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Tool Development and Comparative Multi-Omics Studies of Zn, Cu, and Fe Homeostasis in an Emerging Reference Alga: Auxenochlorella. sp. (UTEX 250-A)
Tool Development and Comparative Multi-Omics Studies of Zn, Cu, and Fe Homeostasis in an Emerging Reference Alga: Auxenochlorella. sp. (UTEX 250-A)
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202103600
- ISBN
- 9798288865848
- DDC
- 574
- 서명/저자
- Tool Development and Comparative Multi-Omics Studies of Zn, Cu, and Fe Homeostasis in an Emerging Reference Alga: Auxenochlorella. sp. (UTEX 250-A)
- 발행사항
- [Sl] : University of California, Berkeley, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 322 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-01, Section: B.
- 주기사항
- Includes supplementary digital materials.
- 주기사항
- Advisor: Merchant, Sabeeha S.
- 학위논문주기
- Thesis (Ph.D.)--University of California, Berkeley, 2025.
- 초록/해제
- 요약Algae are of global and industrial importance, as they are major contributors to net primary productivity and hold promise for the sustainable production of energy, food, medicine, and other natural products. Auxenochlorella sp. strain UTEX 250-A, is a prime candidate for both industrial applications and scientific inquiry, as it exhibits rapid growth, high lipid content, trophic flexibility, and is amenable to genetic transformation and targeted reverse genetics approaches via homologous recombination. Leveraging UTEX 250-A as a platform for discovery and bioproduction requires a deeper understanding of its nutritional requirements and responses to nutrient limitation. The first part of this dissertation focuses on developing tools and protocols to probe the physiological and molecular characteristics of UTEX 250-A. In chapter two, I present protocols adapted from the well-studied reference alga Chlamydomonas reinhardtii, considering differences in cell morphology and behavior. In chapter three, I investigate the nutritional requirements of UTEX 250-A during mixotrophic growth in the presence of 2% glucose and light. I then introduce a custom-formulated medium that supports robust growth through the stationary phase and accounts for metabolic shifts that occur thereafter. In Chapter four, I take a combined transcriptomics and proteomics approach to map the molecular mechanisms involved in maintaining metal homeostasis during Zn, Cu, and Fe starvation. This dissertation lays the groundwork for future systems level analysis and targeted investigations in UTEX250-A.
- 일반주제명
- Molecular biology
- 일반주제명
- Medicine
- 일반주제명
- Materials science
- 일반주제명
- Microbiology
- 키워드
- Algae
- 키워드
- Auxenochlorella
- 키워드
- Copper
- 키워드
- Iron
- 키워드
- Zinc
- 기타저자
- University of California, Berkeley Molecular & Cell Biology
- 기본자료저록
- Dissertations Abstracts International. 87-01B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520260202103600
■006m o d
■007cr#unu||||||||
■020 ▼a9798288865848
■035 ▼a(MiAaPQ)AAI32042435
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aCamacho, Dimitrios Joseph.
■24510▼aTool Development and Comparative Multi-Omics Studies of Zn, Cu, and Fe Homeostasis in an Emerging Reference Alga: Auxenochlorella. sp. (UTEX 250-A)
■260 ▼a[Sl]▼bUniversity of California, Berkeley▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a322 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-01, Section: B.
■500 ▼aIncludes supplementary digital materials.
■500 ▼aAdvisor: Merchant, Sabeeha S.
■5021 ▼aThesis (Ph.D.)--University of California, Berkeley, 2025.
■520 ▼aAlgae are of global and industrial importance, as they are major contributors to net primary productivity and hold promise for the sustainable production of energy, food, medicine, and other natural products. Auxenochlorella sp. strain UTEX 250-A, is a prime candidate for both industrial applications and scientific inquiry, as it exhibits rapid growth, high lipid content, trophic flexibility, and is amenable to genetic transformation and targeted reverse genetics approaches via homologous recombination. Leveraging UTEX 250-A as a platform for discovery and bioproduction requires a deeper understanding of its nutritional requirements and responses to nutrient limitation. The first part of this dissertation focuses on developing tools and protocols to probe the physiological and molecular characteristics of UTEX 250-A. In chapter two, I present protocols adapted from the well-studied reference alga Chlamydomonas reinhardtii, considering differences in cell morphology and behavior. In chapter three, I investigate the nutritional requirements of UTEX 250-A during mixotrophic growth in the presence of 2% glucose and light. I then introduce a custom-formulated medium that supports robust growth through the stationary phase and accounts for metabolic shifts that occur thereafter. In Chapter four, I take a combined transcriptomics and proteomics approach to map the molecular mechanisms involved in maintaining metal homeostasis during Zn, Cu, and Fe starvation. This dissertation lays the groundwork for future systems level analysis and targeted investigations in UTEX250-A.
■590 ▼aSchool code: 0028.
■650 4▼aMolecular biology
■650 4▼aMedicine
■650 4▼aMaterials science
■650 4▼aMicrobiology
■653 ▼aAlgae
■653 ▼aAuxenochlorella
■653 ▼aCopper
■653 ▼aIron
■653 ▼aZinc
■653 ▼aChlamydomonas reinhardtii
■690 ▼a0307
■690 ▼a0794
■690 ▼a0564
■690 ▼a0410
■71020▼aUniversity of California, Berkeley▼bMolecular & Cell Biology.
■7730 ▼tDissertations Abstracts International▼g87-01B.
■790 ▼a0028
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17357790▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
