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Bacillus subtilis Cell Wall Twisting upon Cleavage: Evidence for Stress and Strain
Bacillus subtilis Cell Wall Twisting upon Cleavage: Evidence for Stress and Strain
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103555
- ISBN
- 9798280712935
- DDC
- 574
- 서명/저자
- Bacillus subtilis Cell Wall Twisting upon Cleavage: Evidence for Stress and Strain
- 발행사항
- [Sl] : Harvard University, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 72 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
- 주기사항
- Includes supplementary digital materials.
- 주기사항
- Advisor: Garner, Ethan C.
- 학위논문주기
- Thesis (Ph.D.)--Harvard University, 2025.
- 초록/해제
- 요약Bacterial cells exhibit chiral twisting during elongation, a phenomenon previously observed in Bacillus subtilis macrofibers and more recently in Escherichia coli. While twisting has been linked to cell wall mechanics, the precise biophysical mechanisms underlying this behavior remain unclear. Here, we investigate how the balance between cell wall synthesis and hydrolase activity influences twisting in B. subtilis. We propose that torsional stress accumulates within the peptidoglycan network due to circumferentially oriented glycan insertion, which distributes stress on the wall from turgor and is released upon hydrolase-mediated cleavage. Supporting this model, we find that cells deficient in hydrolases twist significantly less and have morphological effects of buckling and coiling, suggesting that cleavage events relieve torsional strain. Increasing Rod complex activity by deleting ponA accelerates twisting, reinforcing the idea that stress accumulates from increased circumferential insertion as the wall expands. Notably, chiral rotation occurs concurrently with cell separation, further demonstrating the presence of stored torsional stress. Unlike E. coli, where MreB filament orientation correlates with twisting, B. subtilis twisting appears independent of MreB motion, suggesting an alternative mechanism rooted in peptidoglycan crosslinking and stress redistribution. Additionally, it is proposed that torsional stress originates from turgor pressure. Our findings establish a direct link between bacterial mechanobiology and cell wall remodeling, highlighting the role of hydrolases in modulating torsional forces during growth.
- 일반주제명
- Cellular biology
- 일반주제명
- Biophysics
- 일반주제명
- Microbiology
- 키워드
- Cell wall
- 키워드
- Chiral twisting
- 키워드
- Peptidoglycan
- 기타저자
- Harvard University Biology Molecular and Cellular
- 기본자료저록
- Dissertations Abstracts International. 86-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798280712935
■035 ▼a(MiAaPQ)AAI32042002
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aHenthorn, Daniel.▼0(orcid)0009-0008-6124-6756
■24510▼aBacillus subtilis Cell Wall Twisting upon Cleavage: Evidence for Stress and Strain
■260 ▼a[Sl]▼bHarvard University▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a72 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-12, Section: B.
■500 ▼aIncludes supplementary digital materials.
■500 ▼aAdvisor: Garner, Ethan C.
■5021 ▼aThesis (Ph.D.)--Harvard University, 2025.
■520 ▼aBacterial cells exhibit chiral twisting during elongation, a phenomenon previously observed in Bacillus subtilis macrofibers and more recently in Escherichia coli. While twisting has been linked to cell wall mechanics, the precise biophysical mechanisms underlying this behavior remain unclear. Here, we investigate how the balance between cell wall synthesis and hydrolase activity influences twisting in B. subtilis. We propose that torsional stress accumulates within the peptidoglycan network due to circumferentially oriented glycan insertion, which distributes stress on the wall from turgor and is released upon hydrolase-mediated cleavage. Supporting this model, we find that cells deficient in hydrolases twist significantly less and have morphological effects of buckling and coiling, suggesting that cleavage events relieve torsional strain. Increasing Rod complex activity by deleting ponA accelerates twisting, reinforcing the idea that stress accumulates from increased circumferential insertion as the wall expands. Notably, chiral rotation occurs concurrently with cell separation, further demonstrating the presence of stored torsional stress. Unlike E. coli, where MreB filament orientation correlates with twisting, B. subtilis twisting appears independent of MreB motion, suggesting an alternative mechanism rooted in peptidoglycan crosslinking and stress redistribution. Additionally, it is proposed that torsional stress originates from turgor pressure. Our findings establish a direct link between bacterial mechanobiology and cell wall remodeling, highlighting the role of hydrolases in modulating torsional forces during growth.
■590 ▼aSchool code: 0084.
■650 4▼aCellular biology
■650 4▼aBiophysics
■650 4▼aMicrobiology
■653 ▼aBacillus subtilis
■653 ▼aCell wall
■653 ▼aChiral twisting
■653 ▼aPeptidoglycan
■690 ▼a0379
■690 ▼a0786
■690 ▼a0410
■71020▼aHarvard University▼bBiology, Molecular and Cellular.
■7730 ▼tDissertations Abstracts International▼g86-12B.
■790 ▼a0084
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357748▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
