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Computational Hyperspectral Microscopy for Bioimaging
Computational Hyperspectral Microscopy for Bioimaging
Computational Hyperspectral Microscopy for Bioimaging

Detailed Information

자료유형  
 학위논문 서양
최종처리일시  
20260202103555
ISBN  
9798288864551
DDC  
535
저자명  
Aggarwal, Neerja.
서명/저자  
Computational Hyperspectral Microscopy for Bioimaging
발행사항  
[Sl] : University of California, Berkeley, 2025
발행사항  
Ann Arbor : ProQuest Dissertations & Theses, 2025
형태사항  
91 p
주기사항  
Source: Dissertations Abstracts International, Volume: 87-01, Section: B.
주기사항  
Advisor: Waller, Laura.
학위논문주기  
Thesis (Ph.D.)--University of California, Berkeley, 2025.
초록/해제  
요약Hyperspectral imaging involves detecting the spectrum (intensity vs wavelength) of light emitted at each point in space. It has applications in biology such as fluorescence imaging of live cells and interferometry to see inside tissues. However, traditional hyperspectral systems often have to scan through this three-dimensional spatial-spectral datacube (x, y, λ) due to a 2D sensor, resulting in long acquisition times and large setups. Snapshot imaging fits the entire 3D datacube onto a 2D sensor at once but sacrifices resolution. Computational imaging involves the codesign of both optics and algorithms together to beat traditional tradeoffs. In this work, we present three imaging systems for various bioimaging applications that benefit from computational imaging to improve spectral imaging performance.In the first application, we redesigned a traditional spectrometer using a diffuser instead of a grating to diffract light. The resulting speckle pattern was captured using an image sensor and inverted to solve for the spectrum. This compact spectrometer was developed for optical coherence tomography, an interferometry technique for imaging eyes.In the second project for fluorescence microscopy, we used a diffuser to multiplex light onto a spectral filter array on an image sensor. We used compressed sensing to solve for more voxels in the hyperspectral data cube than pixels on the sensor. We developed a compact attachment for a traditional benchtop microscopy that enables live imaging on biological samples and demonstrate high fidelity reconstructions in experiment.In the final project, we adapted a Fourier ptychography system for spectral imaging using a filter array. Fourier ptychography uses angled illumination to scan through the spatial Fourier plane and build up a higher resolution image. By placing the filter array in the Fourier plane, we can scanned the object's spatial frequencies through each spectral filter to build up a high resolution spatio-spectral datacube. We investigated this idea via simulation and proposed an experimental setup that could be used for digital pathology.
일반주제명  
Optics
일반주제명  
Electrical engineering
일반주제명  
Computer science
일반주제명  
Medical imaging
키워드  
Bioimaging
키워드  
Computational imaging
키워드  
Hyperspectral data
키워드  
Microscopy
기타저자  
University of California, Berkeley Electrical Engineering & Computer Sciences
기본자료저록  
Dissertations Abstracts International. 87-01B.
전자적 위치 및 접속  
로그인 후 원문을 볼 수 있습니다.

MARC

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■035    ▼a(MiAaPQ)AAI32042001
■040    ▼aMiAaPQ▼cMiAaPQ
■0820  ▼a535
■1001  ▼aAggarwal,  Neerja.
■24510▼aComputational  Hyperspectral  Microscopy  for  Bioimaging
■260    ▼a[Sl]▼bUniversity  of  California,  Berkeley▼c2025
■260  1▼aAnn  Arbor▼bProQuest  Dissertations  &  Theses▼c2025
■300    ▼a91  p
■500    ▼aSource:  Dissertations  Abstracts  International,  Volume:  87-01,  Section:  B.
■500    ▼aAdvisor:  Waller,  Laura.
■5021  ▼aThesis  (Ph.D.)--University  of  California,  Berkeley,  2025.
■520    ▼aHyperspectral  imaging  involves  detecting  the  spectrum  (intensity  vs  wavelength)  of  light  emitted  at  each  point  in  space.  It  has  applications  in  biology  such  as  fluorescence  imaging  of  live  cells  and  interferometry  to  see  inside  tissues.  However,  traditional  hyperspectral  systems  often  have  to  scan  through  this  three-dimensional  spatial-spectral  datacube  (x,  y,  λ)  due  to  a  2D  sensor,  resulting  in  long  acquisition  times  and  large  setups.  Snapshot  imaging  fits  the  entire  3D  datacube  onto  a  2D  sensor  at  once  but  sacrifices  resolution.  Computational  imaging  involves  the  codesign  of  both  optics  and  algorithms  together  to  beat  traditional  tradeoffs.  In  this  work,  we  present  three  imaging  systems  for  various  bioimaging  applications  that  benefit  from  computational  imaging  to  improve  spectral  imaging  performance.In  the  first  application,  we  redesigned  a  traditional  spectrometer  using  a  diffuser  instead  of  a  grating  to  diffract  light.  The  resulting  speckle  pattern  was  captured  using  an  image  sensor  and  inverted  to  solve  for  the  spectrum.  This  compact  spectrometer  was  developed  for  optical  coherence  tomography,  an  interferometry  technique  for  imaging  eyes.In  the  second  project  for  fluorescence  microscopy,  we  used  a  diffuser  to  multiplex  light  onto  a  spectral  filter  array  on  an  image  sensor.  We  used  compressed  sensing  to  solve  for  more  voxels  in  the  hyperspectral  data  cube  than  pixels  on  the  sensor.  We  developed  a  compact  attachment  for  a  traditional  benchtop  microscopy  that  enables  live  imaging  on  biological  samples  and  demonstrate  high  fidelity  reconstructions  in  experiment.In  the  final  project,  we  adapted  a  Fourier  ptychography  system  for  spectral  imaging  using  a  filter  array.  Fourier  ptychography  uses  angled  illumination  to  scan  through  the  spatial  Fourier  plane  and  build  up  a  higher  resolution  image.  By  placing  the  filter  array  in  the  Fourier  plane,  we  can  scanned  the  object's  spatial  frequencies  through  each  spectral  filter  to  build  up  a  high  resolution  spatio-spectral  datacube.  We  investigated  this  idea  via  simulation  and  proposed  an  experimental  setup  that  could  be  used  for  digital  pathology.
■590    ▼aSchool  code:  0028.
■650  4▼aOptics
■650  4▼aElectrical  engineering
■650  4▼aComputer  science
■650  4▼aMedical  imaging
■653    ▼aBioimaging
■653    ▼aComputational  imaging
■653    ▼aHyperspectral  data
■653    ▼aMicroscopy
■690    ▼a0752
■690    ▼a0544
■690    ▼a0984
■690    ▼a0574
■71020▼aUniversity  of  California,  Berkeley▼bElectrical  Engineering  &  Computer  Sciences.
■7730  ▼tDissertations  Abstracts  International▼g87-01B.
■790    ▼a0028
■791    ▼aPh.D.
■792    ▼a2025
■793    ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357747▼nKERIS▼z이  자료의  원문은  한국교육학술정보원에서  제공합니다.

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