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Selective Elimination of Vibrio Pathogens via the Synergy of a Type Six Secretion System and a Natural Phenazine Antibiotic
Selective Elimination of Vibrio Pathogens via the Synergy of a Type Six Secretion System and a Natural Phenazine Antibiotic
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103100
- ISBN
- 9798280711754
- DDC
- 576
- 서명/저자
- Selective Elimination of Vibrio Pathogens via the Synergy of a Type Six Secretion System and a Natural Phenazine Antibiotic
- 발행사항
- [Sl] : Harvard University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 187 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
- 주기사항
- Includes supplementary digital materials.
- 주기사항
- Advisor: Mekalanos, John J.
- 학위논문주기
- Thesis (Ph.D.)--Harvard University, 2025.
- 초록/해제
- 요약Vibrio species are most often studied in the context of human disease; multiple Vibrio species, including Vibrio cholerae, are widespread mammalian pathogens. In this work, we describe the isolation of a vibriocidal strain of Aeromonas dhakensis, named A603. We determine that its vibriocidal activity comes from a synergy between A603's Type Six Secretion System and a synthesized phenazine antibiotic, AdPhen. Remarkably, non-Vibrio taxa resist this killing. We identify 3 systems in Vibrio strains that confer increased AdPhen resistance. However, A603's T6SS activity overcomes efflux-mediated AdPhen resistance in newly generated V. cholerae mutants, likely through membrane potential dissipation and disruption of essential cation gradients. A603 uses both AdPhen and its T6SS to protect the host from invading Vibrio pathogens, like AHPND strains of V. parahaemolyticus. A603 guards aquatic hosts from AHPND-related microbiome disruptions without significant host colonization or changes to native flora. A603's easy application, low colonization of hosts, and minimal alteration of microbiome composition or function makes it a promising new probiotic for prevention of vibriosis in aquatic hosts. While A603 itself cannot colonize mammalian hosts, the lessons learned from its activity can be leveraged to improve prophylaxis and treatment of Vibrio diseases, including cholera.
- 일반주제명
- Microbiology
- 일반주제명
- Genetics
- 일반주제명
- Molecular biology
- 키워드
- A603
- 키워드
- Aeromonas
- 키워드
- Cholera
- 키워드
- Microbiome
- 키워드
- Phenazine
- 키워드
- Vibrio
- 기타저자
- Harvard University Biological and Biomedical Sciences
- 기본자료저록
- Dissertations Abstracts International. 86-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520260202103100
■006m o d
■007cr#unu||||||||
■020 ▼a9798280711754
■035 ▼a(MiAaPQ)AAI31933918
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a576
■1001 ▼aBier, Sarah Beth.▼0(orcid)0000-0002-9594-472X
■24510▼aSelective Elimination of Vibrio Pathogens via the Synergy of a Type Six Secretion System and a Natural Phenazine Antibiotic
■260 ▼a[Sl]▼bHarvard University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a187 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-12, Section: B.
■500 ▼aIncludes supplementary digital materials.
■500 ▼aAdvisor: Mekalanos, John J.
■5021 ▼aThesis (Ph.D.)--Harvard University, 2025.
■520 ▼aVibrio species are most often studied in the context of human disease; multiple Vibrio species, including Vibrio cholerae, are widespread mammalian pathogens. In this work, we describe the isolation of a vibriocidal strain of Aeromonas dhakensis, named A603. We determine that its vibriocidal activity comes from a synergy between A603's Type Six Secretion System and a synthesized phenazine antibiotic, AdPhen. Remarkably, non-Vibrio taxa resist this killing. We identify 3 systems in Vibrio strains that confer increased AdPhen resistance. However, A603's T6SS activity overcomes efflux-mediated AdPhen resistance in newly generated V. cholerae mutants, likely through membrane potential dissipation and disruption of essential cation gradients. A603 uses both AdPhen and its T6SS to protect the host from invading Vibrio pathogens, like AHPND strains of V. parahaemolyticus. A603 guards aquatic hosts from AHPND-related microbiome disruptions without significant host colonization or changes to native flora. A603's easy application, low colonization of hosts, and minimal alteration of microbiome composition or function makes it a promising new probiotic for prevention of vibriosis in aquatic hosts. While A603 itself cannot colonize mammalian hosts, the lessons learned from its activity can be leveraged to improve prophylaxis and treatment of Vibrio diseases, including cholera.
■590 ▼aSchool code: 0084.
■650 4▼aMicrobiology
■650 4▼aGenetics
■650 4▼aMolecular biology
■653 ▼aA603
■653 ▼aAeromonas
■653 ▼aCholera
■653 ▼aMicrobiome
■653 ▼aPhenazine
■653 ▼aVibrio
■690 ▼a0410
■690 ▼a0369
■690 ▼a0307
■71020▼aHarvard University▼bBiological and Biomedical Sciences.
■7730 ▼tDissertations Abstracts International▼g86-12B.
■790 ▼a0084
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17356911▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
