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Computational Hyperspectral Microscopy for Bioimaging
Computational Hyperspectral Microscopy for Bioimaging
Detailed Information
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103555
- ISBN
- 9798288864551
- DDC
- 535
- 서명/저자
- Computational Hyperspectral Microscopy for Bioimaging
- 발행사항
- [Sl] : University of California, Berkeley, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 91 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-01, Section: B.
- 주기사항
- Advisor: Waller, Laura.
- 학위논문주기
- Thesis (Ph.D.)--University of California, Berkeley, 2025.
- 초록/해제
- 요약Hyperspectral imaging involves detecting the spectrum (intensity vs wavelength) of light emitted at each point in space. It has applications in biology such as fluorescence imaging of live cells and interferometry to see inside tissues. However, traditional hyperspectral systems often have to scan through this three-dimensional spatial-spectral datacube (x, y, λ) due to a 2D sensor, resulting in long acquisition times and large setups. Snapshot imaging fits the entire 3D datacube onto a 2D sensor at once but sacrifices resolution. Computational imaging involves the codesign of both optics and algorithms together to beat traditional tradeoffs. In this work, we present three imaging systems for various bioimaging applications that benefit from computational imaging to improve spectral imaging performance.In the first application, we redesigned a traditional spectrometer using a diffuser instead of a grating to diffract light. The resulting speckle pattern was captured using an image sensor and inverted to solve for the spectrum. This compact spectrometer was developed for optical coherence tomography, an interferometry technique for imaging eyes.In the second project for fluorescence microscopy, we used a diffuser to multiplex light onto a spectral filter array on an image sensor. We used compressed sensing to solve for more voxels in the hyperspectral data cube than pixels on the sensor. We developed a compact attachment for a traditional benchtop microscopy that enables live imaging on biological samples and demonstrate high fidelity reconstructions in experiment.In the final project, we adapted a Fourier ptychography system for spectral imaging using a filter array. Fourier ptychography uses angled illumination to scan through the spatial Fourier plane and build up a higher resolution image. By placing the filter array in the Fourier plane, we can scanned the object's spatial frequencies through each spectral filter to build up a high resolution spatio-spectral datacube. We investigated this idea via simulation and proposed an experimental setup that could be used for digital pathology.
- 일반주제명
- Optics
- 일반주제명
- Electrical engineering
- 일반주제명
- Computer science
- 일반주제명
- Medical imaging
- 키워드
- Bioimaging
- 키워드
- Microscopy
- 기타저자
- University of California, Berkeley Electrical Engineering & Computer Sciences
- 기본자료저록
- Dissertations Abstracts International. 87-01B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
008260126s2025 us c eng d■001000017357747
■00520260202103555
■006m o d
■007cr#unu||||||||
■020 ▼a9798288864551
■035 ▼a(MiAaPQ)AAI32042001
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a535
■1001 ▼aAggarwal, Neerja.
■24510▼aComputational Hyperspectral Microscopy for Bioimaging
■260 ▼a[Sl]▼bUniversity of California, Berkeley▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a91 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-01, Section: B.
■500 ▼aAdvisor: Waller, Laura.
■5021 ▼aThesis (Ph.D.)--University of California, Berkeley, 2025.
■520 ▼aHyperspectral imaging involves detecting the spectrum (intensity vs wavelength) of light emitted at each point in space. It has applications in biology such as fluorescence imaging of live cells and interferometry to see inside tissues. However, traditional hyperspectral systems often have to scan through this three-dimensional spatial-spectral datacube (x, y, λ) due to a 2D sensor, resulting in long acquisition times and large setups. Snapshot imaging fits the entire 3D datacube onto a 2D sensor at once but sacrifices resolution. Computational imaging involves the codesign of both optics and algorithms together to beat traditional tradeoffs. In this work, we present three imaging systems for various bioimaging applications that benefit from computational imaging to improve spectral imaging performance.In the first application, we redesigned a traditional spectrometer using a diffuser instead of a grating to diffract light. The resulting speckle pattern was captured using an image sensor and inverted to solve for the spectrum. This compact spectrometer was developed for optical coherence tomography, an interferometry technique for imaging eyes.In the second project for fluorescence microscopy, we used a diffuser to multiplex light onto a spectral filter array on an image sensor. We used compressed sensing to solve for more voxels in the hyperspectral data cube than pixels on the sensor. We developed a compact attachment for a traditional benchtop microscopy that enables live imaging on biological samples and demonstrate high fidelity reconstructions in experiment.In the final project, we adapted a Fourier ptychography system for spectral imaging using a filter array. Fourier ptychography uses angled illumination to scan through the spatial Fourier plane and build up a higher resolution image. By placing the filter array in the Fourier plane, we can scanned the object's spatial frequencies through each spectral filter to build up a high resolution spatio-spectral datacube. We investigated this idea via simulation and proposed an experimental setup that could be used for digital pathology.
■590 ▼aSchool code: 0028.
■650 4▼aOptics
■650 4▼aElectrical engineering
■650 4▼aComputer science
■650 4▼aMedical imaging
■653 ▼aBioimaging
■653 ▼aComputational imaging
■653 ▼aHyperspectral data
■653 ▼aMicroscopy
■690 ▼a0752
■690 ▼a0544
■690 ▼a0984
■690 ▼a0574
■71020▼aUniversity of California, Berkeley▼bElectrical Engineering & Computer Sciences.
■7730 ▼tDissertations Abstracts International▼g87-01B.
■790 ▼a0028
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357747▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
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