서브메뉴
검색
Exploring the Roles of Auxin Transport and Stigmasterol in Plant Development
Exploring the Roles of Auxin Transport and Stigmasterol in Plant Development
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103144
- ISBN
- 9798286431359
- DDC
- 580
- 서명/저자
- Exploring the Roles of Auxin Transport and Stigmasterol in Plant Development
- 발행사항
- [Sl] : Iowa State University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 138 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-12, Section: A.
- 주기사항
- Advisor: Kelley, Dior R.
- 학위논문주기
- Thesis (Ph.D.)--Iowa State University, 2025.
- 초록/해제
- 요약Plants are essential for nearly every ecosystem, functioning as a primary food source, producing oxygen, regulating climate, filtering water, and shaping landscapes. Because most plants are unable to move freely, plants must adapt to their environment to survive. How plants grow and respond to their environment has been extensively studied, yet the complexity of plant biology leaves many mysteries unanswered. Hormones such as auxin and sterols are key influencers of plant development and environmental responses. This research focused on addressing key knowledge gaps in the hormone biology field using model plants Zea mays (maize) and Arabidopsis thaliana (thale cress). First, this study characterizes the roles of the evolutionarily conserved PIN-LIKES (PILS) auxin transporters, PILS2 and PILS6, in determining plant architecture traits in Arabidopsis and maize. Molecular and genetic analyses of the maize and Arabidopsis orthologs indicates that these two PILS orthologs have unique and non-redundant functions in organ formation. These findings highlight the importance of these proteins in maintaining auxin gradients within growing organs such as roots and shoots for proper organ size and formation. Additionally, this work investigated the role of one phytosterol, stigmasterol, in abiotic stress response. CYP710A8 is the final enzyme in stigmasterol biosynthesis in maize. An analysis of stigmasterol-deficient transcriptome shows significant changes in gene expression related to abiotic stress. Stigmasterol-deficient plants exhibit enhanced tolerance to salt stress, with altered levels of phytohormones, suggesting a compensatory mechanism that enhances resilience. This indicates that manipulating sterol biosynthetic pathways could be a strategy for developing stress-resilient crops without compromising growth under optimal conditions. These studies provide insights into the molecular mechanisms of plant growth and stress responses, emphasizing the roles of auxin transport and sitosterol to stigmasterol conversion. The research offers potential strategies for crop improvement, highlighting the significance of auxin homeostasis in organ size control and the potential of sterol engineering in enhancing stress tolerance. These findings contribute to our understanding of plant biology and the development of innovative approaches to improve agricultural productivity and sustainability.
- 일반주제명
- Plant sciences
- 일반주제명
- Agriculture
- 일반주제명
- Bioengineering
- 일반주제명
- Sustainability
- 키워드
- Auxin
- 키워드
- PIN-LIKES
- 키워드
- Stigmasterol
- 키워드
- Zea mays
- 키워드
- CYP710A8
- 기타저자
- Iowa State University Genetics Development and Cell Biology
- 기본자료저록
- Dissertations Abstracts International. 86-12A.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
008260126s2025 us c eng d■001000017357180
■00520260202103144
■006m o d
■007cr#unu||||||||
■020 ▼a9798286431359
■035 ▼a(MiAaPQ)AAI31994912
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a580
■1001 ▼aCowling, Craig Leroy.▼0(orcid)0000-0002-8081-0681
■24510▼aExploring the Roles of Auxin Transport and Stigmasterol in Plant Development
■260 ▼a[Sl]▼bIowa State University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a138 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-12, Section: A.
■500 ▼aAdvisor: Kelley, Dior R.
■5021 ▼aThesis (Ph.D.)--Iowa State University, 2025.
■520 ▼aPlants are essential for nearly every ecosystem, functioning as a primary food source, producing oxygen, regulating climate, filtering water, and shaping landscapes. Because most plants are unable to move freely, plants must adapt to their environment to survive. How plants grow and respond to their environment has been extensively studied, yet the complexity of plant biology leaves many mysteries unanswered. Hormones such as auxin and sterols are key influencers of plant development and environmental responses. This research focused on addressing key knowledge gaps in the hormone biology field using model plants Zea mays (maize) and Arabidopsis thaliana (thale cress). First, this study characterizes the roles of the evolutionarily conserved PIN-LIKES (PILS) auxin transporters, PILS2 and PILS6, in determining plant architecture traits in Arabidopsis and maize. Molecular and genetic analyses of the maize and Arabidopsis orthologs indicates that these two PILS orthologs have unique and non-redundant functions in organ formation. These findings highlight the importance of these proteins in maintaining auxin gradients within growing organs such as roots and shoots for proper organ size and formation. Additionally, this work investigated the role of one phytosterol, stigmasterol, in abiotic stress response. CYP710A8 is the final enzyme in stigmasterol biosynthesis in maize. An analysis of stigmasterol-deficient transcriptome shows significant changes in gene expression related to abiotic stress. Stigmasterol-deficient plants exhibit enhanced tolerance to salt stress, with altered levels of phytohormones, suggesting a compensatory mechanism that enhances resilience. This indicates that manipulating sterol biosynthetic pathways could be a strategy for developing stress-resilient crops without compromising growth under optimal conditions. These studies provide insights into the molecular mechanisms of plant growth and stress responses, emphasizing the roles of auxin transport and sitosterol to stigmasterol conversion. The research offers potential strategies for crop improvement, highlighting the significance of auxin homeostasis in organ size control and the potential of sterol engineering in enhancing stress tolerance. These findings contribute to our understanding of plant biology and the development of innovative approaches to improve agricultural productivity and sustainability.
■590 ▼aSchool code: 0097.
■650 4▼aPlant sciences
■650 4▼aAgriculture
■650 4▼aBioengineering
■650 4▼aSustainability
■653 ▼aAuxin
■653 ▼aPIN-LIKES
■653 ▼aStigmasterol
■653 ▼aZea mays
■653 ▼aCYP710A8
■690 ▼a0479
■690 ▼a0202
■690 ▼a0640
■690 ▼a0473
■71020▼aIowa State University▼bGenetics, Development and Cell Biology.
■7730 ▼tDissertations Abstracts International▼g86-12A.
■790 ▼a0097
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357180▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
