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Implementing Sequencing Analysis to Uncover Spoilage and Food Safety Concerns Linked to the Microbiota of Food Processing Environments and Onions
Implementing Sequencing Analysis to Uncover Spoilage and Food Safety Concerns Linked to the Microbiota of Food Processing Environments and Onions
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202105316
- ISBN
- 9798273309722
- DDC
- 641
- 저자명
- Feng, Jingzhang.
- 서명/저자
- Implementing Sequencing Analysis to Uncover Spoilage and Food Safety Concerns Linked to the Microbiota of Food Processing Environments and Onions
- 발행사항
- [Sl] : Cornell University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 241 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-07, Section: B.
- 주기사항
- Includes supplementary digital materials.
- 주기사항
- Advisor: Snyder, Abigail.
- 학위논문주기
- Thesis (Ph.D.)--Cornell University, 2025.
- 초록/해제
- 요약Food processing environments and fresh produce harbor spoilage and pathogenic microbes that can affect food quality and safety. Assessing the distribution of microbes on food processing environment surfaces and fresh produce is essential for identifying food safety and quality concerns and for developing strategies to address these concerns. Conventionally, microbial assessments were conducted using culture-based methods to detect pathogens and enumerate indicator organisms. Advances in sequencing technologies have introduced new approaches for assessing microbes in processing environments and food products. For instance, amplicon sequencing has been widely used to analyze microbial community structures in food processing environments. However, multiple challenges exist in applying amplicon sequencing to characterize microbial communities in food production environments. This collection of work aims to address these challenges.In Chapter 1, we addressed the challenge of determining the harborage sites of spoilage organisms and pathogens using unstructured metadata. We analyzed 1,498 free-text swab site descriptions to identify common issues and language structures. We identified five information facets represented in these swab site descriptions and observed that 99.6% of these descriptions depicted only a single information facet about the swab site. To address problems associated with free-text swab site descriptions, we developed a metadata schema to standardize the structure and term usage of swab site descriptions. This schema was integrated into the One Health Enteric Package available at NCBI. Insufficient cell collection from processing environment surfaces and low DNA extraction yields can also pose challenges for characterizing microbial communities. Hence, in Chapter 2, we assessed the impact of cell recovery and DNA extraction protocols on the relative abundance estimates of microorganisms on artificially inoculated surfaces. We found that existing protocols for cell recovery and DNA extraction distorted the relative abundance of microorganisms on surfaces, indicating a need to improve current methods to enhance accuracy. In Chapter 3, we collected environmental samples from dairy facilities and conducted analyses using both amplicon sequencing and culturing methods to study microbial communities in dairy processing environments. The goal of these analyses was to understand the distribution of hygiene indicator genera (i.e., Gram-negative, Enterobacteriaceae, Spore-former, and Gram-positive genera) in dairy processing environments. These distributions provided insights into strategies for applying amplicon sequencing and culturing method to determine microbial harborage. In Chapter 4, we further applied amplicon sequencingx to study the impact of plant pathogens on the growth dynamics of Salmonella in onions. We found that plant pathogens can cause onion tissue decay and support Salmonella growth.
- 일반주제명
- Food science
- 일반주제명
- Microbiology
- 일반주제명
- Genetics
- 키워드
- DNA
- 키워드
- Metadata
- 키워드
- Ontology
- 키워드
- Pathogen
- 키워드
- Spoilage
- 기타저자
- Cornell University Food Science and Technology
- 기본자료저록
- Dissertations Abstracts International. 87-07B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798273309722
■035 ▼a(MiAaPQ)AAI32286516
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a641
■1001 ▼aFeng, Jingzhang.
■24510▼aImplementing Sequencing Analysis to Uncover Spoilage and Food Safety Concerns Linked to the Microbiota of Food Processing Environments and Onions
■260 ▼a[Sl]▼bCornell University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a241 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-07, Section: B.
■500 ▼aIncludes supplementary digital materials.
■500 ▼aAdvisor: Snyder, Abigail.
■5021 ▼aThesis (Ph.D.)--Cornell University, 2025.
■520 ▼aFood processing environments and fresh produce harbor spoilage and pathogenic microbes that can affect food quality and safety. Assessing the distribution of microbes on food processing environment surfaces and fresh produce is essential for identifying food safety and quality concerns and for developing strategies to address these concerns. Conventionally, microbial assessments were conducted using culture-based methods to detect pathogens and enumerate indicator organisms. Advances in sequencing technologies have introduced new approaches for assessing microbes in processing environments and food products. For instance, amplicon sequencing has been widely used to analyze microbial community structures in food processing environments. However, multiple challenges exist in applying amplicon sequencing to characterize microbial communities in food production environments. This collection of work aims to address these challenges.In Chapter 1, we addressed the challenge of determining the harborage sites of spoilage organisms and pathogens using unstructured metadata. We analyzed 1,498 free-text swab site descriptions to identify common issues and language structures. We identified five information facets represented in these swab site descriptions and observed that 99.6% of these descriptions depicted only a single information facet about the swab site. To address problems associated with free-text swab site descriptions, we developed a metadata schema to standardize the structure and term usage of swab site descriptions. This schema was integrated into the One Health Enteric Package available at NCBI. Insufficient cell collection from processing environment surfaces and low DNA extraction yields can also pose challenges for characterizing microbial communities. Hence, in Chapter 2, we assessed the impact of cell recovery and DNA extraction protocols on the relative abundance estimates of microorganisms on artificially inoculated surfaces. We found that existing protocols for cell recovery and DNA extraction distorted the relative abundance of microorganisms on surfaces, indicating a need to improve current methods to enhance accuracy. In Chapter 3, we collected environmental samples from dairy facilities and conducted analyses using both amplicon sequencing and culturing methods to study microbial communities in dairy processing environments. The goal of these analyses was to understand the distribution of hygiene indicator genera (i.e., Gram-negative, Enterobacteriaceae, Spore-former, and Gram-positive genera) in dairy processing environments. These distributions provided insights into strategies for applying amplicon sequencing and culturing method to determine microbial harborage. In Chapter 4, we further applied amplicon sequencingx to study the impact of plant pathogens on the growth dynamics of Salmonella in onions. We found that plant pathogens can cause onion tissue decay and support Salmonella growth.
■590 ▼aSchool code: 0058.
■650 4▼aFood science
■650 4▼aMicrobiology
■650 4▼aGenetics
■653 ▼aAmplicon sequencing
■653 ▼aDNA
■653 ▼aMetadata
■653 ▼aOntology
■653 ▼aPathogen
■653 ▼aSpoilage
■690 ▼a0359
■690 ▼a0410
■690 ▼a0369
■71020▼aCornell University▼bFood Science and Technology.
■7730 ▼tDissertations Abstracts International▼g87-07B.
■790 ▼a0058
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17360179▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.


