서브메뉴
검색
Diversity, Activity, and Adaptations of Phage Communities in Anoxic Hydrocarbon-Rich Marine Sediments
Diversity, Activity, and Adaptations of Phage Communities in Anoxic Hydrocarbon-Rich Marine Sediments
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20260202104751
- ISBN
- 9798290657035
- DDC
- 600
- 서명/저자
- Diversity, Activity, and Adaptations of Phage Communities in Anoxic Hydrocarbon-Rich Marine Sediments
- 발행사항
- [Sl] : California Institute of Technology, 2025
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2025
- 형태사항
- 169 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 87-01, Section: B.
- 주기사항
- Advisor: Orphan, Victoria.
- 학위논문주기
- Thesis (Ph.D.)--California Institute of Technology, 2025.
- 초록/해제
- 요약The viruses of the global ocean, especially those infecting prokaryotic taxa, are known to play an important role in maintaining the genetic and taxonomic diversity of their host communities and in the cycling of atmospheric carbon and key nutrients like nitrogen and iron. However, the vast majority of these conclusions are drawn from the surface ocean and upper water column, while the sediments, which constitute one of the largest biomes on earth, are understudied in comparison. Of special interest are areas on the ocean floor where methane and other hydrocarbons are produced and released by geological activity and oxidized by a consortium of archaea and bacteria. Using direct genomic sequencing of the viruses from a variety of simplified sediment-free enrichments of hydrocarbon oxidizers, I compare viral communities sampled from different locations and incubated under a range of temperatures to understand the role these parameters might play in shaping distribution and community structure. I then present the most comprehensive picture thus far of viral diversity and distribution from a methane cold seep and discuss whether the viral assemblages are influenced by the steep geochemical gradients that characterize seep sediments. From these datasets, I propose that viral communities in methane-oxidizing sediments are tailored specifically to the physical constraints of the sediment matrix rather than to the dominant members of the cellular community or to other physicochemical parameters such as temperature, sampling location, or depth below the seafloor. I then outline the development of two methods, stable-isotope probing coupled to nanoSIMS and biorthogonal non-canonical amino acid tagging, to work in heterogenous sediment virus samples rather than the liquid pure cultures on which they had previously relied. Implementation of these methods, which allow us to temporally constrain viral production and virus-influenced nutrient flow, resulted in the hypothesis that viral production likely responds to shifts in the major metabolic processes within an ecosystem and may influence cellular community composition.
- 일반주제명
- Infections
- 일반주제명
- Hydrocarbons
- 일반주제명
- Chemical reactions
- 일반주제명
- Methane
- 일반주제명
- Bacteria
- 일반주제명
- Genomes
- 일반주제명
- Energy
- 일반주제명
- Metabolism
- 일반주제명
- Ecosystems
- 일반주제명
- Nitrogen
- 일반주제명
- Geology
- 일반주제명
- Ecology
- 일반주제명
- Oxidation
- 일반주제명
- Carbon
- 일반주제명
- Genetic engineering
- 일반주제명
- Taxonomy
- 일반주제명
- Viruses
- 일반주제명
- Nutrients
- 일반주제명
- Viral infections
- 일반주제명
- Microorganisms
- 일반주제명
- Metabolites
- 일반주제명
- Lifestyles
- 기타저자
- California Institute of Technology Biology and Biological Engineering
- 기본자료저록
- Dissertations Abstracts International. 87-01B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
008260126s2025 us c eng d■001000017358782
■00520260202104751
■006m o d
■007cr#unu||||||||
■020 ▼a9798290657035
■035 ▼a(MiAaPQ)AAI32151341
■035 ▼a(MiAaPQ)Caltech17274
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a600
■1001 ▼aNarayanan, Aditi Kalpagam.
■24510▼aDiversity, Activity, and Adaptations of Phage Communities in Anoxic Hydrocarbon-Rich Marine Sediments
■260 ▼a[Sl]▼bCalifornia Institute of Technology▼c2025
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2025
■300 ▼a169 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 87-01, Section: B.
■500 ▼aAdvisor: Orphan, Victoria.
■5021 ▼aThesis (Ph.D.)--California Institute of Technology, 2025.
■520 ▼aThe viruses of the global ocean, especially those infecting prokaryotic taxa, are known to play an important role in maintaining the genetic and taxonomic diversity of their host communities and in the cycling of atmospheric carbon and key nutrients like nitrogen and iron. However, the vast majority of these conclusions are drawn from the surface ocean and upper water column, while the sediments, which constitute one of the largest biomes on earth, are understudied in comparison. Of special interest are areas on the ocean floor where methane and other hydrocarbons are produced and released by geological activity and oxidized by a consortium of archaea and bacteria. Using direct genomic sequencing of the viruses from a variety of simplified sediment-free enrichments of hydrocarbon oxidizers, I compare viral communities sampled from different locations and incubated under a range of temperatures to understand the role these parameters might play in shaping distribution and community structure. I then present the most comprehensive picture thus far of viral diversity and distribution from a methane cold seep and discuss whether the viral assemblages are influenced by the steep geochemical gradients that characterize seep sediments. From these datasets, I propose that viral communities in methane-oxidizing sediments are tailored specifically to the physical constraints of the sediment matrix rather than to the dominant members of the cellular community or to other physicochemical parameters such as temperature, sampling location, or depth below the seafloor. I then outline the development of two methods, stable-isotope probing coupled to nanoSIMS and biorthogonal non-canonical amino acid tagging, to work in heterogenous sediment virus samples rather than the liquid pure cultures on which they had previously relied. Implementation of these methods, which allow us to temporally constrain viral production and virus-influenced nutrient flow, resulted in the hypothesis that viral production likely responds to shifts in the major metabolic processes within an ecosystem and may influence cellular community composition.
■590 ▼aSchool code: 0037.
■650 4▼aInfections
■650 4▼aHydrocarbons
■650 4▼aChemical reactions
■650 4▼aMethane
■650 4▼aBacteria
■650 4▼aGenomes
■650 4▼aEnergy
■650 4▼aMetabolism
■650 4▼aEcosystems
■650 4▼aNitrogen
■650 4▼aGeology
■650 4▼aEcology
■650 4▼aOxidation
■650 4▼aCarbon
■650 4▼aGenetic engineering
■650 4▼aTaxonomy
■650 4▼aViruses
■650 4▼aNutrients
■650 4▼aViral infections
■650 4▼aMicroorganisms
■650 4▼aMetabolites
■650 4▼aLifestyles
■690 ▼a0372
■690 ▼a0791
■690 ▼a0329
■71020▼aCalifornia Institute of Technology▼bBiology and Biological Engineering.
■7730 ▼tDissertations Abstracts International▼g87-01B.
■790 ▼a0037
■791 ▼aPh.D.
■792 ▼a2025
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17358782▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.


