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Characterizing Changes to Nuclear Permeability in Budding Yeast Meiosis
Characterizing Changes to Nuclear Permeability in Budding Yeast Meiosis
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103550
- ISBN
- 9798288866265
- DDC
- 574
- 서명/저자
- Characterizing Changes to Nuclear Permeability in Budding Yeast Meiosis
- 발행사항
- [Sl] : University of California, Berkeley, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 101 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-01, Section: B.
- 주기사항
- Advisor: Unal, Elcin;Upadhyayula, Srigokul.
- 학위논문주기
- Thesis (Ph.D.)--University of California, Berkeley, 2025.
- 초록/해제
- 요약In eukaryotic organisms, the nucleus is remodeled to accommodate the space required to segregate DNA. Remodeling strategies range from closed division, where the nuclear envelope remains intact, to open divisions, where the nuclear envelope is temporarily disassembled. Organisms can employ different nuclear division strategies in different cell types or life stages. The budding yeast Saccharomyces cerevisiae undergoes closed mitosis, but its nuclear division strategy in meiosis is less well characterized. Here we investigate changes to nuclear permeability in budding yeast meiosis and find that meiosis II undergoes a semi-closed division called virtual nuclear envelope breakdown (vNEBD). During vNEBD there is bidirectional compartment mixing between the cytoplasm and nucleus, including RanGAP entering the nucleus. The relocalization of RanGAP, however, is dispensable for intercompartmental mixing to occur. Notably, vNEBD occurs without detectable nuclear envelope breakdown or nucleoporin dispersal and can be uncoupled from known nuclear pore complex remodeling events. We further identified that vNEBD is tightly regulated in the meiotic program by conserved kinase Ime2 and PP1/Glc7 phosphatase regulator Gip1. The mechanism of intercompartmental mixing in vNEBD is currently unknown but excitingly deviates from all other currently characterized mechanisms of semi-closed division. vNEBD is conserved between S. cerevisiae and the distantly related fission yeast Schizosaccharomyces pombe during meiosis II, suggesting a conserved function in gametogenesis.
- 일반주제명
- Cellular biology
- 일반주제명
- Genetics
- 일반주제명
- Microbiology
- 키워드
- Yeast meiosis
- 키워드
- vNEBD
- 키워드
- Gametogenesis
- 기타저자
- University of California, Berkeley Molecular & Cell Biology
- 기본자료저록
- Dissertations Abstracts International. 87-01B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520260202103550
■006m o d
■007cr#unu||||||||
■020 ▼a9798288866265
■035 ▼a(MiAaPQ)AAI32041739
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aWalsh, Matty Elle.
■24510▼aCharacterizing Changes to Nuclear Permeability in Budding Yeast Meiosis
■260 ▼a[Sl]▼bUniversity of California, Berkeley▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a101 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-01, Section: B.
■500 ▼aAdvisor: Unal, Elcin;Upadhyayula, Srigokul.
■5021 ▼aThesis (Ph.D.)--University of California, Berkeley, 2025.
■520 ▼aIn eukaryotic organisms, the nucleus is remodeled to accommodate the space required to segregate DNA. Remodeling strategies range from closed division, where the nuclear envelope remains intact, to open divisions, where the nuclear envelope is temporarily disassembled. Organisms can employ different nuclear division strategies in different cell types or life stages. The budding yeast Saccharomyces cerevisiae undergoes closed mitosis, but its nuclear division strategy in meiosis is less well characterized. Here we investigate changes to nuclear permeability in budding yeast meiosis and find that meiosis II undergoes a semi-closed division called virtual nuclear envelope breakdown (vNEBD). During vNEBD there is bidirectional compartment mixing between the cytoplasm and nucleus, including RanGAP entering the nucleus. The relocalization of RanGAP, however, is dispensable for intercompartmental mixing to occur. Notably, vNEBD occurs without detectable nuclear envelope breakdown or nucleoporin dispersal and can be uncoupled from known nuclear pore complex remodeling events. We further identified that vNEBD is tightly regulated in the meiotic program by conserved kinase Ime2 and PP1/Glc7 phosphatase regulator Gip1. The mechanism of intercompartmental mixing in vNEBD is currently unknown but excitingly deviates from all other currently characterized mechanisms of semi-closed division. vNEBD is conserved between S. cerevisiae and the distantly related fission yeast Schizosaccharomyces pombe during meiosis II, suggesting a conserved function in gametogenesis.
■590 ▼aSchool code: 0028.
■650 4▼aCellular biology
■650 4▼aGenetics
■650 4▼aMicrobiology
■653 ▼aYeast meiosis
■653 ▼avNEBD
■653 ▼aGametogenesis
■690 ▼a0379
■690 ▼a0369
■690 ▼a0410
■71020▼aUniversity of California, Berkeley▼bMolecular & Cell Biology.
■7730 ▼tDissertations Abstracts International▼g87-01B.
■790 ▼a0028
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357712▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
