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Characterization of Factors Affecting Hormone Signaling in the Human Mammary Gland With Single-Cell Atlases
Characterization of Factors Affecting Hormone Signaling in the Human Mammary Gland With Single-Cell Atlases
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103155
- ISBN
- 9798280729711
- DDC
- 610
- 서명/저자
- Characterization of Factors Affecting Hormone Signaling in the Human Mammary Gland With Single-Cell Atlases
- 발행사항
- [Sl] : University of California, San Francisco, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 158 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
- 주기사항
- Advisor: Gartner, Zev.
- 학위논문주기
- Thesis (Ph.D.)--University of California, San Francisco, 2025.
- 초록/해제
- 요약The mammary gland is a dynamic organ with complex paracrine signaling downstream of hormone signaling. A history of pregnancy is protective against breast cancer over a lifetime, but this effect is strongest for an early first full birth. I have created a human breast tissue bank with human donor samples from breast reduction surgeries to study the effect of pregnancy on the breast tissue. A single-cell transcriptomic dataset and matched single-nucleus ATAC dataset were generated to describe the changes in the cell state and cell-cell signaling due to parity. The hormone receptor-positive luminal cells play a central role in the response to hormone signaling, and the degree of response is correlated with age and parity. Increasing age decreases the response to hormones, and parity modifies this relationship to suppress hormone response at the younger age. Cell-cell communications between different cell types are also suppressed in the parous mammary gland. There is a significant signature of interferon response the transcriptional state and accessible chromatin sites of the parous hormone receptor- positive luminal cells, as well as a broad inflammatory signal. These findings suggest a potential interaction of interferon response and hormone signaling in the parous breast. Finally, I generated a single-cell transcriptomics dataset to study the effects of testosterone replacement therapy on the breast tissue of transgender men, and found that testosterone suppresses estrogen signaling in these individuals, and thus potentially provides a protective effect against breast cancer.
- 일반주제명
- Bioengineering
- 일반주제명
- Cellular biology
- 일반주제명
- Oncology
- 일반주제명
- Obstetrics
- 일반주제명
- Genetics
- 키워드
- Epigenomics
- 키워드
- Mammary gland
- 기타저자
- University of California, San Francisco Bioengineering
- 기본자료저록
- Dissertations Abstracts International. 86-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798280729711
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■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a610
■1001 ▼aPhong, Kiet Tuan.▼0(orcid)0000-0002-9273-4276
■24510▼aCharacterization of Factors Affecting Hormone Signaling in the Human Mammary Gland With Single-Cell Atlases
■260 ▼a[Sl]▼bUniversity of California, San Francisco▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a158 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-12, Section: B.
■500 ▼aAdvisor: Gartner, Zev.
■5021 ▼aThesis (Ph.D.)--University of California, San Francisco, 2025.
■520 ▼aThe mammary gland is a dynamic organ with complex paracrine signaling downstream of hormone signaling. A history of pregnancy is protective against breast cancer over a lifetime, but this effect is strongest for an early first full birth. I have created a human breast tissue bank with human donor samples from breast reduction surgeries to study the effect of pregnancy on the breast tissue. A single-cell transcriptomic dataset and matched single-nucleus ATAC dataset were generated to describe the changes in the cell state and cell-cell signaling due to parity. The hormone receptor-positive luminal cells play a central role in the response to hormone signaling, and the degree of response is correlated with age and parity. Increasing age decreases the response to hormones, and parity modifies this relationship to suppress hormone response at the younger age. Cell-cell communications between different cell types are also suppressed in the parous mammary gland. There is a significant signature of interferon response the transcriptional state and accessible chromatin sites of the parous hormone receptor- positive luminal cells, as well as a broad inflammatory signal. These findings suggest a potential interaction of interferon response and hormone signaling in the parous breast. Finally, I generated a single-cell transcriptomics dataset to study the effects of testosterone replacement therapy on the breast tissue of transgender men, and found that testosterone suppresses estrogen signaling in these individuals, and thus potentially provides a protective effect against breast cancer.
■590 ▼aSchool code: 0034.
■650 4▼aBioengineering
■650 4▼aCellular biology
■650 4▼aOncology
■650 4▼aObstetrics
■650 4▼aGenetics
■653 ▼aEpigenomics
■653 ▼aHormone signaling
■653 ▼aMammary gland
■653 ▼aSingle-cell transcriptomics dataset
■690 ▼a0202
■690 ▼a0379
■690 ▼a0992
■690 ▼a0369
■690 ▼a0380
■71020▼aUniversity of California, San Francisco▼bBioengineering.
■7730 ▼tDissertations Abstracts International▼g86-12B.
■790 ▼a0034
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357247▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
