서브메뉴
검색
Cell Types of Interoception and Their Response to Acute Injury
Cell Types of Interoception and Their Response to Acute Injury
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103554
- ISBN
- 9798280719712
- DDC
- 616
- 저자명
- Kaye, Judith A.
- 서명/저자
- Cell Types of Interoception and Their Response to Acute Injury
- 발행사항
- [Sl] : Harvard University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 116 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
- 주기사항
- Advisor: Liberles, Stephen D.
- 학위논문주기
- Thesis (Ph.D.)--Harvard University, 2025.
- 초록/해제
- 요약Interoception is the ability of an organism to sense and regulate its internal states. Just as external sensory organs transduce environmental cues of various modalities, specialized cells are responsible for monitoring internal conditions within the body. However, unlike our exteroceptive abilities, the sensory mechanisms and circuits that underlie interoception are not well characterized and remain less understood.Cells that comprise the sensory tissues important for interoception are highly specialized and express unique proteins that confer their ability to perform their specific function. Single-cell RNA (scRNA) sequencing provides a snapshot of the transcriptome of individual cells and provides a comprehensive and unbiased approach to interrogating molecular diversity that enables transduction and transmission of internal status. To gain a better understanding of the molecular diversity that permits interoception, I have worked on multiple projects atlasing interoceptive sites. The first site assayed mixed primary and higher-order sensory neurons of the Area Postrema, an anatomically privileged brain region that is responsible for conveying the sense of visceral malaise. The second site includes enteroendocrine cells (EECs) - primary sensory cells of the gut responsible for communicating nutrient status in the digestive tract. Collaborative work on these projects highlights the advantage single-cell transcriptomics offers to the facilitation of scientific discovery.One major body-to-brain connection that has benefited from cell-type analysis in recent years is the vagus nerve. In the mouse, the vagus nerve is fused with the glossopharyngeal nerve. Afferents of these cranial nerves (CN IX and X) serve a wide array of physiological functions, helping establish tonic control of breathing, nutrient intake, blood pressure maintenance, and more. The cell bodies of CN IX and X reside in the nodose, petrosal, and jugular ganglia (NPJg) and are diverse in their transcriptomic landscapes. However, mapping the transcriptionally defined identity of a neuron to its physiological role has proven difficult. My thesis work combines the anatomy and physiology of interoception with single-cell transcriptomics, focusing on adding more biological context to NPJg scRNA data. Using single-nucleus RNA sequencing, we provide the first detailed molecular characterization of the response to acute axotomy in the NPJg. We observe that a common transcriptional program is activated in response to injury. We further investigate vagal subtypes based on transcriptomic profiles and their branch contributions, offering new insights into the relationship between molecular identity and the complex branching anatomy of the vagus and glossopharyngeal nerves.
- 일반주제명
- Neurosciences
- 일반주제명
- Biology
- 일반주제명
- Physiology
- 일반주제명
- Cellular biology
- 키워드
- Interoception
- 키워드
- Nerve injury
- 키워드
- Vagus nerve
- 기타저자
- Harvard University Biological and Biomedical Sciences
- 기본자료저록
- Dissertations Abstracts International. 86-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
008260126s2025 us c eng d■001000017357741
■00520260202103554
■006m o d
■007cr#unu||||||||
■020 ▼a9798280719712
■035 ▼a(MiAaPQ)AAI32041959
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a616
■1001 ▼aKaye, Judith A.▼0(orcid)0000-0002-3332-3020
■24510▼aCell Types of Interoception and Their Response to Acute Injury
■260 ▼a[Sl]▼bHarvard University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a116 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-12, Section: B.
■500 ▼aAdvisor: Liberles, Stephen D.
■5021 ▼aThesis (Ph.D.)--Harvard University, 2025.
■520 ▼aInteroception is the ability of an organism to sense and regulate its internal states. Just as external sensory organs transduce environmental cues of various modalities, specialized cells are responsible for monitoring internal conditions within the body. However, unlike our exteroceptive abilities, the sensory mechanisms and circuits that underlie interoception are not well characterized and remain less understood.Cells that comprise the sensory tissues important for interoception are highly specialized and express unique proteins that confer their ability to perform their specific function. Single-cell RNA (scRNA) sequencing provides a snapshot of the transcriptome of individual cells and provides a comprehensive and unbiased approach to interrogating molecular diversity that enables transduction and transmission of internal status. To gain a better understanding of the molecular diversity that permits interoception, I have worked on multiple projects atlasing interoceptive sites. The first site assayed mixed primary and higher-order sensory neurons of the Area Postrema, an anatomically privileged brain region that is responsible for conveying the sense of visceral malaise. The second site includes enteroendocrine cells (EECs) - primary sensory cells of the gut responsible for communicating nutrient status in the digestive tract. Collaborative work on these projects highlights the advantage single-cell transcriptomics offers to the facilitation of scientific discovery.One major body-to-brain connection that has benefited from cell-type analysis in recent years is the vagus nerve. In the mouse, the vagus nerve is fused with the glossopharyngeal nerve. Afferents of these cranial nerves (CN IX and X) serve a wide array of physiological functions, helping establish tonic control of breathing, nutrient intake, blood pressure maintenance, and more. The cell bodies of CN IX and X reside in the nodose, petrosal, and jugular ganglia (NPJg) and are diverse in their transcriptomic landscapes. However, mapping the transcriptionally defined identity of a neuron to its physiological role has proven difficult. My thesis work combines the anatomy and physiology of interoception with single-cell transcriptomics, focusing on adding more biological context to NPJg scRNA data. Using single-nucleus RNA sequencing, we provide the first detailed molecular characterization of the response to acute axotomy in the NPJg. We observe that a common transcriptional program is activated in response to injury. We further investigate vagal subtypes based on transcriptomic profiles and their branch contributions, offering new insights into the relationship between molecular identity and the complex branching anatomy of the vagus and glossopharyngeal nerves.
■590 ▼aSchool code: 0084.
■650 4▼aNeurosciences
■650 4▼aBiology
■650 4▼aPhysiology
■650 4▼aCellular biology
■653 ▼aInternal sensation
■653 ▼aInteroception
■653 ▼aNerve injury
■653 ▼aPeripheral nervous system
■653 ▼aSingle-cell sequencing
■653 ▼aVagus nerve
■690 ▼a0317
■690 ▼a0306
■690 ▼a0719
■690 ▼a0379
■71020▼aHarvard University▼bBiological and Biomedical Sciences.
■7730 ▼tDissertations Abstracts International▼g86-12B.
■790 ▼a0084
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357741▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
