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Genomic Tools for Sweetpotato Variety Development: Genomic Selection, Fusarium Wilt Resistance, and High-Throughput Phenotyping for Guava Root-Knot Nematode Resistance
Genomic Tools for Sweetpotato Variety Development: Genomic Selection, Fusarium Wilt Resistance, and High-Throughput Phenotyping for Guava Root-Knot Nematode Resistance
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105632
- ISBN
- 9798297633575
- DDC
- 635
- 서명/저자
- Genomic Tools for Sweetpotato Variety Development: Genomic Selection, Fusarium Wilt Resistance, and High-Throughput Phenotyping for Guava Root-Knot Nematode Resistance
- 발행사항
- [Sl] : North Carolina State University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 140 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-04, Section: B.
- 주기사항
- Advisor: Yencho, G. Craig.
- 학위논문주기
- Thesis (Ph.D.)--North Carolina State University, 2025.
- 초록/해제
- 요약Sweetpotato (Ipomoea batatas (L.) Lam.) (2n = 6x = 90) is among the most important vegetable crops. Breeding sweetpotato cultivars for increased yield and quality traits, as well as resistance to pests and diseases, has been the focus of breeding programs globally. Genomic tools to facilitate earlier breeding decisions, like marker-assisted and genomic selection, have lagged largely due to the complex genetics of sweetpotato. Resources now exist that have allowed breeders to implement these technologies, including the development of reference genomes and genomic tools specific to polyploid crops. Chapter 1 of this dissertation is a literature review describing these resources and tools in more detail, as well as the production and breeding of sweetpotato and some of the constraints to variety development.Chapter 2 describes strategies for high-throughput phenotyping resistance to Meloidogyne enterolobii, the guava root-knot nematode, a quarantined pest in the state of North Carolina. These approaches utilized machine learning to identify and count nematode eggs, information which can inform breeders as to the resistance level of a given sweetpotato genotype. Using convolutional neural networks and human-defined parameters, a hybrid machine learning model was able to detect eggs as well as human evaluators (M. enterolobii R2 = 0.985, M. incognita R2 = 0.992, M. javanica R2 = 0.983). Automated counting protocols have the potential to save hundreds of hours of labor while enhancing genetic gain for resistance for plant breeders.Chapter 3 details a QTL analysis focused on resistance to Fusarium oxysporum f.sp. batatas, commonly called Fusarium wilt disease. This was once the most important disease in US sweetpotato production, however, cultivar resistance has largely addressed this issue. In breeding for resistance to M. enterolobii, population-level resistance to Fusarium wilt disease may decline as some nematode-resistant lines lack Fusarium resistance. Over three trials, we bioassayed a 454-clone biparental mapping population, NCDM04-0001 x 'Covington', and found a single major locus on chromosome 10, herein named qIbFo-10.1, that explained 33.8% of variation for resistance to Fusarium wilt, suggesting resistance may be controlled by one or a few tightly-linked loci. This locus should be considered a priority target for marker-assisted breeding.Chapter 4 of this dissertation addresses quantitative traits, including yield, shape, and USDA grade by deploying genome-wide markers which can be used to predict the parental breeding value of a given individual for these traits. We utilized the DArTag genotyping platform and performed field trials on both a training and breeding population, each with 528 genotypes representing the NC State sweetpotato breeding program's diversity. We compared BLUPs generated using mixed models and compared pedigree, genomic, and hybrid relationship matrices for trait predictions. Predictive abilities for the genomic BLUP model were highest in the breeding population for storage root count (r = 0.195) and length to diameter ratio (r = 0.317), and highest in the training population for total yield (r = 0.363) and USDA No. 1 storage root packout (r = 0.408). This provides evidence that genomic selection can increase the rate of genetic gain for quantitative traits in sweetpotato for the first time.Chapter 5 summarizes my experiences in the breeding program and includes details about several projects that were outside the scope of this dissertation. This chapter also identifies future research targets, including the use of drones for high-throughput phenotyping and future applications of genomic selection.This research strives to increase the rate of genetic gain for necessary traits for the sweetpotato breeding community. With the availability of genomic and high-throughput phenotyping tools, sweetpotato breeders can improve traits at the population-level, ultimately increasing the likelihood of selecting the next major sweetpotato variety.
- 일반주제명
- Horticulture
- 키워드
- Sweetpotato
- 키워드
- Fusarium
- 기본자료저록
- Dissertations Abstracts International. 87-04B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■006m o d
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■020 ▼a9798297633575
■035 ▼a(MiAaPQ)AAI32331788
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a635
■1001 ▼aFraher, Simon Phillip.
■24510▼aGenomic Tools for Sweetpotato Variety Development: Genomic Selection, Fusarium Wilt Resistance, and High-Throughput Phenotyping for Guava Root-Knot Nematode Resistance
■260 ▼a[Sl]▼bNorth Carolina State University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a140 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-04, Section: B.
■500 ▼aAdvisor: Yencho, G. Craig.
■5021 ▼aThesis (Ph.D.)--North Carolina State University, 2025.
■520 ▼aSweetpotato (Ipomoea batatas (L.) Lam.) (2n = 6x = 90) is among the most important vegetable crops. Breeding sweetpotato cultivars for increased yield and quality traits, as well as resistance to pests and diseases, has been the focus of breeding programs globally. Genomic tools to facilitate earlier breeding decisions, like marker-assisted and genomic selection, have lagged largely due to the complex genetics of sweetpotato. Resources now exist that have allowed breeders to implement these technologies, including the development of reference genomes and genomic tools specific to polyploid crops. Chapter 1 of this dissertation is a literature review describing these resources and tools in more detail, as well as the production and breeding of sweetpotato and some of the constraints to variety development.Chapter 2 describes strategies for high-throughput phenotyping resistance to Meloidogyne enterolobii, the guava root-knot nematode, a quarantined pest in the state of North Carolina. These approaches utilized machine learning to identify and count nematode eggs, information which can inform breeders as to the resistance level of a given sweetpotato genotype. Using convolutional neural networks and human-defined parameters, a hybrid machine learning model was able to detect eggs as well as human evaluators (M. enterolobii R2 = 0.985, M. incognita R2 = 0.992, M. javanica R2 = 0.983). Automated counting protocols have the potential to save hundreds of hours of labor while enhancing genetic gain for resistance for plant breeders.Chapter 3 details a QTL analysis focused on resistance to Fusarium oxysporum f.sp. batatas, commonly called Fusarium wilt disease. This was once the most important disease in US sweetpotato production, however, cultivar resistance has largely addressed this issue. In breeding for resistance to M. enterolobii, population-level resistance to Fusarium wilt disease may decline as some nematode-resistant lines lack Fusarium resistance. Over three trials, we bioassayed a 454-clone biparental mapping population, NCDM04-0001 x 'Covington', and found a single major locus on chromosome 10, herein named qIbFo-10.1, that explained 33.8% of variation for resistance to Fusarium wilt, suggesting resistance may be controlled by one or a few tightly-linked loci. This locus should be considered a priority target for marker-assisted breeding.Chapter 4 of this dissertation addresses quantitative traits, including yield, shape, and USDA grade by deploying genome-wide markers which can be used to predict the parental breeding value of a given individual for these traits. We utilized the DArTag genotyping platform and performed field trials on both a training and breeding population, each with 528 genotypes representing the NC State sweetpotato breeding program's diversity. We compared BLUPs generated using mixed models and compared pedigree, genomic, and hybrid relationship matrices for trait predictions. Predictive abilities for the genomic BLUP model were highest in the breeding population for storage root count (r = 0.195) and length to diameter ratio (r = 0.317), and highest in the training population for total yield (r = 0.363) and USDA No. 1 storage root packout (r = 0.408). This provides evidence that genomic selection can increase the rate of genetic gain for quantitative traits in sweetpotato for the first time.Chapter 5 summarizes my experiences in the breeding program and includes details about several projects that were outside the scope of this dissertation. This chapter also identifies future research targets, including the use of drones for high-throughput phenotyping and future applications of genomic selection.This research strives to increase the rate of genetic gain for necessary traits for the sweetpotato breeding community. With the availability of genomic and high-throughput phenotyping tools, sweetpotato breeders can improve traits at the population-level, ultimately increasing the likelihood of selecting the next major sweetpotato variety.
■590 ▼aSchool code: 0155.
■650 4▼aHorticulture
■653 ▼aSweetpotato
■653 ▼aFusarium
■690 ▼a0471
■71020▼aNorth Carolina State University.
■7730 ▼tDissertations Abstracts International▼g87-04B.
■790 ▼a0155
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17360876▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.


