ScienceON 검색
ScienceON '특허' 검색
MORE| No | 제목 / 내용 |
|---|---|
| 1 |
METHOD FOR ENZYMATICALLY PRODUCING MAYTANSINOL / MICROBIOPHARM JAPAN CO., LTD. The present invention addresses the problem of providing a technique related to a method for enzymatically producing maytansinol from ansamitocins such as AP3. Provided is a method for producing maytansinol, the method including a step for enzymatically generating maytansinol from ansamitocins using one protein of (A)-(F) and (AA)-(CC): (A) a protein comprising the amino acid sequence represented by SEQ ID NO:9; (B) a protein comprising the amino acid sequence represented by SEQ ID NO: 9 with one or more amino acids substituted, deleted, inserted and/or added, where positions 66-68 correspond to residues DLL or AGA, positions 139-141 correspond to residues GPF, positions 146-148 correspond to residues LSS or LSP, positions 247-249 correspond to residues LHT, position 323 corresponds to residues HSX or ASX (where X is Q, K, E or M), positions 374-376 correspond to residues QSX (where X is H, R, D or Q), positions 443-445 correspond to residues GNP, and having activity for catalyzing a reaction that produces maytansinol from ansamitocins; (C) a protein having at least 50% amino acid identity with the amino acid sequence represented by SEQ ID NO: 9, where positions 66-68 correspond to residues DLL or AGA, positions 139-141 correspond to residues GPF, positions 146-148 correspond to residues LSS or LSP, positions 247-249 correspond to residues LHT, positions 323 correspond to residues HSX or ASX (where X is Q, K, E or M), positions 374-376 correspond to residues QSX (where X is H, R, D or Q), positions 443-445 correspond to residues GNP, and having activity for catalyzing a reaction that produces maytansinol from ansamitocins; (D) a protein comprising the amino acid sequence represented by SEQ ID NO: 12; (E) a protein comprising the amino acid sequence represented by SEQ ID NO: 12 with one or more amino acids substituted, deleted, inserted and/or added, where positions 66-68 correspond to residues DLL or AGA, positions 139-141 correspond to residues GPF, positions 146-148 correspond to residues LSS or LSP, positions 247-249 correspond to residues LHT, position 323 corresponds to residues HSX or ASX (where X is Q, K, E or M), positions 374-376 correspond to residues QSX (where X is H, R, D or Q), positions 443-445 correspond to residues GNP, and having activity for catalyzing a reaction that produces maytansinol from ansamitocins; (F) a protein having at least 50% amino acid identity with the amino acid sequence represented by SEQ ID NO: 12, where positions 66-68 correspond to residues DLL or AGA, positions 139-141 correspond to residues GPF, positions 146-148 correspond to residues LSS or LSP, positions 247-249 correspond to residues LHT, position 323 corresponds to residues HSX or ASX (where X is Q, K, E or M), positions 374-376 correspond to residues QSX (where X is H, R, D or Q), positions 443-445 correspond to residues GNP, and having activity for catalyzing a reaction that produces maytansinol from ansamitocins; (AA) a protein comprising the amino acid sequence represented by any one of SEQ ID NOs:18-21; (BB) a protein comprising the amino acid sequence represented by any one of SEQ ID NOs:18-21 with one or more amino acids substituted, deleted, inserted and/or added, and having activity for catalyzing a reaction that produces maytansinol from ansamitocins; and (CC) a protein having at least 80% amino acid identity with the amino acid sequence represented by any one of SEQ ID NOs:18-21, and having activity for catalyzing a reaction that produces maytansinol from ansamitocins. |
| 2 |
TRUNK LINE DISPLAY DEVICE / TOSHIBA CORP (PURPOSE) To know an extension number in specific relation with a trunk line at a glance and to carry out office work properly and also improve the service to a station calling opponent by varying and displaying the extension number at a position corresponding to a trunk line number on the display device. (CONSTITUTION) Display panels 111 , 112 ...118 on which trunk line numbers of trunk lines assigned to tenants are displayed are provided on the surface of the display device 6, and LEDs 131 , 132 ...138 as display elements capable of varying and displaying one extension number are provided at the right-hand side of the display panel. Then, a central controller CC4 is connected to an I/O port 23, latches 241 , 242 ...249 including a holding circuit for extension number data are connected to the port 23, and decoder drivers 251 , 252 ...259 are connected to those latches. Those latches 241 ...248 and drivers 251 ...258 constitute a display controller 26 which displays extension numbers on the LEDs 131 ...138 on the basis of extension number data. |
| 3 |
METHOD FOR ENZYMATICALLY PRODUCING MAYTANSINOL / MicroBiopharm Japan Co., Ltd. An object of the present invention is to provide a technique relating to a method for enzymatically producing maytansinol from an ansamitocin species such as AP3. A method for producing maytansinol, which comprises enzymatically producing maytansinol from an ansamitocin species with any one of the proteins (A) to (F) and (AA) to (CC) mentioned below: (A) a protein consisting of the amino acid sequence of SEQ ID NO: 9; (B) a protein consisting of an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 9 by substitution, deletion, insertion, and/or addition of one or more amino acids, wherein amino acids corresponding to the positions 66 to 68 are DLL or AGA, amino acids corresponding to the positions 139 to 141 are GPF, amino acids corresponding to the positions 146 to 148 are LSS or LSP, amino acids corresponding to the positions 247 to 249 are LHT, amino acids corresponding to the position 323 are HSX or ASX (where X is Q, K, E, or M), amino acids corresponding to the positions 374 to 376 are QSX (where X is H, R, D, or Q), and amino acids corresponding to the positions 443 to 445 are GNP, and having an activity for catalyzing a reaction of producing maytansinol from an ansamitocin species; (C) a protein consisting of an amino acid sequence having an identity of at least 50% to the amino acid sequence of SEQ ID NO: 9, wherein amino acids corresponding to the positions 66 to 68 are DLL or AGA, amino acids corresponding to the positions 139 to 141 are GPF, amino acids corresponding to the positions 146 to 148 are LSS or LSP, amino acids corresponding to the positions 247 to 249 are LHT, amino acids corresponding to the position 323 are HSX or ASX (where X is Q, K, E, or M), amino acids corresponding to the positions 374 to 376 are QSX (where X is H, R, D, or Q), and amino acids corresponding to the positions 443 to 445 are GNP, and having an activity for catalyzing a reaction of producing maytansinol from an ansamitocin species; (D) a protein consisting of the amino acid sequence of SEQ ID NO: 12; (E) a protein consisting of an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 12 by substitution, deletion, insertion, and/or addition of one or more amino acids, wherein amino acids corresponding to the positions 66 to 68 are DLL or AGA, amino acids corresponding to the positions 139 to 141 are GPF, amino acids corresponding to the positions 146 to 148 are LSS or LSP, amino acids corresponding to the positions 247 to 249 are LHT, amino acids corresponding to the position 323 are HSX or ASX (where X is Q, K, E, or M), amino acids corresponding to the positions 374 to 376 are QSX (where X is H, R, D, or Q), and amino acids corresponding to the positions 443 to 445 are GNP, and having an activity for catalyzing a reaction of producing maytansinol from an ansamitocin species; (F) a protein consisting of an amino acid sequence having an identity of at least 50% to the amino acid sequence of SEQ ID NO: 12, wherein amino acids corresponding to the positions 66 to 68 are DLL or AGA, amino acids corresponding to the positions 139 to 141 are GPF, amino acids corresponding to the positions 146 to 148 are LSS or LSP, amino acids corresponding to the positions 247 to 249 are LHT, amino acids corresponding to the position 323 are HSX or ASX (where X is Q, K, E, or M), amino acids corresponding to the positions 374 to 376 are QSX (where X is H, R, D, or Q), and amino acids corresponding to the positions 443 to 445 are GNP, and having an activity for catalyzing a reaction of producing maytansinol from an ansamitocin species; (AA) a protein consisting of any one of the amino acid sequences of SEQ ID NOS: 18 to 21; (BB) a protein consisting of an amino acid sequence derived from any one of the amino acid sequences of SEQ ID NOS: 18 to 21 by substitution, deletion, insertion, and/or addition of one or more amino acids, and having an activity for catalyzing a reaction of producing maytansinol from an ansamitocin species; and (CC) a protein consisting of an amino acid sequence having an identity of at least 80% to any one of the amino acid sequences of SEQ ID NOS: 18 to 21, and having an activity for catalyzing a reaction of producing maytansinol from an ansamitocin species. |
ScienceON '논문' 검색
MOREScienceON '보고서' 검색
MORE| No | 제목 / 내용 |
|---|---|
| 1 |
해수면 변동 정밀분석 및 예측 김영란 , 한국환경정책ㆍ평가연구원 , 2012 |
| 2 |
한약부작용 보고를 위한 분류코드 체계 마련 연구 윤성중 , 부산대학교 , 2018 |
| 3 |
판재 정형가공을 위한 전산원용 기술 개발 전용태 , 한국과학기술연구원 , 1994 |
| 4 |
컴퓨터를 이용한 통합생산자동화 기술 개발;사출성형금형의 생산자동화 CIM 모델플렌트 한석균 , 한국과학기술연구원 , 1991 |
| 5 |
이동 및 휴대 방송을 위한 DVB기반의 IP Datacasting(IPDC)단말 기술 개발 이재호 , (주)아이셋 , 2005 |
| 6 |
스마트카드 운영체제에 대한 EAL6 수준의 시험서 평가제출물 개발 신지훈 , 고려대학교 , 2009 |
| 7 |
비생체기반의 화재환경 유독성분 FTIR 분석법 국제표준화 여한승 , 한국화재보험협회 , 2020 |
| 8 |
대심도 연약지반의 공간적 지반특성화 및 풍화대 소켓 장대 현장타설말뚝기초의 실용화 기술개발 심성걸 , 대림산업(주) , 2007 |
| 9 |
대기오염물질 및 온실가스 통합 인벤토리 개선 연구(Ⅱ) -비도로 미산정 배출원 인벤토리 중심으로- 최성우 , 수원대학교 산학협력단 , 2015 |
| 10 |
감시정찰 센서네트워크 개발 최성우 , 한국전자통신연구원 , 2009 |