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Breathing Underwater: From Oxygen Sensing to Behavior in Larval Zebrafish
Breathing Underwater: From Oxygen Sensing to Behavior in Larval Zebrafish
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103546
- ISBN
- 9798280713192
- DDC
- 574
- 서명/저자
- Breathing Underwater: From Oxygen Sensing to Behavior in Larval Zebrafish
- 발행사항
- [Sl] : Harvard University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 71 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
- 주기사항
- Advisor: Engert, Florian.
- 학위논문주기
- Thesis (Ph.D.)--Harvard University, 2025.
- 초록/해제
- 요약Oxygen (O₂) is essential for nearly all animal life, serving as the final electron acceptor in the mitochondrial electron transport chain and enabling the efficient generation of ATP to meet the energetic demands of multicellular organisms. However, despite its abundance in the atmosphere, oxygen availability is often unpredictable in aquatic environments. Many organisms, particularly aquatic vertebrates, must contend with acute drops in environmental oxygen or chronic hypoxic conditions during development. Understanding how vertebrates sense, respond to, and survive fluctuations in oxygen levels remains an important area of biological inquiry. Larval zebrafish (Danio rerio), with their optical transparency, genetic tractability, and quantifiable behaviors, provide a powerful model to investigate the neural circuits and behavioral strategies underlying hypoxia adaptation.This dissertation explores how larval zebrafish behaviorally and physiologically respond to hypoxia, employing a multidisciplinary approach that integrates behavioral assays, in vivo calcium imaging, laser ablation techniques, and serial electron microscopy-based circuit reconstruction. The studies presented here reveal that acute hypoxia triggers a robust and stereotyped motor behavior characterized by rhythmic pectoral fin movements. These movements likely serve an adaptive role by enhancing water flow across respiratory surfaces, thereby facilitating oxygen uptake. Calcium imaging experiments suggested specific motor neurons whose activity correlates with hypoxia-induced fin movements. Laser ablation of suggested nerve bundles confirmed the neural pathways controlling this behavior.Beyond acute responses, this dissertation examines the developmental consequences of chronic hypoxia exposure. Larval zebrafish raised under sustained low oxygen conditions exhibited altered growth patterns, including slow growth along the anteroposterior axis and a delayed onset of swim bladder inflation. Behavioral assays assessing the optomotor response revealed that chronic hypoxia impairs sensorimotor coordination during critical stages of development, potentially affecting the larvae's ability to navigate and forage effectively. These findings suggest that oxygen availability during early development has lasting impacts on morphology and neural circuit function.Together, these studies illuminate the strategies by which vertebrates transform oxygen-sensing into coordinated motor responses and offer a foundation for further investigations into the strategies for counteracting hypoxia, the interplay between environmental stress and nervous system development, and potential translational relevance for understanding hypoxia-related challenges in human health.
- 일반주제명
- Biology
- 일반주제명
- Aquatic sciences
- 일반주제명
- Neurosciences
- 일반주제명
- Cellular biology
- 일반주제명
- Molecular biology
- 키워드
- Behavior
- 키워드
- Calcium imaging
- 키워드
- Hypoxia
- 키워드
- Zebrafish
- 기타저자
- Harvard University Biology Molecular and Cellular
- 기본자료저록
- Dissertations Abstracts International. 86-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■006m o d
■007cr#unu||||||||
■020 ▼a9798280713192
■035 ▼a(MiAaPQ)AAI32041311
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aFoianini, Stephan.▼0(orcid)0009-0005-1166-0332
■24510▼aBreathing Underwater: From Oxygen Sensing to Behavior in Larval Zebrafish
■260 ▼a[Sl]▼bHarvard University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a71 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-12, Section: B.
■500 ▼aAdvisor: Engert, Florian.
■5021 ▼aThesis (Ph.D.)--Harvard University, 2025.
■520 ▼aOxygen (O₂) is essential for nearly all animal life, serving as the final electron acceptor in the mitochondrial electron transport chain and enabling the efficient generation of ATP to meet the energetic demands of multicellular organisms. However, despite its abundance in the atmosphere, oxygen availability is often unpredictable in aquatic environments. Many organisms, particularly aquatic vertebrates, must contend with acute drops in environmental oxygen or chronic hypoxic conditions during development. Understanding how vertebrates sense, respond to, and survive fluctuations in oxygen levels remains an important area of biological inquiry. Larval zebrafish (Danio rerio), with their optical transparency, genetic tractability, and quantifiable behaviors, provide a powerful model to investigate the neural circuits and behavioral strategies underlying hypoxia adaptation.This dissertation explores how larval zebrafish behaviorally and physiologically respond to hypoxia, employing a multidisciplinary approach that integrates behavioral assays, in vivo calcium imaging, laser ablation techniques, and serial electron microscopy-based circuit reconstruction. The studies presented here reveal that acute hypoxia triggers a robust and stereotyped motor behavior characterized by rhythmic pectoral fin movements. These movements likely serve an adaptive role by enhancing water flow across respiratory surfaces, thereby facilitating oxygen uptake. Calcium imaging experiments suggested specific motor neurons whose activity correlates with hypoxia-induced fin movements. Laser ablation of suggested nerve bundles confirmed the neural pathways controlling this behavior.Beyond acute responses, this dissertation examines the developmental consequences of chronic hypoxia exposure. Larval zebrafish raised under sustained low oxygen conditions exhibited altered growth patterns, including slow growth along the anteroposterior axis and a delayed onset of swim bladder inflation. Behavioral assays assessing the optomotor response revealed that chronic hypoxia impairs sensorimotor coordination during critical stages of development, potentially affecting the larvae's ability to navigate and forage effectively. These findings suggest that oxygen availability during early development has lasting impacts on morphology and neural circuit function.Together, these studies illuminate the strategies by which vertebrates transform oxygen-sensing into coordinated motor responses and offer a foundation for further investigations into the strategies for counteracting hypoxia, the interplay between environmental stress and nervous system development, and potential translational relevance for understanding hypoxia-related challenges in human health.
■590 ▼aSchool code: 0084.
■650 4▼aBiology
■650 4▼aAquatic sciences
■650 4▼aNeurosciences
■650 4▼aCellular biology
■650 4▼aMolecular biology
■653 ▼aBehavior
■653 ▼aCalcium imaging
■653 ▼aElectron microscopy
■653 ▼aHypoxia
■653 ▼aZebrafish
■690 ▼a0306
■690 ▼a0317
■690 ▼a0792
■690 ▼a0379
■690 ▼a0307
■71020▼aHarvard University▼bBiology, Molecular and Cellular.
■7730 ▼tDissertations Abstracts International▼g86-12B.
■790 ▼a0084
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357683▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
