opioid use disorder (OUD), remain a major global health problem with substantial individual differences in vulnerability. Given the chronic nature of SUDs and the long-term impact of early-life experiences (e.g., childhood trauma) on addiction vulnerability in adulthood, changes in SUD vulnerability likely involve long-term epigenetic dysregulation. However, molecular mechanisms in the brain underlying individual differences in SUD vulnerability remain unclear. The goal of this dissertation was to evaluate how early-life experiences affect epigenetic regulation into adulthood using a prenatal-neonatal iron deficiency and choline supplementation rat model, and to characterize transcriptional and epigenetic signatures associated with morphine exposure and individual differences in OUD vulnerability in rat models capturing 3 distinct stages of human OUD. Study 1 showed that early-life ID and choline supplementation interacted to epigenetically regulate the expression of genes encoding JmjC-ARID domain-containing protein 1B and brain-derived neurotrophic factor, a potential biomarker for heroin use, in the rat hippocampus into adulthood. Study 2 showed that repeated exposure to the opioid morphine activated synaptogenesis and other neuroplasticity-related gene networks in the dorsomedial prefrontal cortex of rats. Study 3 showed that differential gene expression and chromatin accessibility in the medial prefrontal cortex were associated with individual differences in rat behavioral models of OUD. Overall, these studies emphasized the significant long-term effects of environmentally induced epigenetic dysregulation and extended the understanding of brain molecular mechanisms underlying individual differences in OUD vulnerability in rat models.
- 일반주제명
- Behavioral sciences
- 일반주제명
- Psychobiology
- 일반주제명
- Molecular biology
- 일반주제명
- Genetics
- 키워드
- Gene expression
- 기타저자
- University of Minnesota Psychology
- 기본자료저록
- Dissertations Abstracts International. 87-01B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
008260126s2025 us c eng d■001000017357634
■00520260202103539
■006m o d
■007cr#unu||||||||
■020 ▼a9798290912530
■035 ▼a(MiAaPQ)AAI32040791
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a150
■1001 ▼aLiu, Shirelle Xinyue.
■24510▼aGene Expression, Regulation, and Implication in the Rat Models of Opioid Use Disorder
■260 ▼a[Sl]▼bUniversity of Minnesota▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a199 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-01, Section: B.
■500 ▼aAdvisor: Gewirtz, Jonathan C.;Tran, Phu V.
■5021 ▼aThesis (Ph.D.)--University of Minnesota, 2025.
■520 ▼aSubstance use disorders (SUDs), including opioid use disorder (OUD), remain a major global health problem with substantial individual differences in vulnerability. Given the chronic nature of SUDs and the long-term impact of early-life experiences (e.g., childhood trauma) on addiction vulnerability in adulthood, changes in SUD vulnerability likely involve long-term epigenetic dysregulation. However, molecular mechanisms in the brain underlying individual differences in SUD vulnerability remain unclear. The goal of this dissertation was to evaluate how early-life experiences affect epigenetic regulation into adulthood using a prenatal-neonatal iron deficiency and choline supplementation rat model, and to characterize transcriptional and epigenetic signatures associated with morphine exposure and individual differences in OUD vulnerability in rat models capturing 3 distinct stages of human OUD. Study 1 showed that early-life ID and choline supplementation interacted to epigenetically regulate the expression of genes encoding JmjC-ARID domain-containing protein 1B and brain-derived neurotrophic factor, a potential biomarker for heroin use, in the rat hippocampus into adulthood. Study 2 showed that repeated exposure to the opioid morphine activated synaptogenesis and other neuroplasticity-related gene networks in the dorsomedial prefrontal cortex of rats. Study 3 showed that differential gene expression and chromatin accessibility in the medial prefrontal cortex were associated with individual differences in rat behavioral models of OUD. Overall, these studies emphasized the significant long-term effects of environmentally induced epigenetic dysregulation and extended the understanding of brain molecular mechanisms underlying individual differences in OUD vulnerability in rat models.
■590 ▼aSchool code: 0130.
■650 4▼aBehavioral sciences
■650 4▼aPsychobiology
■650 4▼aMolecular biology
■650 4▼aGenetics
■653 ▼aSubstance use disorders
■653 ▼aOpioid use disorder
■653 ▼aGene expression
■653 ▼aMolecular mechanisms
■690 ▼a0602
■690 ▼a0349
■690 ▼a0369
■690 ▼a0307
■71020▼aUniversity of Minnesota▼bPsychology.
■7730 ▼tDissertations Abstracts International▼g87-01B.
■790 ▼a0130
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357634▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
