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Chemosensory Mechanisms That Shape Insect Behavior: Insights From Drosophila Into Molecular Targets for Vector Control
Chemosensory Mechanisms That Shape Insect Behavior: Insights From Drosophila Into Molecular Targets for Vector Control
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105146
- ISBN
- 9798297683730
- DDC
- 620.5
- 서명/저자
- Chemosensory Mechanisms That Shape Insect Behavior: Insights From Drosophila Into Molecular Targets for Vector Control
- 발행사항
- [Sl] : University of California, Santa Barbara, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 163 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-04, Section: B.
- 주기사항
- Advisor: Montell, Craig.
- 학위논문주기
- Thesis (Ph.D.)--University of California, Santa Barbara, 2025.
- 초록/해제
- 요약Mosquito bites transmit pathogens that cause significant morbidity and mortality globally. Upon landing, disease vectors like Aedes aegypti use contact chemosensation (taste) to decide whether to bite and thereby transmit disease. Although contact repellents (e.g., DEET) can deter feeding, their potency and duration remain limited, revealing gaps in our understanding of mosquito taste reception pathways and subsequent chemosensory adaptation. Leveraging the genetic and physiological toolkit of Drosophila melanogaster, we identify two conserved peripheral modules that shape taste-driven decisions and translate these insights to Aedes. First, we show that the TRP channel Painless (Pain) is required for fatty acid (FA)-evoked activity and behavior. FAs often suppress biting more effectively than DEET and present an opportunity to identify new targets for repellents. Yet, the receptor and cellular mechanisms that govern FA taste and gustatory adaptation in mosquitoes remain poorly defined. In Drosophila, Pain supports attraction to low FAs and aversion to high FAs via distinct gustatory receptor neuron classes. In Aedes, the ortholog Pain1 is necessary for FA-mediated aversion during both blood and nectar feeding and FA-evoked labellar spiking, while olfactory FA attraction remains intact. Heterologous assays indicate Pain is not directly gated by FAs at tested concentrations, consistent with indirect activation (i.e., GPCR-Gq/PLC signaling) or heteromeric TRP mechanisms. Second, we demonstrate that the CD36-family member, sensory neuron membrane protein 2 (snmp2), is expressed in bitter GRNs and accelerates response termination towards bitter compounds. Loss of snmp2 prolongs bitter-neuron firing and inhibition of appetitive pathways. Together, these results reveal arthropod-specific, targetable nodes that tune valence (Pain) and kinetics (SNMP2) of gustation, informing strategies for next-generation repellents with improved potency and longevity.
- 일반주제명
- Nanoscience
- 일반주제명
- Biology
- 일반주제명
- Molecular biology
- 키워드
- Aedes aegypti
- 키워드
- Fly behavior
- 키워드
- Insect behavior
- 기타저자
- University of California, Santa Barbara Dynamical Neuroscience
- 기본자료저록
- Dissertations Abstracts International. 87-04B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520260202105146
■006m o d
■007cr#unu||||||||
■020 ▼a9798297683730
■035 ▼a(MiAaPQ)AAI32241215
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a620.5
■1001 ▼aBontempo, Angela Elisa.
■24510▼aChemosensory Mechanisms That Shape Insect Behavior: Insights From Drosophila Into Molecular Targets for Vector Control
■260 ▼a[Sl]▼bUniversity of California, Santa Barbara▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a163 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-04, Section: B.
■500 ▼aAdvisor: Montell, Craig.
■5021 ▼aThesis (Ph.D.)--University of California, Santa Barbara, 2025.
■520 ▼aMosquito bites transmit pathogens that cause significant morbidity and mortality globally. Upon landing, disease vectors like Aedes aegypti use contact chemosensation (taste) to decide whether to bite and thereby transmit disease. Although contact repellents (e.g., DEET) can deter feeding, their potency and duration remain limited, revealing gaps in our understanding of mosquito taste reception pathways and subsequent chemosensory adaptation. Leveraging the genetic and physiological toolkit of Drosophila melanogaster, we identify two conserved peripheral modules that shape taste-driven decisions and translate these insights to Aedes. First, we show that the TRP channel Painless (Pain) is required for fatty acid (FA)-evoked activity and behavior. FAs often suppress biting more effectively than DEET and present an opportunity to identify new targets for repellents. Yet, the receptor and cellular mechanisms that govern FA taste and gustatory adaptation in mosquitoes remain poorly defined. In Drosophila, Pain supports attraction to low FAs and aversion to high FAs via distinct gustatory receptor neuron classes. In Aedes, the ortholog Pain1 is necessary for FA-mediated aversion during both blood and nectar feeding and FA-evoked labellar spiking, while olfactory FA attraction remains intact. Heterologous assays indicate Pain is not directly gated by FAs at tested concentrations, consistent with indirect activation (i.e., GPCR-Gq/PLC signaling) or heteromeric TRP mechanisms. Second, we demonstrate that the CD36-family member, sensory neuron membrane protein 2 (snmp2), is expressed in bitter GRNs and accelerates response termination towards bitter compounds. Loss of snmp2 prolongs bitter-neuron firing and inhibition of appetitive pathways. Together, these results reveal arthropod-specific, targetable nodes that tune valence (Pain) and kinetics (SNMP2) of gustation, informing strategies for next-generation repellents with improved potency and longevity.
■590 ▼aSchool code: 0035.
■650 4▼aNanoscience
■650 4▼aBiology
■650 4▼aMolecular biology
■653 ▼aAedes aegypti
■653 ▼aDrosophila melanogaster
■653 ▼aFly behavior
■653 ▼aInsect behavior
■653 ▼aMosquito behavior
■653 ▼aSensory neurobiology
■690 ▼a0565
■690 ▼a0306
■690 ▼a0307
■71020▼aUniversity of California, Santa Barbara▼bDynamical Neuroscience.
■7730 ▼tDissertations Abstracts International▼g87-04B.
■790 ▼a0035
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17359613▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
