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Impact of Disease Management Practices in Floriculture Crop Production on Phytophthora Species, Soilborne Diseases and Detection of P. nicotianae in North Carolina
Impact of Disease Management Practices in Floriculture Crop Production on Phytophthora Species, Soilborne Diseases and Detection of P. nicotianae in North Carolina
Detailed Information
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105631
- ISBN
- 9798297622760
- DDC
- 635.9
- 서명/저자
- Impact of Disease Management Practices in Floriculture Crop Production on Phytophthora Species, Soilborne Diseases and Detection of P. nicotianae in North Carolina
- 발행사항
- [Sl] : North Carolina State University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 157 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-04, Section: B.
- 주기사항
- Advisor: Beagle Ristaino, Jean.
- 학위논문주기
- Thesis (Ph.D.)--North Carolina State University, 2025.
- 초록/해제
- 요약Floriculture crop production is economically important to North Carolina. The crops are produced primarily in greenhouses and includes bedding plants such as petunia and annual vinca and herbaceous perennial plants such as coneflower and verbena. However, growers report that soilborne pathogens cause some of the most important disease problems they face.Management practices that suppress or prevent soilborne diseases in floriculture crop production under greenhouse conditions are known, but the extent that these practices are employed by floriculture crop producers in North Carolina is unknown. Understanding current practices can help identify areas in need of intervention that, if remedied, could potentially lead to improved disease management. In Chapter 1, 32 greenhouses growers in North Carolina were surveyed using a 35-question, in-person questionnaire that included topics related to soilborne disease occurrence to understand practices growers currently use, identify practices associated with the incidence of soilborne diseases, and identify research or extension needs for floriculture growers to mitigate soilborne diseases under greenhouse conditions. Survey results revealed disease suppressive production practices, such as treating irrigation water and inspecting propagation material before transplant, were used by most growers and associated with no disease occurrence. However, the incidence of soilborne diseases on annual vinca and petunia were often greater when conducive practices were employed. Results highlight the need for continued research and extension efforts, particularly in integrated pest management techniques to reduce crop losses to soilborne diseases.Floriculture crop producers report that species of Phytophthora are among the most common soilborne pathogens affecting their crops. Species of Phytophthora are particularly suited to survive and proliferate under greenhouse conditions because of the presence of a wide variety of hosts present, a continually conducive environment (warm and humid with frequent irrigation events), and their ability to produce survival structures (e.g., oospores and chlamydospores) which allows them to survive in plant debris or potting media between crops. Given that the international trade of ornamental plants plays a role in spreading pathogens worldwide, it is important to understand the species of Phytophthora affecting floriculture crops in North Carolina and characterize these pathogens to identify potentially new threats to floricultural crops. In Chapter 2, I identified and characterized species of Phytophthora collected from 32 floriculture crop greenhouses in North Carolina. P. nicotianae was the dominant species recovered, but P. drechsleri, P. heterospora, and P. tropicalis were also found. This is the first time a hybrid species, P. pseudocryptogea, has been recovered in North Carolina. Analysis of microsatellite markers for P. nicotianae revealed that some genotypes persisted from year to year in the same greenhouse. Closely related genotypes were shared among greenhouses and may have been spread via propagation material.Detection of P. nicotianae in floriculture crops is important for growers to implement management practices early to minimize crop loss. However, detection of P. nicotianae may be affected if systemic fungicides had been recently applied to infected plants. Loop-mediated isothermal amplification (LAMP) is a field-ready assay that detects DNA of a specific organism. A primer pair (F-loop/B-loop) was added to a previously published LAMP assay specific to P. nicotianae to improve the reaction time. In Chapter 3, detection of P. nicotianae in leaves of inoculated annual vinca plants treated with or without three systemic fungicides (cyazofamid, mefenoxam, or oxathiapiprolin) was compared using three detection methods: LAMP, culturing on semi-selective medium, and Agdia ImmunoStrip®. None of the systemic fungicides inhibited detection of P. nicotianae by the LAMP assay, but detection by ImmunoStrips® or culturing ranged from 80 to 100% and 60 to 100%, respectively. The LAMP assay specific to P. nicotianae may help diagnosticians detect the pathogen rapidly when culturing or ImmunoStrips® fail.
- 일반주제명
- Greenhouses
- 일반주제명
- By products
- 일반주제명
- Plant diseases
- 일반주제명
- Agriculture
- 키워드
- Floriculture
- 기본자료저록
- Dissertations Abstracts International. 87-04B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798297622760
■035 ▼a(MiAaPQ)AAI32331656
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a635.9
■1001 ▼aMarie Meadows, Inga.
■24510▼aImpact of Disease Management Practices in Floriculture Crop Production on Phytophthora Species, Soilborne Diseases and Detection of P. nicotianae in North Carolina
■260 ▼a[Sl]▼bNorth Carolina State University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a157 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-04, Section: B.
■500 ▼aAdvisor: Beagle Ristaino, Jean.
■5021 ▼aThesis (Ph.D.)--North Carolina State University, 2025.
■520 ▼aFloriculture crop production is economically important to North Carolina. The crops are produced primarily in greenhouses and includes bedding plants such as petunia and annual vinca and herbaceous perennial plants such as coneflower and verbena. However, growers report that soilborne pathogens cause some of the most important disease problems they face.Management practices that suppress or prevent soilborne diseases in floriculture crop production under greenhouse conditions are known, but the extent that these practices are employed by floriculture crop producers in North Carolina is unknown. Understanding current practices can help identify areas in need of intervention that, if remedied, could potentially lead to improved disease management. In Chapter 1, 32 greenhouses growers in North Carolina were surveyed using a 35-question, in-person questionnaire that included topics related to soilborne disease occurrence to understand practices growers currently use, identify practices associated with the incidence of soilborne diseases, and identify research or extension needs for floriculture growers to mitigate soilborne diseases under greenhouse conditions. Survey results revealed disease suppressive production practices, such as treating irrigation water and inspecting propagation material before transplant, were used by most growers and associated with no disease occurrence. However, the incidence of soilborne diseases on annual vinca and petunia were often greater when conducive practices were employed. Results highlight the need for continued research and extension efforts, particularly in integrated pest management techniques to reduce crop losses to soilborne diseases.Floriculture crop producers report that species of Phytophthora are among the most common soilborne pathogens affecting their crops. Species of Phytophthora are particularly suited to survive and proliferate under greenhouse conditions because of the presence of a wide variety of hosts present, a continually conducive environment (warm and humid with frequent irrigation events), and their ability to produce survival structures (e.g., oospores and chlamydospores) which allows them to survive in plant debris or potting media between crops. Given that the international trade of ornamental plants plays a role in spreading pathogens worldwide, it is important to understand the species of Phytophthora affecting floriculture crops in North Carolina and characterize these pathogens to identify potentially new threats to floricultural crops. In Chapter 2, I identified and characterized species of Phytophthora collected from 32 floriculture crop greenhouses in North Carolina. P. nicotianae was the dominant species recovered, but P. drechsleri, P. heterospora, and P. tropicalis were also found. This is the first time a hybrid species, P. pseudocryptogea, has been recovered in North Carolina. Analysis of microsatellite markers for P. nicotianae revealed that some genotypes persisted from year to year in the same greenhouse. Closely related genotypes were shared among greenhouses and may have been spread via propagation material.Detection of P. nicotianae in floriculture crops is important for growers to implement management practices early to minimize crop loss. However, detection of P. nicotianae may be affected if systemic fungicides had been recently applied to infected plants. Loop-mediated isothermal amplification (LAMP) is a field-ready assay that detects DNA of a specific organism. A primer pair (F-loop/B-loop) was added to a previously published LAMP assay specific to P. nicotianae to improve the reaction time. In Chapter 3, detection of P. nicotianae in leaves of inoculated annual vinca plants treated with or without three systemic fungicides (cyazofamid, mefenoxam, or oxathiapiprolin) was compared using three detection methods: LAMP, culturing on semi-selective medium, and Agdia ImmunoStrip®. None of the systemic fungicides inhibited detection of P. nicotianae by the LAMP assay, but detection by ImmunoStrips® or culturing ranged from 80 to 100% and 60 to 100%, respectively. The LAMP assay specific to P. nicotianae may help diagnosticians detect the pathogen rapidly when culturing or ImmunoStrips® fail.
■590 ▼aSchool code: 0155.
■650 4▼aGreenhouses
■650 4▼aBy products
■650 4▼aPlant diseases
■650 4▼aAgriculture
■653 ▼aFloriculture
■653 ▼aDisease management
■690 ▼a0473
■71020▼aNorth Carolina State University.
■7730 ▼tDissertations Abstracts International▼g87-04B.
■790 ▼a0155
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17360870▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
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